Recombinant Mouse IL-5 Protein, BSA (HEK293)

目录号：C0014

打印

Recombinant Mouse IL-5 Protein is expressed from HEK293 with His tag at the C-terminus. It contains Met21-Gly133.

生物描述

Accession

P04401-1

别名

IL-5, TRF, IL5, Interleukin-5

背景

白介素 5 (IL5) 是由 2 型 T 辅助细胞和肥大细胞产生的白介素。IL-5 是一种长 115 个氨基酸（在人类中为 115 个氨基酸，在小鼠中为 133 个氨基酸）的 TH2 细胞因子，属于造血细胞因子家族。与该细胞因子家族的其他成员（即白介素 3 和 GM-CSF）不同，这种糖蛋白的活性形式为同型二聚体。长期以来，白介素 5 一直与多种过敏性疾病的病因相关，包括过敏性鼻炎和哮喘，这些疾病中已观察到循环、气道组织和诱导痰中嗜酸性粒细胞数量大幅增加。鉴于嗜酸性粒细胞（尤其是过敏性哮喘病理）的高度一致性，人们普遍推测嗜酸性粒细胞在该疾病的病理学中起着重要作用。靶向 IL-5 的药物有美泊利单抗和瑞利珠单抗。

使用信息

物种

Mouse

标签

His tag at the N-terminus.

来源

HEK293-drived mouse IL-6 protein Met21-Gly133.

预测分子量

13.1 kDa

实际分子量

15-21 kDa, reducing conditions

纯度

>95% as determined by Bis-Tris PAGE.

内毒素

< 0.1 EU/ug by the LAL method

制剂

Lyophilized from 0.22μm filtered solution in PBS with BSA as a carrier protein. Normally 8% trehalose is added as protectant before lyophilization.

复溶方法

开盖前先10,000-12,000 rpm 离心 30 s；向冻干粉中加入无菌PBS，用枪头轻轻吹打混匀，重悬浓度不低于 100 μg/mL；复溶之后分装放-80℃保存，避免反复冻融。

储存条件 (自收到货起)

For long-term storage, keep the product lyophilized at -20°C or below. Please avoid repeated freeze-thaw cycles. Stability: Lyophilized: 12 months (-20°C to -80°C) Reconstituted: 3 months at -80°C under sterile conditions

Data

: Recombinant Mouse IL-5 Protein (HEK293) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

: Recombinant Mouse IL-5 stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The EC50 for this effect is 0.2935 ng/ml.