FCCP
别名: Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone 中文名称：碳酰氰-4-三氟甲氧基苯腙
FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone)在线粒体中是一种有效的氧化磷酸化的解偶联剂，通过转运质子破坏ATP的合成。
FCCP Chemical Structure
CAS: 370-86-5
产品质控
批次：
纯度：
99.99%
99.99
FCCP相关产品
| 相关产品 | IACS-010759 (IACS-10759) Gboxin VLX600 S-Gboxin | 点击展开 |
|---|---|---|
| 相关化合物库 | 自噬化合物库 凋亡分子化合物库 铁死亡化合物库 细胞焦亡化合物库 线粒体靶向化合物库 | 点击展开 |
细胞实验数据示例
| 细胞系 | 实验类型 | 给药浓度 | 孵育时间 | 活性描述 | 文献信息 |
|---|---|---|---|---|---|
| T47D | Function assay | 0.3 uM | 15 mins | Decrease in mitochondrial membrane potential in human T47D cells 0.3 uM after 15 mins by TMRM assay | 20929261 |
| T47D | Function assay | 3 uM | 15 to 20 mins | Decrease in mitochondrial membrane potential in human T47D cells at 3 uM after 15 to 20 mins by TMRM assay | 22938093 |
| SH-SY5Y | Function assay | 10 uM | 5 mins | Inhibition of SOC in human SH-SY5Y cells assessed as reduction in thapsigargin-induced Ca2+ influx at 10 uM pre-incubated for 5 mins with 0.2 uM CsA followed by compound addition by FURA-2AM dye based fluorescence assay | 25265024 |
| SH-SY5Y | Function assay | 10 uM | 10 mins | Induction of mitochondrial membrane potential loss in human SH-SY5Y cells at 10 uM incubated for 10 mins in presence of 0.2 uM CsA by TMRE dye based assay | 25265024 |
| T47D | Function assay | 0.3 uM | 3 to 12 mins | Increase in oxygen consumption rate of mitochondrial state 4 respiration in human T47D cells assessed as reinitiation of oligomycin-stalled cellular respiration at 0.3 uM incubated for 3 to 12 mins by Clark-type oxygen electrode assay | 26637046 |
| T47D | Function assay | 0.3 uM | 30 mins | Effect on mitochondrial membrane potential in human T47D cells at 0.3 uM after 30 mins by TMRM dye based fluorescence microscopy | 26637046 |
| HCT116 | Function assay | 2 uM | 30 mins | Induction of AMPK phosphorylation at Thr-172 residue in human HCT116 cells at 2 uM after 30 mins in glucose supplemented media by immunoblot method | 28233680 |
| HCT116 | Function assay | 2 uM | 30 mins | Induction of AMPK phosphorylation at Thr-172 residue in human HCT116 cells at 2 uM after 30 mins in absence of glucose by immunoblot method | 28233680 |
| KOPN8 | Function assay | 10 uM | 0.3 hrs | Induction of mitochondrial membrane potential loss in human KOPN8 cells at 10 uM after 0.3 hrs by TMRM staining based flow cytometric analysis | 31084028 |
| T47D | Function assay | 1 to 10 uM | Inhibition of HIF1-mediated induction of secreted VEGF level in 1, 10-phenanthroline-stimulated human T47D cells at 1 to 10 uM by ELISA | 20929261 | |
| MDA-MB-231 | Cytotoxicity assay | 1 uM | Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation/viability at 1 uM | 23245650 | |
| MDA-MB-231 | Cytotoxicity assay | 0.1 to 3 uM | Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation/viability at 0.1 to 3 uM in presence of 0.1 uM rotenone mitochondrial electron transport inhibitor | 23245650 | |
| MDA-MB-231 | Function assay | 0.3 uM | Stimulation of oligomycin-induced state 4 respiration in human MDA-MB-231 cells at 0.3 uM | 23245650 | |
| T47D | Function assay | 0.1 to 3 uM | Effect on cellular respiration in human T47D cells assessed as increase in oxygen consumption at 0.1 to 3 uM | 22938093 | |
| T47D | Function assay | 10 to 30 uM | Stimulation of state 4 cellular respiration in human T47D cells at 10 to 30 uM in presence of oligomycin | 22938093 | |
| T47D | Function assay | 0.3 to 1 uM | Stimulation of state 4 cellular respiration in human T47D cells at 0.3 to 1 uM in presence of oligomycin | 22938093 | |
| Hep3B | Function assay | 10 uM | Stimulation of state 4 cellular respiration in human Hep3B cells at 10 uM in presence of oligomycin | 22938093 | |
| Hep3B | Function assay | 1 uM | Stimulation of state 4 cellular respiration in human Hep3B cells at 1 uM in presence of oligomycin | 22938093 | |
| TA3/Ha | Function assay | 6 uM | Induction of NAD(P)H oxidation in mouse TA3/Ha cells assessed as reduction of NAD(P)H/NAD(P)+ ratio at 6 uM by spectrofluorometer analysis | 24568614 | |
