Recombinant Human Kallikrein 5/KLK5 Protein , C-His, CF (HEK293)

目录号:C0445

打印

Recombinant Human Kallikrein 5/KLK5 Protein, CF (HEK293) is expressed from HEK293 cells with an His tag at the C-terminus.

生物描述

Accession
Q9Y337
别名
KLK-L2, KLK5, SCTE
背景
人组织激肽释放酶 5(hK5,又称角质层胰蛋白酶)是人组织激肽释放酶基因家族的丝氨酸蛋白酶。人 KLK5 推导氨基酸序列包含信号肽(Met1~Gly22)、前肽区(Val23~Arg66)以及成熟活性酶区(Ile67~Ser293)。KLK5 主要在皮肤、乳腺、脑和睾丸中表达,在其他多种组织中低水平表达。KLK5 过表达是卵巢癌预后不良的标志物。

使用信息

物种
Human
标签
His tag at the C-terminus.
来源
HEK293-drived Human Kallikrein 5/KLK5 Protein AA Ile67-Ser293.
预测分子量
26.5 kDa
实际分子量
35-50 kDa, reducing conditions
纯度
>95% as determined by Bis-Tris PAGE.
内毒素
< 0.1 EU/ug by the LAL method
制剂
Lyophilized from 0.22 μm filtered solution in 50mM MES, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法
开盖前先10,000-12,000 rpm 离心 30 s;向冻干粉中加入无菌PBS,用枪头轻轻吹打混匀,重悬浓度不低于 100 μg/mL;复溶之后分装放-80℃保存,避免反复冻融,如要长期储存,建议添加保护剂(如0.1% BSA、5% HSA、10% FBS等)
储存条件 (自收到货起)
For long-term storage, keep the product lyophilized at -20°C or below. Please avoid repeated freeze-thaw cycles. Stability:​ Lyophilized: 12 months (-20°C to -80°C) Reconstituted: 3 months at -80°C under sterile conditions.

Data

Quality Inspection Chart of Recombinant Human Kallikrein 5/KLK5 Protein , C-His, CF (HEK293)

Recombinant Human Kallikrein 5/KLK5 Protein , C-His, CF (HEK293) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.