Cdc23/APC8 Rabbit Recombinant mAb Datasheet

生物描述

特异性	Cdc23/APC8 Rabbit Recombinant mAb detects endogenous level of total Cdc23/APC8.
背景	The anaphase-promoting complex or cyclosome (APC/C) is a multi-subunit E3 ubiquitin ligase whose primary function is to control cell cycle progression by mediating the destruction of proteins whose activities inhibit specific cell cycle transitions. APC/C catalytic activity is conferred by the RING domain subunit Apc11 that is tightly associated with the cullin subunit Apc2. Together with the coactivator subunit and Apc10, these four subunits constitute the catalytic and substrate recognition module of the complex. All other subunits provide scaffolding functions to coordinate the organization of the catalytic and substrate recognition subunits and also act to mediate interactions with the mitotic checkpoint complex. The three canonical tetratricopeptide repeat (TPR) subunits (Cdc16/Apc6, Cdc23/Apc8 and Cdc27/Apc3) are homo-dimers. Cdc23 forms a homo-dimer structurally related to Cdc16 and Cdc27. It engages the coactivator subunits responsible for substrate recognition. CDC23 may be involved in thyroid cancer initiation and progression. It is differentially expressed by gender and is overexpressed in papillary thyroid cancer.

特异性

Cdc23/APC8 Rabbit Recombinant mAb detects endogenous level of total Cdc23/APC8.

背景

The anaphase-promoting complex or cyclosome (APC/C) is a multi-subunit E3 ubiquitin ligase whose primary function is to control cell cycle progression by mediating the destruction of proteins whose activities inhibit specific cell cycle transitions. APC/C catalytic activity is conferred by the RING domain subunit Apc11 that is tightly associated with the cullin subunit Apc2. Together with the coactivator subunit and Apc10, these four subunits constitute the catalytic and substrate recognition module of the complex. All other subunits provide scaffolding functions to coordinate the organization of the catalytic and substrate recognition subunits and also act to mediate interactions with the mitotic checkpoint complex. The three canonical tetratricopeptide repeat (TPR) subunits (Cdc16/Apc6, Cdc23/Apc8 and Cdc27/Apc3) are homo-dimers. Cdc23 forms a homo-dimer structurally related to Cdc16 and Cdc27. It engages the coactivator subunits responsible for substrate recognition. CDC23 may be involved in thyroid cancer initiation and progression. It is differentially expressed by gender and is overexpressed in papillary thyroid cancer.

使用信息

抗体应用

WB ,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

64kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck