AF488 mouse/human/rat Histone H3 Antibody [D1H2]

目录号：G5049

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生物描述

特异性
背景 组蛋白H3 是核小体的核心成分，核小体是染色质的基本单位，DNA包裹在组蛋白八聚体周围。组蛋白H3的氨基末端尾部经历多种翻译后修饰，包括乙酰化、磷酸化、甲基化和泛素化，这些修饰影响染色质的可及性和基因表达。在大多数物种中，H3在Lys9、Lys14、Lys18、Lys23、Lys27和Lys56处被乙酰化。组蛋白H3在Ser10、Ser28和Thr11处的磷酸化与有丝分裂和减数分裂期间的染色体凝集密切相关。

使用信息

抗体应用 FCM, IHC, IF 推荐用量 0.2 μg ( 0.4 μL) / million cells in 100 µL volume
反应性 Human, Mouse, Rat, Monkey, Hamster, Chicken, D. melanogaster, Xenopus, Zebrafish, Bovine, Feline
抗体类型 Rabbit MW 17 kDa
储存液配方 Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
储存条件
(自收到货起)		 Store at 4°C in the dark to avoid freeze-thaw cycles

Application Data

FCM

Validated by Selleck

  • Fixation of Hela cells with 4% formaldehyde, after washing, cells were permeabilized by adding 1000 μL of pre-cooled 90% methanol, followed by washing, and then staining of cells using AF488 mouse/human/rat Histone H3 Antibody [D1H2] (Selleck Cat.: G5049) (green, with red blank control). Analyzed data are derived from all cells with intact scatter.