Puromycin 2HCl

别名: CL13900 2HCl 中文名称：嘌呤霉素盐酸盐

目录号：S7417 Purity: 99.23%

Puromycin 2HCl是一种源自Streptomyces alboniger的氨基核苷类抗生素。它是蛋白质合成的抑制剂，已用于研究细胞分化过程中控制基因顺序和协调表达的转录调控机制。

Puromycin 2HCl Chemical Structure

CAS: 58-58-2

规格	价格	库存
10mM (1mL in DMSO)	RMB 573.3	现货
25mg	RMB 794.9	现货
100mg	RMB 2350.85	现货
500mg	RMB 6306.3	现货
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客户使用Selleck的Puromycin 2HCl发表文献160篇

产品质控

批次：纯度： 99.23%

99.23

常与Puromycin 2HCl一起在实验中被使用的化合物

Blasticidin S HCl

Puromycin和Blasticidin S抑制转移性去势抵抗性前列腺癌(mCRPC)细胞PC3/DU145的增殖和细胞活力。

Oseni SO, et al. bioRxiv (2021): 2021-08.

Melatonin

Melatonin通过激活HL-60细胞中的caspase-3和AMPK来增强嘌呤霉素诱导的细胞凋亡。

Koh W, et al. J Pineal Res. 2011 May;50(4):367-73.

Bortezomib

Puromycin和Bortezomib的组合显着增加了多发性骨髓瘤小鼠模型中Bortezomib的抗肿瘤活性。

Neznanov N, et al. Oncotarget. 2011 Mar; 2(3): 209–221.

Glycocholic acid

Puromycin和Glycocholate组合可增强亮氨酸脑啡肽(Leu-Enk)的吸收。

[1] Agu RU, et al. Int J Pharm. 2002 Apr 26;237(1-2):179-91.

Doxorubicin

在p53野生型HCT116细胞中，Purromycin和Doxorubicin通过抑制c-Myc和Bcl-2以及激活p53来促进细胞凋亡

Jung JH, et al. Cancers (Basel). 2019 Apr 24;11(4):582.

Puromycin 2HCl相关产品

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相关化合物库	抗感染化合物库抗生素化合物库抗病毒化合物库抗寄生虫药物库肠道微生物代谢物库	点击展开

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

细胞实验数据示例

细胞系	实验类型	孵育时间	活性描述	文献信息
HepG2	Growth inhibition assay	72 h	Growth inhibition of human HepG2 cells after 72 hrs by SRB assay, IC50=0.21 μM.	18512984
K562	Growth inhibition assay	72 h	Growth inhibition of human K562 cells after 72 hrs by SRB assay, IC50=0.22 μM.	18313307
HepG2	Growth inhibition assay	72 h	Growth inhibition of human HepG2 cells after 72 hrs by MTT assay, IC50=0.23 μM.	18313307
K562	Growth inhibition assay	72 h	Growth inhibition of doxorubicin-resistant human K562 cells after 72 hrs by SRB assay, IC50=26.83 μM.	18313307
HEK239	Cytotoxicity assay	72 h	Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay, IC50=0.42 μM.	20558060
KB3-1	Growth inhibition assay	72 hrs	Growth inhibition of human KB3-1 cells after 72 hrs by MTT assay, IC50 = 0.21 μM.	18313307
HepG2/Dox	Growth inhibition assay	72 hrs	Growth inhibition of multidrug resistant human HepG2/Dox cells after 72 hrs by SRB assay, IC50 = 20.2 μM.	18512984
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as cell viability after 72 hrs by resazurin-based plate reader analysis, IC50 = 0.361 μM.	26651537
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as growth inhibition after 72 hrs by alamar blue viability assay, IC50 = 0.3505 μM.	27060763
HEK293	Growth inhibition assay	72 hrs	Growth inhibition of HEK293 cells after 72 hrs by PrestoBlue staining based fluorescence assay, IC50 = 0.36 μM.	28001067
SU-DHL10	Cytotoxicity assay	72 hrs	Cytotoxicity against human SU-DHL10 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.11 μM.	28523103
MIAPaCa2	Cytotoxicity assay	72 hrs	Cytotoxicity against human MIAPaCa2 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.2 μM.	28523103
MDA-MB-231	Cytotoxicity assay	72 hrs	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by CellTitre-Glo luminescent assay, CC50 = 0.48 μM.	28523103
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as cell growth inhibition after 72 hrs by Alamar blue assay, IC50 = 0.46 μM.	28774427
HL60	Antiparasitic assay	72 hrs	Antiproliferative activity against human HL60 cells after 72 hrs by MTT assay, EC50 = 0.055 μM.	28803047
MOLT4	Antiparasitic assay	72 hrs	Antiproliferative activity against human MOLT4 cells after 72 hrs by MTT assay, EC50 = 0.17 μM.	28803047
Vero	Cytotoxicity assay	72 hrs	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell proliferation after 72 hrs by MTT assay, CC50 = 2.8 μM.	28803047
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells after 72 hrs by resazurin dye based assay, IC50 = 0.45 μM.	29236492
HEK293T	Cytotoxicity assay	72 hrs	Cytotoxicity against human HEK293T cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.42 μM.	29469575
HCT8	Antiparasitic assay	48 hrs	Antiparasitic activity against Cryptosporidium parvum BGF infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.55 μM.	29469575
HCT8	Antiparasitic assay	48 hrs	Antiparasitic activity against Cryptosporidium parvum SPL infected in human HCT8 cells incubated for 48 hrs by FITC/DAPI staining based fluorescence assay, EC50 = 0.6 μM.	29469575
HepG2	Cytotoxicity assay	72 hrs	Cytotoxicity against human HepG2 cells assessed as decrease in cell viability after 72 hrs by CellTiter-Glo reagent based luminescence assay, CC50 = 0.83 μM.	29469575
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against HEK293 cells assessed as inhibition of cell growth after 72 hrs by alamar blue assay, IC50 = 0.46 μM.	29533611
HEK293	Cytotoxicity assay	72 hrs	Cytotoxicity against human HEK293 cells after 72 hrs by resazurin dye based fluorescence assay, IC50 = 0.4 μM.	29969262
A549	Cytotoxicity assay	48 hrs	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM.	30418763
PC3	Cytotoxicity assay	48 hrs	Cytotoxicity against human PC3 cells assessed as decrease in cell viability after 48 hrs, IC50 = 0.3 μM.	30418763
Huh7	Cytotoxicity assay		NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7), CC50=6.63 μM.	18579783
J774A1	Cytotoxicity assay		Cytotoxicity against mouse J774A1 cells by trypan blue exclusion assay, IC50=10 μM.	22934636
A549	Cytotoxicity assay		Cytotoxicity against human A549 cells, IC50 = 0.6 μM.	27089214
PC3	Cytotoxicity assay		Cytotoxicity against human PC3 cells, IC50 = 0.7 μM.	27089214
HEK293	Cytotoxicity assay		Cytotoxicity against HEK293 cells by alamar blue assay, IC50 = 0.00035 μM.	27212070
HEK293	Growth inhibition assay		Growth inhibition of HEK293 cells, IC50 = 0.399 μM.	28400231
HCT8	Cytotoxicity assay		Cytotoxicity against human HCT8 cells, EC50 = 6.1 μM.	29469575
点击查看更多细胞系数据

生物活性

产品描述	Puromycin 2HCl是一种源自Streptomyces alboniger的氨基核苷类抗生素。它是蛋白质合成的抑制剂，已用于研究细胞分化过程中控制基因顺序和协调表达的转录调控机制。

体外研究(In Vitro)
体外研究活性	抗生素puromycin由actinoniycete和Streptornyces alboniger产生，在许多体系中被用作研究蛋白质合成的工具。^[1] Puromycin能够用于从非培养细胞中选择重组细胞。^[2]
实验图片	检测方法	检测指标	实验图片	PMID
	Western blot	Cyclin D1 / CDK4 PARP / Bcl-xL / Bcl-2 / p-Akt / Akt p53 / p21 / c-Myc		31022952
	Growth inhibition assay	Cell viability		31022952

参考文献
[1] Darken MA. Pharmacol Rev. 1964, 16, 223-243. [2] Watanabe S, et al. Biol Reprod. 2005, 72(2), 309-315. [3] Chan C, et al. Pharmacol Res Perspect. 2017 Oct;5(5):e00359. [4] Diamond JR, et al. Am J Pathol. 1986 Mar;122(3):481-7. [5] Aviner R. Comput Struct Biotechnol J. 2020 Apr 24;18:1074-1083. [6] Manabe I, et al. Circ Res. 2001;88(11):1127-1134. + 展开

参考文献

+ 展开

化学信息&溶解度

分子量	544.43	分子式	C₂₂H₂₉N₇O₅.2HCl
CAS号	58-58-2	SDF	Download Puromycin 2HCl SDF
Smiles	CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O.Cl.Cl
储存条件(自收到货起)	3年-20°C粉状	储备液保存和期限建议分装储备液，避免反复冻融！	1年-80°C溶于溶剂
储存条件(自收到货起)	3年-20°C粉状	储备液保存和期限建议分装储备液，避免反复冻融！	1个月-20°C溶于溶剂

体外溶解度
批次：

DMSO : 100 mg/mL ( (183.67 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Water : 100 mg/mL (183.67 mM)

Ethanol : Insoluble

摩尔浓度计算器

体内溶解度
批次：

现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂

动物体内配方计算器

实验计算

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×

稀释计算器分子量计算器

动物体内配方计算器（澄清溶液）

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 μL 动物数量只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

计算结果：

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH₂O，混匀澄清。

体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。

注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

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