Fibronectin Rabbit Recombinant mAb Datasheet

生物描述

特异性	Fibronectin Rabbit Recombinant mAb detects endogenous level of total Fibronectin.
背景	Fibronectin (FN) mediates a wide variety of cellular interactions with the extracellular matrix (ECM) and plays important roles in cell adhesion, migration, growth and differentiation. FN is widely expressed by multiple cell types and is critically important in vertebrate development. FN usually exists as a dimer composed of two nearly identical ∼250 kDa subunits linked covalently near their C-termini by a pair of disulfide bonds. It can be a ligand for a dozen members of the integrin receptor family. FN has a remarkably wide variety of functional activities besides binding to cell surfaces through integrins. It binds to a number of biologically important molecules that include heparin, collagen/gelatin, and fibrin. Although the plasma FN that circulates in blood is in a closed, reportedly non-active form, most of the FN activities in the body have been ascribed to the insoluble form of FN that exists as part of the extracellular matrix.

特异性

Fibronectin Rabbit Recombinant mAb detects endogenous level of total Fibronectin.

背景

Fibronectin (FN) mediates a wide variety of cellular interactions with the extracellular matrix (ECM) and plays important roles in cell adhesion, migration, growth and differentiation. FN is widely expressed by multiple cell types and is critically important in vertebrate development. FN usually exists as a dimer composed of two nearly identical ∼250 kDa subunits linked covalently near their C-termini by a pair of disulfide bonds. It can be a ligand for a dozen members of the integrin receptor family. FN has a remarkably wide variety of functional activities besides binding to cell surfaces through integrins. It binds to a number of biologically important molecules that include heparin, collagen/gelatin, and fibrin. Although the plasma FN that circulates in blood is in a closed, reportedly non-active form, most of the FN activities in the body have been ascribed to the insoluble form of FN that exists as part of the extracellular matrix.

使用信息

抗体应用

WB, IHC,ELISA,ELISA

稀释比例

WB	IHC
1:1000	1:50

反应性

Human

MW (kDa)

263kDa

抗体类型

Rabbit

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

[1] Roumen Pankov, et al. Journal of Cell Science. 2002, 115: 3861-3863.

Application Data

WB

Validated by Selleck

IHC

Validated by Selleck