| T47D | Function assay | 0.3 uM | Increase in oxygen consumption rate in digitonin permeabilized human T47D cells assessed as reinitiation of sodium azide-stalled cellular respiration at 0.3 uM by oxytherm Clark-type electrode assay in presence of ascorbate | 26637046 | |
| DLD1 | Function assay | 1 uM | Induction of mitochondrial dysfunction in human DLD1 cells assessed as reduction in mitochondrial ATP production at 1 uM by Seahorse XF real-time assay | 31774672 | |
| DLD1 | Function assay | 1 uM | Induction of mitochondrial dysfunction in human DLD1 cells assessed as increase in glycolytic ATP production at 1 uM by Seahorse XF real-time assay | 31774672 | |
| LS174T | Function assay | 1 uM | Induction of mitochondrial dysfunction in human LS174T cells assessed as reduction in mitochondrial ATP production at 1 uM by Seahorse XF real-time assay | 31774672 | |
| LS174T | Function assay | 1 uM | Induction of mitochondrial dysfunction in human LS174T cells assessed as increase in glycolytic ATP production at 1 uM by Seahorse XF real-time assay | 31774672 | |
| DLD1 | Function assay | 1 uM | Uncoupling of mitochondrial oxidative phosphorylation in human DLD1 cells at 1 uM in presence of oligomycin A by seahorse XFe96 analyser based assay | 31774672 | |
| DLD1 | Function assay | 1 uM | Uncoupling of mitochondrial oxidative phosphorylation in human DLD1 cells assessed as increase in oxygen consumption rate at 1 uM in presence of oligomycin A by seahorse XFe96 analyser based assay | 31774672 | |
| HEK293 | Function assay | 1 to 3 uM | Inhibition of LiCl-activated Wnt signaling in HEK293 cells at 1 to 3 uM by TOPFlash reporter gene assay | 31774672 | |
| T47D | Function assay | Inhibition of hypoxia-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay, IC50=0.51μM | 20929261 | ||
| T47D | Function assay | Inhibition of 1, 10-phenanthroline-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay, IC50=0.31μM | 20929261 | ||
| HepG2 | Function assay | Luciferase/luciferin-expressing antifolate-resistant parasites were used to infect a culture of HepG2 cells that were pre-incubated with compounds. Infected hepatocytes emit light due to the luciferase reaction. Assay results are presented as the percent , IC50=0.245μM | ChEMBL | ||
| 点击查看更多细胞系数据 | |||||
生物活性
| 产品描述 | FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone)在线粒体中是一种有效的氧化磷酸化的解偶联剂，通过转运质子破坏ATP的合成。 | ||
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| 靶点 |
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| 体外研究(In Vitro) | ||||
| 体外研究活性 | 在体外实验中，FCCP的处理能诱导细胞内Ca2+迅速升至2倍，同时抑制蛋白合成速率。对蛋白翻译速率的抑制作用与eIF2α的磷酸化增加以及依赖双链RNA的蛋白激酶活性增加有关[1]。 FCCP还可轻微地减少ATP以及活性氧水平，提高线粒体基因如Tfam和COXIV的表达，诱导老鼠造血干细胞的静态形态特征以及抑制TGF-β的信号转导[2]。 |
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|---|---|---|---|---|
| 细胞实验 | 细胞系 | PC12细胞 | ||
| 浓度 | 30 μM | |||
| 孵育时间 | 30 min, 1h, 2h | |||
| 方法 | 用直径为24-mm的多孔板，加入含0.175 Ci/mmol的[3H]methionine的新鲜培养液，37℃孵育30分钟。在不同的时间段对PC12细胞处理以FCCP，测定蛋白质合成速率。 |
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| 体内研究(In Vivo) | ||
| 体内研究活性 | FCCP的处理在8细胞期的小鼠胚胎中可显著降低线粒体膜电位、ATP的生成以及囊胚中细胞内细胞团的数量，对胚泡发育没有影响。在胚胎线粒体功能受到干扰的同时，在胚胎植入后，雌性后代的出生体重下降，抑制持续到断奶阶段。尽管FCCP处理过的雄性小鼠也和雌性小鼠一样，葡萄糖耐受量降低。但是它们的胰岛素敏感度和肥胖度在4-14周内无变化。在预压胚中，线粒体功能降低，然后减少ATP的输出，将影响其后代表型[3]。 |
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|---|---|---|
化学信息&溶解度
| 分子量 | 254.17 | 分子式 | C10H5F3N4O |
| CAS号 | 370-86-5 | SDF | Download FCCP SDF |
| Smiles | C1=CC(=CC=C1NN=C(C#N)C#N)OC(F)(F)F | ||
| 储存条件(自收到货起) | |||
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体外溶解度 |
DMSO : 6 mg/mL ( (23.6 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO) Water : Insoluble Ethanol : Insoluble |
摩尔浓度计算器 |
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体内溶解度 现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂 |
动物体内配方计算器 | ||||
实验计算
动物体内配方计算器（澄清溶液）
第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）
mg/kg
g
μL
只
第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）
% DMSO
%
% Tween 80
% ddH2O
%DMSO
%
计算结果：
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；
体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH2O，混匀澄清。
体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。
注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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