AF488 mouse/human/rat p44/42 MAPK Antibody [B5N16]

目录号：G4974

打印

生物描述

特异性
背景 p44/42 MAPK（Erk1/2）通路，属于高度保守的丝裂原活化蛋白激酶家族，该家族是调节细胞增殖、分化、运动和死亡等关键细胞过程的丝氨酸/苏氨酸蛋白激酶。该通路响应多种胞外信号（如丝裂原、生长因子和细胞因子）而被激活，是癌症诊断和治疗的关键靶点。其激活通过三级激酶级联反应发生：MAP激酶激酶激酶（MAP3K）、MAP激酶激酶（MAP2K）和MAPK。

使用信息

抗体应用 FCM, IHC, IF 推荐用量 0.2 μg ( 0.4 μL) / million cells in 100 µL volume
反应性 Human, Mouse, Rat, Hamster, Monkey, Mink, D. melanogaster, Zebrafish, Bovine, Dog, Pig, C. elegans, Chicken
抗体类型 Rabbit MW 42 kDa, 44 kDa
储存液配方 Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
储存条件
(自收到货起)		 Store at 4°C in the dark to avoid freeze-thaw cycles

Application Data

FCM

Validated by Selleck

  • Fixation of Jurkat cells with 4% formaldehyde, after washing, cells were permeabilized by adding 1000 μL of pre-cooled 90% methanol, followed by washing, and then staining of cells using AF488 mouse/human/rat p44/42 MAPK Antibody [B5N16] (Selleck Cat.: G4974) (green, with red blank control). Analyzed data are derived from all cells with intact scatter.