Caffeic Acid Phenethyl Ester

别名: CAPE, Phenylethyl Caffeate 中文名称：咖啡酸苯乙酯

目录号：S7414 Purity: 99.99%

Caffeic acid phenethyl ester (CAPE, Phenylethyl Caffeate)是强效的特异性NF-κB活化抑制剂，也能够表现出抗氧化、免疫调节和抗炎活动。

Caffeic Acid Phenethyl Ester Chemical Structure

CAS: 104594-70-9

规格	价格	库存
50mg	808.46	现货
200mg	2213.96	现货
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产品质控

批次：纯度： 99.99%

99.99

Caffeic Acid Phenethyl Ester相关产品

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细胞实验数据示例

细胞系	实验类型	给药浓度	孵育时间	活性描述	文献信息
rat VSMC	Function assay	5 μM	30 mins	Decrease in intracellular ROS level in PDGF-stimulated rat VSMC at 5 uM pretreated with compound for 30 mins measured after up to 23 hrs of post stimulation using H2DCF by flow cytometric analysis	21265554
rat PC12 cells	Function assay	2.5 μM	3 h	Neuroprotective activity against H2O2-induced damage in rat PC12 cells assessed as cell viability at 2.5 uM preincubated for 3 hrs followed by H2O2 challenge measured after 5 hrs by MTT assay	24697335
mouse RAW264.7 cells	Function assay		24 h	Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation administered 1 hr before LPS challenge and measured after 24 hrs by Griess reagent assay, EC50=0.193 μM	18667320
mouse 26-L5 cells	Proliferation assay		72 h	Antiproliferative activity against mouse 26-L5 cells after 72 hrs by MTT assay, EC50=0.3 μM	12027739
human HeLa cells	Proliferation assay		72 h	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay, EC50=2.36 μM	12027739
mouse J774.1 cells	Function assay		24 h	Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs by Griess reagent assay, IC50=4.8 μM	15974608
human LNCAP cells	Function assay		24 h	Antiandrogenic activity in human LNCAP cells assessed as reduction in DHT-stimulated PSA release after 24 hrs by ELISA, IC50=6.2 nM	24080105
mouse BV2 cells	Function assay		24 h	Inhibition of nitric oxide production in LPS-stimulated mouse BV2 cells treated 3 hrs before LPS addition measured after 24 hrs by Griess assay, IC50=6.4 μM	23870700
mouse B16-BL6 cells	Proliferation assay		72 h	Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay, EC50=6.79 μM	12027739
HUVEC cells	Cytotoxic assay		18 h	Cytoprotective activity against H2O2-induced oxidative stress in HUVEC cells after 18 hrs by Cell-Titer Blue assay	20598894
human HT1080 cells	Proliferation assay		72 h	Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay, EC50=9.5 μM	12027739
human HL60 cells	Cytotoxic assay		72 h	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay, IC50=9.8 μM	20673727
human LS 174T cells	Cytotoxic assay		72 h	Cytotoxicity against human LS 174T cells after 72 hrs by MTT assay, IC60=9.9 μM	20673727
human Bel7404 cells	Cytotoxic assay		72 h	Cytotoxicity against human Bel7404 cells after 72 hrs by MTT assay, IC50=14.5 μM	20673727
human BGC823 cells	Cytotoxic assay		72 h	Cytotoxicity against human BGC823 cells after 72 hrs by MTT assay, IC50=19.3 μM	20673727
human HO8910 cells	Cytotoxic assay		72 h	Cytotoxicity against human HO8910 cells after 72 hrs by MTT assay, IC50=25.5 μM	20673727
human A549 cells	Cytotoxic assay		72 h	Cytotoxicity against human A549 cells after 72 hrs by MTT assay, IC50=26.8 μM	20673727
human LNCAP cells	Cytotoxic assay		24 h	Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 24 hrs by WST-1 assay, IC50=33.7 μM	24080105
human ECA109 cells	Cytotoxic assay		72 h	Cytotoxicity against human ECA109 cells after 72 hrs by MTT assay, IC50=42.6 μM	20673727
human KB cells	Cytotoxic assay		72 h	Cytotoxicity against human KB cells after 72 hrs by MTT assay, IC50=45.2 μM	20673727
human K562 cells	Cytotoxic assay		72 h	Cytotoxicity against human K562 cells after 72 hrs by MTT assay, IC50=46 μM	20673727
human Bewo cells	Function assay			Antitumor activity against human Bewo cells assessed as inhibition of cell growth by MTT assay, IC50=2.96 μM	25160837
human Bel7402 cells	Cytotoxic assay			Cytotoxicity against human Bel7402 cells by MTT assay, IC50=5.5 μM	18952420
human SGC7901 cells	Function assay			Antitumor activity against human SGC7901 cells assessed as inhibition of cell growth by MTT assay, IC50=14.26 μM	25160837
human MCF7 cells	Cytotoxic assay			Cytotoxicity against human MCF7 cells by MTT assay, IC50=26.7 μM	18952420
human HeLa cells	Function assay			Antitumor activity against human HeLa cells assessed as inhibition of cell growth by MTT assay, IC50=37.98 μM	25160837
human HepG2 cells	Function assay			Antitumor activity against human HepG2 cells assessed as inhibition of cell growth by MTT assay, IC50=40.87 μM	25160837
human BCG823 cells	Cytotoxic assay			Cytotoxicity against human BCG823 cells by MTT assay, IC50=44.6 μM	18952420
点击查看更多细胞系数据

