14-3-3 sigma Rabbit Recombinant mAb Datasheet

生物描述

特异性	14-3-3 sigma Rabbit Recombinant mAb detects endogenous level of total 14-3-3 sigma.
背景	The 14-3-3σ (sigma) protein, a negative regulator of the cell cycle, is a human mammary epithelium-specific marker. 14-3-3σ is a p53-inducible gene product involved in cell cycle checkpoint control after DNA damage. 14-3-3σ gene is not expressed in most breast cancers (9), gastric cancer (37), or hepatocellular carcinoma. Since 14-3-3σ is regulated by p53 to guard genomic stability and is required to prevent mitotic catastrophe in response to DNA damage, dysregulation of 14-3-3σ may account for some of the cancers that do not have p53 mutations. 14-3-3σ also interacts with CDKs and can inhibit CDK activities to block cell cycle progression. Overexpression of 14-3-3σ also inhibits cell proliferation and prevents anchorage-independent growth of breast cancer cell lines.

特异性

14-3-3 sigma Rabbit Recombinant mAb detects endogenous level of total 14-3-3 sigma.

背景

The 14-3-3σ (sigma) protein, a negative regulator of the cell cycle, is a human mammary epithelium-specific marker. 14-3-3σ is a p53-inducible gene product involved in cell cycle checkpoint control after DNA damage. 14-3-3σ gene is not expressed in most breast cancers (9), gastric cancer (37), or hepatocellular carcinoma. Since 14-3-3σ is regulated by p53 to guard genomic stability and is required to prevent mitotic catastrophe in response to DNA damage, dysregulation of 14-3-3σ may account for some of the cancers that do not have p53 mutations. 14-3-3σ also interacts with CDKs and can inhibit CDK activities to block cell cycle progression. Overexpression of 14-3-3σ also inhibits cell proliferation and prevents anchorage-independent growth of breast cancer cell lines.

使用信息

抗体应用

WB ,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Rat

MW (kDa)

28kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck