Cyclin H Rabbit Recombinant mAb

生物描述

特异性

Cyclin H Rabbit Recombinant mAb detects endogenous level of total Cyclin H.

背景

Cell cycle progression is tightly regulated by cyclin dependent kinases (cdks). The activity of these kinases is controlled by phosphorylation and dephosphorylation events, by binding of the regulatory subunits, namely the cyclins to the catalytic cdk-subunits, as well as by the interaction of inhibitor molecules with the cyclin/cdk-complexes. Cyclin H is a phosphoprotein and one of the kinases that phosphorylate cyclin H was identified as cyclin C/cdk8. Cyclin H is a substrate for another protein kinase namely protein kinase CK2 and behaves very similar to the majority of other CK2 substrates. cyclin H forms a complex with CDK7 kinase and ring finger protein MAT1. The complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). Cyclin H and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes.

使用信息

抗体应用	WB, IF,ELISA,ELISA
稀释比例	WB IF 1:1000 1:50
反应性	Human Mouse
MW (kDa)	38kDa
抗体类型	Rabbit
储存液配方	10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
储存条件 (自收到货起)	Store at –20°C.

文献参考

[1] Schneider E, et al. Oncogene. 2002, 21(33):5031-7.

Application Data

WB

IF