Phospho-p95/NBS1 (S343) Rabbit Recombinant mAb Datasheet

生物描述

特异性	Phospho-p95/NBS1 (S343) Rabbit Recombinant mAb detects endogenous level of total Phospho-p95/NBS1 (S343).
背景	Nbs1, a member of the Mre11-RAD50-Nbs1 complex, is phosphorylated by ATM, the product of the ataxia-telangiectasia mutated gene and a member of the phosphatidylinositol 3-kinase–related family of serine-threonine kinases, in response to DNA double-strand breaks (DSBs) to regulate DNA damage checkpoints. Nbs1 plays an important role in activation of intra-S and G2/M cell-cycle checkpoints. Nbs1 can reciprocally modulate the phosphorylation of ATM. This leads to the activation of an intra–S-phase checkpoint by phosphorylation of SMC1 and/or FANCD2 or a G2/M checkpoint via the phosphorylations of Chk2 and the phosphatase Cdc25a. In addition to the regulation of checkpoints, Nbs1 may also play a direct role in the DSB repair by HRR and NHEJ. Nbs1 is phosphorylated on S343 by ATM in the S-phase checkpoint pathway and also has been demonstrated to modulate the activity of ATM.

特异性

Phospho-p95/NBS1 (S343) Rabbit Recombinant mAb detects endogenous level of total Phospho-p95/NBS1 (S343).

背景

Nbs1, a member of the Mre11-RAD50-Nbs1 complex, is phosphorylated by ATM, the product of the ataxia-telangiectasia mutated gene and a member of the phosphatidylinositol 3-kinase–related family of serine-threonine kinases, in response to DNA double-strand breaks (DSBs) to regulate DNA damage checkpoints. Nbs1 plays an important role in activation of intra-S and G2/M cell-cycle checkpoints. Nbs1 can reciprocally modulate the phosphorylation of ATM. This leads to the activation of an intra–S-phase checkpoint by phosphorylation of SMC1 and/or FANCD2 or a G2/M checkpoint via the phosphorylations of Chk2 and the phosphatase Cdc25a. In addition to the regulation of checkpoints, Nbs1 may also play a direct role in the DSB repair by HRR and NHEJ. Nbs1 is phosphorylated on S343 by ATM in the S-phase checkpoint pathway and also has been demonstrated to modulate the activity of ATM.

使用信息

抗体应用

WB,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human

MW (kDa)

95kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Lori Rink, et al. Blood. 2007, 110(2): 651–660.

Application Data

WB

Validated by Selleck