Recombinant Human CD72 Protein, CF (HEK293)

目录号：C0450

Recombinant Human CD72 Protein, CF (HEK293) is expressed from HEK293 cells with an His tag at the C-terminus.

生物描述

Accession

NP_001773.1, P21854

背景

B 细胞分化抗原 CD72 是单次跨膜 II 型膜蛋白。CD72 以二硫键连接的同源二聚体形式存在，含有一个 C 型凝集素结构域。CD72 表达于 B 谱系细胞、NK 细胞、单核细胞、树突状细胞和肥大细胞。CD72 是 CD5 的配体，可与 CD5 结合，同时结合 PTPN6/SHP‑1，参与 B 细胞的增殖与分化。抗原刺激后，CD72 可与 B 细胞抗原受体（BCR）复合物中的 CD79A 结合，并通过招募磷酸酶 SHP‑1 减弱 BCR 信号。

使用信息

物种

Human

来源

HEK293-drived Human CD72 Protein AA Arg 117 - Asp 359.

预测分子量

30.0 kDa

实际分子量

30-35 kDa, reducing conditions

纯度

>95% as determined by Bis-Tris PAGE.

内毒素

< 0.1 EU/ug by the LAL method

制剂

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

复溶方法

开盖前先10,000-12,000 rpm 离心 30 s；向冻干粉中加入无菌PBS，用枪头轻轻吹打混匀，重悬浓度不低于 100 μg/mL；复溶之后分装放-80℃保存，避免反复冻融，如要长期储存，建议添加保护剂（如0.1% BSA、5% HSA、10% FBS等）

储存条件 (自收到货起)

For long-term storage, keep the product lyophilized at -20°C or below. Please avoid repeated freeze-thaw cycles. Stability: Lyophilized: 12 months (-20°C to -80°C) Reconstituted: 3 months at -80°C under sterile conditions.

Data

: Recombinant Human CD72, CF (HEK293)on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.