Recombinant Human DR3/TNFRSF25 Protein, CF (HEK293)

目录号：C0446

Recombinant Human DR3/TNFRSF25 Protein, CF (HEK293) is expressed from HEK293 cells with an His tag at the C-terminus.

生物描述

Accession

Q93038-1

背景

DR3（TNFRSF25）是 TNFSF12/APO3L/TWEAK 的受体，可直接与衔接蛋白 TRADD 相互作用。该相互作用能够激活 NF‑κB 并诱导细胞凋亡，在淋巴细胞稳态调控中起到关键作用。DR3 可形成同源二聚体，其死亡结构域可与 TNFRSF1、TRADD 强相互作用，启动凋亡和 NF‑κB 信号通路两条独立的信号级联反应。此外，DR3/TNFRSF25 还可与 BAG4 结合，使其在细胞应答中具备多样功能。

使用信息

物种

Human

来源

HEK293-drived Human DR3/TNFRSF25 Protein AA Gln 25 - Gln 199.

预测分子量

20.9 kDa

实际分子量

30-37 kDa, reducing conditions

纯度

>95% as determined by Bis-Tris PAGE.

内毒素

< 0.1 EU/ug by the LAL method

制剂

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

复溶方法

开盖前先10,000-12,000 rpm 离心 30 s；向冻干粉中加入无菌PBS，用枪头轻轻吹打混匀，重悬浓度不低于 100 μg/mL；复溶之后分装放-80℃保存，避免反复冻融，如要长期储存，建议添加保护剂（如0.1% BSA、5% HSA、10% FBS等）

储存条件 (自收到货起)

For long-term storage, keep the product lyophilized at -20°C or below. Please avoid repeated freeze-thaw cycles. Stability: Lyophilized: 12 months (-20°C to -80°C) Reconstituted: 3 months at -80°C under sterile conditions.

Data

: Recombinant Human DR3/TNFRSF25 Protein, CF (HEK293)on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.