Recombinant Human ErbB2/Her2-Fc, CF(HEK293)

目录号：C0426

打印

Recombinant Human ErbB2/Her2-Fc Protein is expressed from HEK293 cells with an hFc and His tag at the C-terminus.

生物描述

Accession

P04626-1

别名

CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; NGL; TKR1; ERBB2; NEU; ENV; ENVW; ERVWE1; HERV7Q; HERVW

背景

ErbB2，亦称Neu和Her2（人表皮生长因子受体2），是一种I型膜糖蛋白，属于ErbB酪氨酸激酶受体家族。ErbB家族成员是表皮生长因子（EGF）家族生长因子的受体。ERBB2激活后，通过MEMO1-RHOA-DIAPH1信号通路引发细胞膜上GSK3B的磷酸化，从而抑制其活性。这阻止了APC和CLASP2的磷酸化，使二者得以结合于细胞膜。

使用信息

物种

Human

标签

hFc with His tag at the C-terminus.

来源

HEK293-drived Human ErbB2/Her2-Fc Protein AA Thr23-Thr652.

预测分子量

96.8 kDa

实际分子量

125-130 kDa, reducing conditions

纯度

>95% as determined by Bis-Tris PAGE.

内毒素

< 0.1 EU/ug by the LAL method

制剂

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

复溶方法

开盖前先10,000-12,000 rpm 离心 30 s；向冻干粉中加入无菌PBS，用枪头轻轻吹打混匀，重悬浓度低于 100 μg/mL；复溶之后分装放-80℃保存，避免反复冻融，如要长期储存，建议添加保护剂（如0.1% BSA、5% HSA、10% FBS等）

储存条件 (自收到货起)

For long-term storage, keep the product lyophilized at -20°C or below. Please avoid repeated freeze-thaw cycles. Stability: Lyophilized: 12 months (-20°C to -80°C) Reconstituted: 3 months at -80°C under sterile conditions.

Data

: Recombinant Human ErbB2/Her2-Fc, CF(HEK293)on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.