生物活性

产品描述

Caffeic acid phenethyl ester (CAPE, Phenylethyl Caffeate)是强效的特异性NF-κB活化抑制剂，也能够表现出抗氧化、免疫调节和抗炎活动。

靶点

NF-κB ^[1]

体外研究(In Vitro)
体外研究活性	Caffeic acid phenethyl ester能够通过抑制p65的入核转运来抑制佛波酯，神经酰胺，强心酸过氧化氢等诱导的NF-kB的激活。^[1] 在一系列的肿瘤细胞系中，Caffeic acid phenethyl ester具有抗增殖的活性，其对26-L5, B16-BL6恶性瘤, 人类HT-1080的 EC50 分别是 1.76, 3.16, 13.7,和44.0 μM。^[2] Caffeic acid phenethyl ester通过抑制ROS的形成和抑制半胱氨酸天冬氨酸蛋白酶的活性发挥抗凋亡的作用。Caffeic acid phenethyl ester通过抑制NF-kB信号通路来减弱LPS诱导的炎症和LPS诱导的HSCs细胞对细胞因子的敏感性。^[4]
细胞实验	细胞系	小鼠结肠 26-L5, 小鼠B16-BL6黑色素瘤,人类HT-1080 纤维肉瘤和人类肺 A549 腺癌细胞系。
	浓度	~200 μM
	孵育时间	3天
	方法	人HT-1080纤维肉瘤，人肺癌A549腺癌细胞和鼠B16-BL6黑色素瘤细胞在含有10%的血清，0.1%碳酸盐，和2mM的谷氨酰胺的EMEM培养基中培养。鼠结肠癌细胞 B16-BL6用含有相同添加成分的RPMI中培养。除了A-549以外，这些都是有高度侵染能力的细胞系。细胞存活实验采用标准的MTT方法。简单的说，对数生长的细胞收集以后以2000个每孔的密度种植于96孔板。24小时孵育后，用含有不同浓度的样品的100ul培养基处理细胞，5%的二氧化碳37度条件下培养72小时。加入MTT3小时以后用Perkin Elmer HTS-7000测定550纳米波长的吸收值。待测样品先用DMSO溶解，再用培养基稀释。DMSO的终浓度小于0.25%。对照组还有相同浓度的DMSO。5-氟尿嘧啶(5-FU) 和 doxorubicin HCl用作阳性对照。EC50值通过取4个孔数据的平均值来计算的。
实验图片	检测方法	检测指标	实验图片	PMID
	Western blot	p-p65 / CDH6 / RUNX2 / E-cadherin / N-cadherin / Vimentin / Slug / Snail Nrf2 AKT / AKT1 / AKT2 / AKT3 / p-AKT / FOXO1 / FOXO3a / p-FOXO1 / p-FOXO3a / Skp2 / p27(Kip) / Cyclin D1 / Rb / p-Rb		29284791
	Immunofluorescence	NF-κB		29284791
	Growth inhibition assay	Cell number		23615471

