14-3-3 epsilon Rabbit Recombinant mAb Datasheet

生物描述

特异性	14-3-3 epsilon Rabbit Recombinant mAb detects endogenous level of total 14-3-3 epsilon.
背景	The 14-3-3 proteins are a family of conserved regulatory molecules expressed in all eukaryotic cells. They could bind a multitude of functionally diverse signaling proteins, including kinases, phosphatases, and transmembrane receptors and plays important roles in a wide range of vital regulatory processes, such as mitogenic signal transduction, apoptotic cell death, and cell cycle control. 14-3-3 is present in almost all tissues, including testes, liver, and heart. Within a eukaryotic cell, 14-3-3 is largely found in the cytoplasmic compartment. However, 14-3-3 proteins can also be detected at the plasma membrane and in intracellular organelles such as the nucleus and the Golgi apparatus. As a member of the 14-3-3 protein family, 14-3-3ε may integrate a number of signaling pathways by interacting with distinct proteins; therefore, 14-3-3ε serves functions in the regulation of the cell cycle and apoptosis. It binds with heat shock factor 1 (HSF1) through the extracellular signal regulated kinase/glycogen synthase kinase 3/14-3-3ε pathway, thus suppressing HSF1 activity during cellular proliferation. Inhibiting the interaction of 14-3-3ε with chloride intracellular channel protein 4 enhanced autophagy and contributed to mitochondrial and ER stress-induced apoptosis under starvation conditions. In addition, 14-3-3ε has an association with the occurrence and development of a number of types of tumor; for example, the expression of 14-3-3ε has been identified to be downregulated in gastric cancer In addition, 14-3-3ε has an association with the occurrence and development of a number of types of tumor; for example, the expression of 14-3-3ε has been identified to be downregulated in gastric cancer and pediatric astrocytoma, whereas it is upregulated in hepatocellular carcinoma, renal cancer and breast cancer. 14-3-3ε is essential for normal brain development and neuronal migration in the mouse. This function of 14-3-3ε seems to be accomplished at least in part by binding to CDK5/p35 phosphorylated NUDEL to maintain NUDEL phosphorylation.

特异性

14-3-3 epsilon Rabbit Recombinant mAb detects endogenous level of total 14-3-3 epsilon.

背景

The 14-3-3 proteins are a family of conserved regulatory molecules expressed in all eukaryotic cells. They could bind a multitude of functionally diverse signaling proteins, including kinases, phosphatases, and transmembrane receptors and plays important roles in a wide range of vital regulatory processes, such as mitogenic signal transduction, apoptotic cell death, and cell cycle control. 14-3-3 is present in almost all tissues, including testes, liver, and heart. Within a eukaryotic cell, 14-3-3 is largely found in the cytoplasmic compartment. However, 14-3-3 proteins can also be detected at the plasma membrane and in intracellular organelles such as the nucleus and the Golgi apparatus. As a member of the 14-3-3 protein family, 14-3-3ε may integrate a number of signaling pathways by interacting with distinct proteins; therefore, 14-3-3ε serves functions in the regulation of the cell cycle and apoptosis. It binds with heat shock factor 1 (HSF1) through the extracellular signal regulated kinase/glycogen synthase kinase 3/14-3-3ε pathway, thus suppressing HSF1 activity during cellular proliferation. Inhibiting the interaction of 14-3-3ε with chloride intracellular channel protein 4 enhanced autophagy and contributed to mitochondrial and ER stress-induced apoptosis under starvation conditions. In addition, 14-3-3ε has an association with the occurrence and development of a number of types of tumor; for example, the expression of 14-3-3ε has been identified to be downregulated in gastric cancer In addition, 14-3-3ε has an association with the occurrence and development of a number of types of tumor; for example, the expression of 14-3-3ε has been identified to be downregulated in gastric cancer and pediatric astrocytoma, whereas it is upregulated in hepatocellular carcinoma, renal cancer and breast cancer. 14-3-3ε is essential for normal brain development and neuronal migration in the mouse. This function of 14-3-3ε seems to be accomplished at least in part by binding to CDK5/p35 phosphorylated NUDEL to maintain NUDEL phosphorylation.

使用信息

抗体应用

WB ,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

29kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck