Datasheet

生物描述

特异性	NEDD4-2 Rabbit Recombinant mAb detects endogenous levels of total NEDD4-2.
背景	Nedd4-2 is a ubiquitin protein ligase of the Nedd4 family which is known to bind and regulate a number of membrane proteins to aid in their internalization and turnover. Several of the NEDD4-2 substrates include ion channels, such as the epithelial and voltage-gated sodium channels. NEDD4-2 plays critial roles in regulating membrane proteins and is essential for the maintenance of cellular homeostasis. Nedd4-2 is an E3 ubiquitin-protein ligase that reduces expression of the epithelial Na+ channel ENaC at the cell surface. Nedd4-2 catalyzes ubiquitination of α-, β-, and γENaC. Its overexpression increases ubiquitination, whereas Nedd4-2 silencing decreases ubiquitination.

特异性

NEDD4-2 Rabbit Recombinant mAb detects endogenous levels of total NEDD4-2.

背景

Nedd4-2 is a ubiquitin protein ligase of the Nedd4 family which is known to bind and regulate a number of membrane proteins to aid in their internalization and turnover. Several of the NEDD4-2 substrates include ion channels, such as the epithelial and voltage-gated sodium channels. NEDD4-2 plays critial roles in regulating membrane proteins and is essential for the maintenance of cellular homeostasis. Nedd4-2 is an E3 ubiquitin-protein ligase that reduces expression of the epithelial Na+ channel ENaC at the cell surface. Nedd4-2 catalyzes ubiquitination of α-, β-, and γENaC. Its overexpression increases ubiquitination, whereas Nedd4-2 silencing decreases ubiquitination.

使用信息

抗体应用

wb,ELISA,ELISA

稀释比例

WB

1:1000

反应性

Human Mouse Rat

MW (kDa)

130kDa

抗体类型

储存液配方

10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.

储存条件
(自收到货起)

Store at –20°C.

文献参考

Application Data

WB

Validated by Selleck