体内研究(In Vivo)
体内研究活性	C57BL/6 and BALB/c小鼠腹腔内注射10毫克/千克Caffeic acid phenethyl ester能够抑制在路易斯肺细胞癌，结肠癌，和黑色素瘤的生长和肿瘤的血管新生。^[5]在动物体内(5, 10, 20 mg/kg) 的Caffeic acid phenethyl ester通过减少胸腺的重量和胸腺和脾的孔性而具有免疫调节的作用。^[6]
动物实验	Animal Models	接种Lewis肺肿瘤，结肠癌以及黑色素瘤细胞的C57BL/6 和 BALB/c 小鼠
	Dosages	~10毫克/千克
	Administration	腹腔注射

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
临床试验信息 (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT05726708	Recruiting	Autism Spectrum Disorder	Centre Hospitalier Universitaire Vaudois	October 24 2023	Not Applicable
NCT05934019	Recruiting	Mental Health Issue\|Transdiagnostic Mechanisms\|Adolescent Psychology\|Prevention\|Online Intervention\|Psychological Intervention\|Mental Disorder in Adolescence Subclinical	University of Bern	June 9 2023	Not Applicable

参考文献
[1] Natarajan K, et al. Proc Natl Acad Sci U S A. 1996, 93(17), 9090-9095. [2] Banskota AH, et al. J Ethnopharmacol. 2002, 80(1), 67-73. [3] Amodio R, et al. Int J Dev Neurosci. 2003, 21(7), 379-389. [4] Zhao WX, et al. Int J Mol Med. 2014, 33(3), 687-694. [5] Chung TW, et al. J Mol Med (Berl). 2013, 91(2), 271-282. [6] Park JH, et al. Int Immunopharmacol. 2004, 4(3), 429-436. + 展开

参考文献

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化学信息&溶解度

分子量	284.31	分子式	C₁₇H₁₆O₄
CAS号	104594-70-9	SDF	Download Caffeic Acid Phenethyl Ester SDF
Smiles	C1=CC=C(C=C1)CCOC(=O)C=CC2=CC(=C(C=C2)O)O
储存条件(自收到货起)	3年-20°C粉状	储备液保存和期限建议分装储备液，避免反复冻融！	1年-80°C溶于溶剂
储存条件(自收到货起)	3年-20°C粉状	储备液保存和期限建议分装储备液，避免反复冻融！	1个月-20°C溶于溶剂

体外溶解度
批次：

DMSO : 56 mg/mL ( (196.96 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Ethanol : 56 mg/mL (196.96 mM)

Water : Insoluble

DMSO : 57 mg/mL ( (200.48 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Ethanol : 57 mg/mL (200.48 mM)

Water : Insoluble

DMSO : 57 mg/mL ( (200.48 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Ethanol : 57 mg/mL (200.48 mM)

Water : Insoluble

摩尔浓度计算器

体内溶解度
批次：

现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂

动物体内配方计算器

实验计算

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×

稀释计算器分子量计算器

动物体内配方计算器（澄清溶液）

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 μL 动物数量只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

计算结果：

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH₂O，混匀澄清。

体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。

注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

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Caffeic Acid Phenethyl Ester

产品质控

Caffeic Acid Phenethyl Ester相关产品

相关信号通路图

细胞实验数据示例

生物活性

化学信息&溶解度

实验计算

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