Apolipoprotein E Rabbit Recombinant mAb Datasheet

生物描述

特异性	Apolipoprotein E Rabbit Recombinant mAb detects endogenous level of total Apolipoprotein E.
背景	Apolipoprotein (apo) E is a multifunctional protein with central roles in lipid metabolism, neurobiology, and neurodegenerative diseases. It has three major isoforms (apoE2, apoE3, and apoE4) with different effects on lipid and neuronal homeostasis. ApoE participates in the distribution/redistribution of lipids among various tissues and cells of the body via mediating the binding of lipoproteins or lipid complexes in the plasma or interstitial fluids to specific cell-surface receptors. The low-density lipoprotein receptor (LDLR) family, including LDLR, LDLR-related protein 1 (LRP1), VLDL receptor (VLDLR), and apoE receptor 2 (apoER2), are the major apoE receptors. ApoE is synthesized and secreted from a variety of tissues and several types of cells and is abundant in the interstitial fluid and lymph, as well as in the plasma. It is mainly produced by hepatocytes, macrophages, and adipocytes in the peripheral tissues. ApoE in the plasma is preferentially associated with very low density lipoprotein (VLDL) particles, whereas it is found in high-density lipoprotein–like particles in the CNS.

特异性

Apolipoprotein E Rabbit Recombinant mAb detects endogenous level of total Apolipoprotein E.

背景

Apolipoprotein (apo) E is a multifunctional protein with central roles in lipid metabolism, neurobiology, and neurodegenerative diseases. It has three major isoforms (apoE2, apoE3, and apoE4) with different effects on lipid and neuronal homeostasis. ApoE participates in the distribution/redistribution of lipids among various tissues and cells of the body via mediating the binding of lipoproteins or lipid complexes in the plasma or interstitial fluids to specific cell-surface receptors. The low-density lipoprotein receptor (LDLR) family, including LDLR, LDLR-related protein 1 (LRP1), VLDL receptor (VLDLR), and apoE receptor 2 (apoER2), are the major apoE receptors. ApoE is synthesized and secreted from a variety of tissues and several types of cells and is abundant in the interstitial fluid and lymph, as well as in the plasma. It is mainly produced by hepatocytes, macrophages, and adipocytes in the peripheral tissues. ApoE in the plasma is preferentially associated with very low density lipoprotein (VLDL) particles, whereas it is found in high-density lipoprotein–like particles in the CNS.

使用信息

抗体应用

WB ,ELISA,ELISA

稀释比例

WB

1:500

反应性

Human Mouse

MW (kDa)

36kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck