Recombinant Mouse CD14 Protein, CF (HEK293)

目录号：C0251

打印

Recombinant Mouse CD14 Protein is expressed from HEK293 with His tag at the C-terminus.

生物描述

Accession
P10810
别名
CD14; CD14 molecule
背景
CD14​ 是一种 GPI 锚定糖蛋白，在成熟单核细胞、巨噬细胞和中性粒细胞表面组成型表达。它作为一种多功能脂多糖 (LPS) 受体，并以分泌型和酶切 GPI 锚定形式释放到血清中。CD14 结合 LPS，该反应由脂多糖结合蛋白 (LBP)（一种急性期血清蛋白）促进。可溶性形式 sCD14 可以区分 LPS 分子之间的细微结构差异，并在通过重构脂蛋白颗粒中和血清脂多糖中起关键作用。CD14 也结合凋亡细胞并影响过敏、炎症和感染过程。可变剪接产生编码相同 CD14 亚型的多种转录变体。CD14 的功能障碍与疾病有关，如龟分枝杆菌感染和哮吼。

使用信息

物种
Mouse
标签
His tag at the C-terminus.
来源
HEK293-drived mouse CD14 Protein AA Ser16-Pro345.
预测分子量
38.19 kDa
实际分子量
52-62 kDa , reducing conditions
纯度
>95% as determined by Bis-Tris PAGE.
内毒素
< 0.1 EU/ug by the LAL method
制剂
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法
开盖前先10,000-12,000 rpm 离心 30 s；向冻干粉中加入无菌PBS，用枪头轻轻吹打混匀，重悬浓度低于 100 μg/mL；复溶之后分装放-80℃保存，避免反复冻融，如要长期储存，建议添加保护剂（如0.1% BSA、5% HSA、10% FBS等）
储存条件 (自收到货起)
For long-term storage, keep the product lyophilized at -20°C or below. Please avoid repeated freeze-thaw cycles. Stability:​ Lyophilized: 12 months (-20°C to -80°C) Reconstituted: 3 months at -80°C under sterile conditions

Data

Quality Inspection Chart of Recombinant Mouse CD14 Protein, CF (HEK293)

Recombinant Mouse CD14 Protein, CF (HEK293)on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.