AF488 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody [D13L13]

目录号：G3729

打印

生物描述

特异性
背景 丝裂原活化蛋白激酶（MAPK）是一个广泛保守的丝氨酸/苏氨酸蛋白激酶家族，参与许多细胞程序，如细胞增殖、分化、运动和死亡。p44/42 MAPK（Erk1/2）信号通路可响应多种胞外刺激（包括丝裂原、生长因子和细胞因子）而被激活，研究人员认为它是癌症诊断和治疗的重要靶点。刺激后，启动一个顺序的三部分蛋白激酶级联反应，由MAP激酶激酶激酶（MAPKKK或MAP3K）、MAP激酶激酶（MAPKK或MAP2K）和MAP激酶（MAPK）组成。

使用信息

抗体应用 FCM, IHC, IF 推荐用量 0.2 μg (0.4 μL) / million cells in 100 µL volume
反应性 Human, Mouse, Rat, Hamster, Monkey, Mink, D. melanogaster, Zebrafish, Bovine, Dog, Pig, S. cerevisiae, Chicken, C. elegans
抗体类型 Rabbit MW 44 kDa, 42 kDa
储存液配方 Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
储存条件
(自收到货起)		 Store at 4°C in the dark to avoid freeze-thaw cycles

Application Data

FCM

Validated by Selleck

  • Jurkat cells were treated with TPA (200 nM, treated for 30 min). Then cells were stained using AF488 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody [D13L13] (Selleck Cat.: G3729) (green, with red blank control). Analyzed data are derived from live total cells.