AF647 Human CD152/CTLA-4 Antibody [BNI3]

目录号：H1362

打印

生物描述

特异性
背景 CTLA-4，也称为CD152，在活化的CD28+ T细胞上瞬时表达，并以高亲和力结合抗原呈递细胞（APC）上存在的CD80和CD86。这种相互作用似乎向T细胞传递负调节信号。CTLA-4也在与活化T细胞共培养的B细胞上表达，表明CTLA-4在B细胞应答的调节中发挥作用。抗CTLA-4的抗体增强由CD3和CD28的抗体介导的交联诱导的T细胞增殖。CD152可由调节性T（Treg）细胞表达。细胞固定和破膜后，抗CTLA-4的抗体可以染色T细胞（包括Treg细胞）中表达的细胞内CD152。

使用信息

抗体应用 FCM, IHC 推荐用量 5 uL/million cells in 100 µL volume
反应性 Human , Rhesus, Cynomolgus, Baboon
抗体类型 Mouse MW
储存液配方 Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
储存条件
(自收到货起)		 Store at 4°C in the dark to avoid freeze-thaw cycles

Application Data

FCM

Validated by Selleck

  • Staining HEK-293F cells transfected with pEnCMV-CTLA4(human)-1-3×FLAG-SV40-Neo plasmid and fixed/permeabilized with fixation/permeabilization buffer with AF647 Human CD152/CTLA-4 Antibody (Clone: BNI3) (Product Number: H1362) (green, red is unstained control). Analysis was performed on total cells.