Recombinant Mouse Noggin Protein, CF (HEK293)

目录号：C0154

打印

Recombinant Mouse Noggin Protein is expressed from HEK293 with His tag at the C-Terminus.

生物描述

Accession
P97466
别名
Noggin, NOG, SYM1, SYNS1
背景
Noggin​ 是骨形态发生蛋白 (BMP) 的拮抗剂，对于某些发育事件是不可或缺的。Noggin 表达在 GC 细胞系模型和癌症基因组图谱人类 GC 队列中均与 EGFR 表达呈正相关。靶向 EGFR 及其下游通路可减少由 Noggin 促进的细胞增殖。Noggin 通过上调 EGFR 和增强由 β-连环蛋白、EGFR、ERK 和 Akt 形成的恶性循环来促进 GC 细胞的增殖。

使用信息

物种
Mouse
标签
His tag at the C-terminus.
来源
HEK293-drived mosue Noggin protein AA Gln28-Cys232.
预测分子量
25.7 kDa
实际分子量
28-38 kDa , reducing conditions
纯度
>95% as determined by Bis-Tris PAGE.
内毒素
< 0.1 EU/ug by the LAL method
制剂
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法
开盖前先10,000-12,000 rpm 离心 30 s；向冻干粉中加入无菌PBS，用枪头轻轻吹打混匀，重悬浓度低于 100 μg/mL；复溶之后分装放-80℃保存，避免反复冻融，如要长期储存，建议添加保护剂（如0.1% BSA、5% HSA、10% FBS等）
储存条件 (自收到货起)
For long-term storage, keep the product lyophilized at -20°C or below. Please avoid repeated freeze-thaw cycles. Stability:​ Lyophilized: 12 months (-20°C to -80°C) Reconstituted: 3 months at -80°C under sterile conditions

Data

Quality Inspection Chart of Recombinant Mouse Noggin Protein, CF (HEK293)

Recombinant Mouse Noggin Protein, CF (HEK293)on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.