Recombinant Human TrkB Fc, CF(HEK293)

目录号：C0427

打印

Recombinant Human TrkB Fc Protein is expressed from HEK293 cells with an hFc and His tag at the C-terminus.

生物描述

Accession
Q16620, NP_001018074.1
别名
NTRK2; GP145-TrkB; TRKB; trk-B; BDNF/NT-3 growth factors receptor;GP145-TrkB;TRKB;trkB;TrkB;OBHD
背景
TrkB受体，又称TrkB酪氨酸激酶或BDNF/NT-3生长因子受体或神经营养性酪氨酸激酶受体2型（NTRK2），是一种具有胞内酪氨酸激酶活性的单次跨膜催化受体。TrkB/NTRK2属于神经营养性酪氨酸受体激酶（NTRK）家族，包含两个Ig样C2型结构域、两个LRR（富含亮氨酸）重复序列、一个LRRCT结构域、一个LRRNT结构域及一个蛋白激酶结构域。TrkB/NTRK2酪氨酸激酶与Ras、Cdc42/Rac/RhoG、MAPK、PI3-K和PLCγ信号通路相偶联。TrkB/NTRK对脑源性神经营养因子（BDNF）具有高亲和力，参与神经元可塑性、长时程增强及中枢神经系统神经元的凋亡过程。作为膜结合受体，TrkB/NTRK与神经营养因子结合后发生自磷酸化，并磷酸化MAPK通路成员。NTRK2/TrkB通过调控神经元存活、增殖、迁移、分化及突触形成与可塑性，参与中枢与周围神经系统的发育和成熟。TrkB/NTRK的突变与肥胖及情绪障碍相关。

使用信息

物种
Human
标签
hFc with His tag at the C-terminus.
来源
HEK293-drived Human TrkB Fc Protein AA Cys32-His430.
预测分子量
71.7 kDa
实际分子量
120 kDa-130 kDa, reducing conditions
纯度
>95% as determined by Bis-Tris PAGE.
内毒素
< 0.1 EU/ug by the LAL method
制剂
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法
开盖前先10,000-12,000 rpm 离心 30 s；向冻干粉中加入无菌PBS，用枪头轻轻吹打混匀，重悬浓度低于 100 μg/mL；复溶之后分装放-80℃保存，避免反复冻融，如要长期储存，建议添加保护剂（如0.1% BSA、5% HSA、10% FBS等）
储存条件 (自收到货起)
For long-term storage, keep the product lyophilized at -20°C or below. Please avoid repeated freeze-thaw cycles. Stability:​ Lyophilized: 12 months (-20°C to -80°C) Reconstituted: 3 months at -80°C under sterile conditions.

Data

Quality Inspection Chart of Recombinant Human TrkB Fc, CF(HEK293)

Recombinant Human TrkB Fc, CF(HEK293)on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.