Recombinant Human IL-2 R beta / CD122 Protein, CF (HEK293)

目录号:C0451

打印

Recombinant Human IL-2 R beta / CD122 Protein, CF (HEK293) is expressed from HEK293 cells with an His tag at the C-terminus.

生物描述

Accession
NP_000869, P14784
背景
白介素‑2 受体 β 链(IL‑2 Rβ)属于细胞因子受体超家族。人源 IL‑2 Rβ 的 cDNA 编码含 551 个氨基酸残基的 I 型跨膜前体蛋白,包含 26 个残基的信号肽、214 个残基的胞外区、25 个残基的跨膜区以及 286 个残基的胞内结构域。细胞表面的功能性 IL‑2 受体存在两种亲和力形式:高亲和力复合物由 α、β、γ 链构成异源三聚体;中等亲和力复合物由 β、γ 链构成异源二聚体。

使用信息

物种
Human
来源
HEK293-drived Human IL-2 R beta / CD122 Protein AA Ala 27 - Asp 239.
预测分子量
25.4 kDa
实际分子量
35-45 kDa, reducing conditions
纯度
>95% as determined by Bis-Tris PAGE.
内毒素
< 0.1 EU/ug by the LAL method
制剂
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法
开盖前先10,000-12,000 rpm 离心 30 s;向冻干粉中加入无菌PBS,用枪头轻轻吹打混匀,重悬浓度不低于 100 μg/mL;复溶之后分装放-80℃保存,避免反复冻融,如要长期储存,建议添加保护剂(如0.1% BSA、5% HSA、10% FBS等)
储存条件 (自收到货起)
For long-term storage, keep the product lyophilized at -20°C or below. Please avoid repeated freeze-thaw cycles. Stability:​ Lyophilized: 12 months (-20°C to -80°C) Reconstituted: 3 months at -80°C under sterile conditions.

Data

Quality Inspection Chart of Recombinant Human IL-2 R beta / CD122 Protein, CF (HEK293)

Recombinant Human IL-2 R beta / CD122 Protein, CF (HEK293)on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.