Recombinant Human HGF Protein, BSA (HEK293)

目录号：C0252

打印

Recombinant Human HGF Protein is expressed from HEK293 with His tag at the C-terminus.

生物描述

Accession
P14210.2
别名
HGF;DFNB39;F-TCF;HGFB;HPTA;SF
背景
肝细胞生长因子/分散因子 (HGF/SF)​ 是一种旁分泌细胞生长、运动和形态发生因子。它属于肽酶 S1 家族和纤溶酶原亚家族，包含 4 个 kringle 结构域、1 个 PAN 结构域和 1 个肽酶 S1 结构域。HGF 通过与原癌基因 c-Met 受体结合后激活酪氨酸激酶信号级联反应来调节细胞生长、细胞运动和形态发生。HGF 由间充质细胞分泌，并作为多功能细胞因子作用于主要上皮来源的细胞。其刺激有丝分裂、细胞运动和基质侵袭的能力使其在血管生成、肿瘤发生和组织再生中起核心作用。

使用信息

物种
Human
标签
His tag at the C-terminus.
来源
HEK293-drived human HGF Protein AA Gln 32-Ser 728.
预测分子量
81.6 kDa
实际分子量
The protein migrates as 80-90 kDa (alpha & Beta chain), 58-63 kDa (alpha chain), 33 kDa (Beta chain)
纯度
>95% as determined by Bis-Tris PAGE.
内毒素
< 0.1 EU/ug by the LAL method
制剂
Lyophilized from a 0.22 μm filtered solution in PBS with BSA as a carrier protein. Normally 8% trehalose is added as protectant before lyophilization.
复溶方法
开盖前先10,000-12,000 rpm 离心 30 s；向冻干粉中加入无菌PBS，用枪头轻轻吹打混匀，重悬浓度不低于 100 μg/mL；复溶之 后分装放-80℃保存，避免反复冻融。
储存条件 (自收到货起)
For long-term storage, keep the product lyophilized at -20°C or below. Please avoid repeated freeze-thaw cycles. Stability:​ Lyophilized: 12 months (-20°C to -80°C) Reconstituted: 3 months at -80°C under sterile conditions

Data

Quality Inspection Chart of Recombinant Human HGF Protein, BSA (HEK293)

Recombinant Human HGF Protein, BSA (HEK293)on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.