Recombinant Human Transferrin R / CD71 Protein, CF (HEK293)

目录号：C0449

Recombinant Human Transferrin R / CD71 Protein, CF (HEK293) is expressed from HEK293 cells with an His tag at the N-terminus.

生物描述

Accession

P02786-1

背景

转铁蛋白受体（CD71）是系膜细胞上 IgA1 的受体。研究采用实时荧光定量 PCR 与免疫组化检测肾小球 CD71 的转录水平与蛋白表达；将人系膜细胞（HMC）用 IgA 肾病患者血清处理后，根据疾病状态对比 CD71 及炎症细胞因子标志物的表达水平。

使用信息

物种

Human

来源

HEK293-drived Human Transferrin R / CD71 Protein AA Cys 89 - Phe 760.

预测分子量

77.0 kDa

实际分子量

80 kDa, reducing conditions

纯度

>95% as determined by Bis-Tris PAGE.

内毒素

< 0.1 EU/ug by the LAL method

制剂

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

复溶方法

开盖前先10,000-12,000 rpm 离心 30 s；向冻干粉中加入无菌PBS，用枪头轻轻吹打混匀，重悬浓度不低于 100 μg/mL；复溶之后分装放-80℃保存，避免反复冻融，如要长期储存，建议添加保护剂（如0.1% BSA、5% HSA、10% FBS等）

储存条件 (自收到货起)

For long-term storage, keep the product lyophilized at -20°C or below. Please avoid repeated freeze-thaw cycles. Stability: Lyophilized: 12 months (-20°C to -80°C) Reconstituted: 3 months at -80°C under sterile conditions.

Data

: Recombinant Human Transferrin R / CD71 Protein, CF (HEK293)on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.