APC Mouse/Human/Rat FOXP3 Antibody [FJK-16s]

目录号：G0033

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生物描述

特异性
背景 FOXP3（叉头盒蛋白3）是叉头/翼状螺旋转录调节因子家族的成员，在哺乳动物中高度保守，对于正常的免疫稳态至关重要。FOXP3长381个氨基酸，在CD25+ CD4阳性调节性T细胞中高水平稳定且持续表达，在CD4阳性/CD25阴性细胞中低水平表达，在CD4阴性/CD8阳性T细胞中缺失。FOXP3可能是一个主调控基因，也是调节性T细胞更特异的标记物。编码FOXP3蛋白的基因缺陷会导致小鼠出现scurfy表型。

使用信息

抗体应用 FCM, IHC 推荐用量 0.2 μg (1 μL) / million cells in 100 µL volume
反应性 Bovine, Dog, Cat, Mouse, Pig, Rat, Cynomolgus monkey, Ferret, Horse, Human, Sheep
抗体类型 Rat MW 49-55 kDa
储存液配方 Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
储存条件
(自收到货起)		 Store at 4°C in the dark to avoid freeze-thaw cycles

Application Data

FCM

Validated by Selleck

  • Staining of C57BL/6 mouse splenocytes with FITC CD4 Antibody for surface staining, after washing, cells were permeabilized using Foxp3/Transcription Factor Staining Buffer Set (Permeabilization Reagent), and then stained for intranuclear staining with APC Mouse/Human/Rat FOXP3 Antibody [FJK-16s] (Selleck Cat.: G0033). Analyzed data are derived from cells within the lymphocyte gate.