Datasheet

生物描述

特异性	MBD2 Rabbit Recombinant mAb detects endogenous levels of total MBD2.
背景	DNA methylation is an epigenetic mark that is essential for many biological processes and is linked to diseases such as cancer. The methyl-CpG-binding domain (MBD) family of proteins bind to methylated DNA and can aid in the mediation of gene silencing through interaction with histone deacetylases and histone methyltransferases. They are a key component of epigenetic regulation and dynamic readers of methylation beyond their originally proposed roles as transcriptional repressors. The MBD protein family consists of MeCP2 and MBD1-6. MBD2 is highly conserved, ubiquitously expressed, and interacts with the nucleosome remodeling and histone deacetylation (NuRD) complex. NuRD consists of the ATP-dependent remodeling enzymes CHD3/4, histone deacetylases HDAC1/2, histone chaperones RBBP4/7, and DNA binding proteins GATAD2A/B and MTA1/2/3 in addition to MBD2 and MBD3, and has both ATP-dependent nucleosome remodeling and histone deacetylase activity. MBD2 recruits NuRD to methylated regions of the genome to induce histone deacetylase-dependent transcriptional silencing and chromatin compaction. MBD2 is also found at unmethylated, actively transcribed genes and requires interaction with the NuRD complex for this localization. Thus, MBD2, through its interactions with NuRD, may be involved in transcriptional activation as well as repression, and may be localized at unmethylated sites. In addition to NuRD co-repressor complex, MDB2 can also interact with other diverse protein complexes, such as PRMT1, PRMT5, TACC3 and pCAF. It may have neuronal functions, play roles in pluripotent cells, and immunity, also in cancer.

特异性

MBD2 Rabbit Recombinant mAb detects endogenous levels of total MBD2.

背景

DNA methylation is an epigenetic mark that is essential for many biological processes and is linked to diseases such as cancer. The methyl-CpG-binding domain (MBD) family of proteins bind to methylated DNA and can aid in the mediation of gene silencing through interaction with histone deacetylases and histone methyltransferases. They are a key component of epigenetic regulation and dynamic readers of methylation beyond their originally proposed roles as transcriptional repressors. The MBD protein family consists of MeCP2 and MBD1-6. MBD2 is highly conserved, ubiquitously expressed, and interacts with the nucleosome remodeling and histone deacetylation (NuRD) complex. NuRD consists of the ATP-dependent remodeling enzymes CHD3/4, histone deacetylases HDAC1/2, histone chaperones RBBP4/7, and DNA binding proteins GATAD2A/B and MTA1/2/3 in addition to MBD2 and MBD3, and has both ATP-dependent nucleosome remodeling and histone deacetylase activity. MBD2 recruits NuRD to methylated regions of the genome to induce histone deacetylase-dependent transcriptional silencing and chromatin compaction. MBD2 is also found at unmethylated, actively transcribed genes and requires interaction with the NuRD complex for this localization. Thus, MBD2, through its interactions with NuRD, may be involved in transcriptional activation as well as repression, and may be localized at unmethylated sites. In addition to NuRD co-repressor complex, MDB2 can also interact with other diverse protein complexes, such as PRMT1, PRMT5, TACC3 and pCAF. It may have neuronal functions, play roles in pluripotent cells, and immunity, also in cancer.

使用信息

抗体应用

WB, IF,ELISA,ELISA

稀释比例

WB	IF
1:1000	1:50

反应性

Human Mouse Rat

MW (kDa)

29,43kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck

IF

Validated by Selleck