Recombinant Human IL-3 Protein, BSA (HEK293)

目录号：C0008

打印

Recombinant Human IL-3 Protein is expressed from HEK293 with His tag at the C-terminus. It contains Ala20-Phe152.

生物描述

Accession
AAC08706
别名
IL3; IL-3; IL-3MGC79398; interleukin-3; MULTI-CSF; MCGF
背景
白细胞介素3是一种多效性因子，主要由活化的T细胞产生，能够刺激多能造血干细胞以及各谱系定向祖细胞的增殖和分化。

使用信息

物种
Human
标签
His tag at the C-terminus.
来源
HEK293-drived human IL-3 protein Ala20-Phe152.
活性
Recombinant Human IL-3 stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The EC50 for this effect is 0.3991-0.5613 ng/ml.
预测分子量
16.19 kDa
实际分子量
25-35 kDa, reducing conditions
纯度
>95% as determined by Bis-Tris PAGE.
内毒素
< 0.1 EU/ug by the LAL method
制剂
Lyophilized from 0.22μm filtered solution in PBS with BSA as a carrier protein. Normally 8% trehalose is added as protectant before lyophilization.
复溶方法
开盖前先10,000-12,000 rpm 离心 30 s；向冻干粉中加入无菌PBS，用枪头轻轻吹打混匀，重悬浓度不低于 100 μg/mL；复溶之 后分装放-80℃保存，避免反复冻融。
储存条件 (自收到货起)
For long-term storage, keep the product lyophilized at -20°C or below. Please avoid repeated freeze-thaw cycles. Stability:​ Lyophilized: 12 months (-20°C to -80°C) Reconstituted: 3 months at -80°C under sterile conditions

Data

Quality Inspection Chart of Recombinant Human IL-3 Protein, BSA (HEK293)

Recombinant Human IL-3 Protein (HEK293) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Quality Inspection Chart of Recombinant Human IL-3 Protein, BSA (HEK293)

Recombinant Human IL-3 stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The EC50 for this effect is 0.3991-0.5613 ng/ml.