Isoxazole 9 (ISX-9)

Isoxazole 9 (Isx-9)是一种成体神经发生的合成促进剂,通过引起成熟神经干细胞/前体细胞(NSPCs)的神经元分化而发挥作用。Isoxazole 9 (Isx-9) 可激活多条信号通路,包括 TGF-β 诱导的上皮-间质转化信号通路 (EMT) ,以及在心脏分化不同阶段的经典和非经典的 Wnt 信号通路。

Isoxazole 9 (ISX-9) Chemical Structure

Isoxazole 9 (ISX-9) Chemical Structure

CAS: 832115-62-5

规格 价格 库存 购买数量
10mM (1mL in DMSO) 1270 现货
5mg 961.69 现货
25mg 2835.03 现货
100mg 6530.16 现货
1g 24324.3 现货
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Isoxazole 9 (ISX-9)相关产品

相关信号通路图

细胞实验数据示例

细胞系 实验类型 给药浓度 孵育时间 活性描述 文献信息
HCN Function assay 50 uM 3 hrs Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 3 hrs by RT-PCR analysis in presence of NMDA receptor antagonist MK801 18552832
HCN Function assay 25 uM 4 days Inhibition of gliogenesis differentiation in rat HCN cells at 25 uM after 4 days 18552832
HCN Function assay 50 uM 3 hrs Induction of L-type calcium channel/NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 3 hrs by RT-PCR analysis in presence of multiple inhibitors 18552832
HCN Function assay 50 uM 24 hrs Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 24 hrs by RT-PCR analysis in presence of NMDA receptor antagonist MK801 18552832
HCN Function assay 50 uM 24 hrs Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 24 hrs by RT-PCR analysis in presence of NMDA receptor antagonist nifedipine 18552832
HCN Function assay 50 uM 24 hrs Induction of L-type calcium channel/NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 24 hrs by RT-PCR analysis in presence of multiple inhibitors 18552832
HCN Function assay 8 to 64 uM 9 hrs Induction of neurogenesis in undifferentiated rat HCN cells assessed as MAP2AB protein level at 8 to 64 uM after 9 hrs by protein blotting analysis 18552832
HCN Function assay 8 to 64 uM 9 hrs Induction of neurogenesis in undifferentiated rat HCN cells assessed as GlR2/3 protein level at 8 to 64 uM after 9 hrs by protein blotting analysis 18552832
HCN Function assay 50 uM 3 hrs Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 3 hrs by RT-PCR analysis in presence of NMDA receptor antagonist nifedipine 18552832
HCN Function assay 5 uM Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells at 5 uM by Fura-2 imaging analysis in presence of multiple of inhibitors 18552832
HCN Function assay 5 uM Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells at 5 uM by Fura-2 imaging analysis 18552832
HCN Function assay 5 uM Induction of NMDA receptor-mediated Ca2+ influx in rat HCN cells at 5 uM by Fura-2 imaging analysis in presence of NMDA receptor antagonist MK801 18552832
HCN Function assay 5 uM Induction of HDAC5 translocation in nucleus of rat HCN cells assessed as GFP-HDAC5 S258A S498A mutant fusion protein accumulation at 5 uM by fluorescence assay 18552832
HCN Function assay 20 uM Induction of HDAC5 translocation in nucleus of rat HCN cells assessed as GFP-HDAC5 fusion protein accumulation at 20 uM by fluorescence assay 18552832
HCN Function assay 6 hrs Induction of HDAC5 phosphorylation in rat HCN cells after 6 hrs 18552832
HCN Function assay 24 hrs Induction of HDAC5 phosphorylation in rat HCN cells after 24 hrs 18552832
HCN Function assay 24 hrs Induction of L-type calcium channel-mediated neuroD gene expression in rat HCN cells after 24 hrs by luciferase reporter gene assay in presence of multiple inhibitors 18552832
HCN Function assay 24 hrs Induction of L-type calcium channel-mediated neuroD gene expression in rat HCN cells after 24 hrs by luciferase reporter gene assay in presence of NMDA receptor antagonist MK801 18552832
HCN Function assay 24 hrs Induction of L-type calcium channel-mediated neuroD gene expression in rat HCN cells after 24 hrs by luciferase reporter gene assay in presence of NMDA receptor antagonist nifedipine 18552832
HCN Function assay Induction of NMDA receptor-mediated Ca2+ influx in rat HCN cells in presence of NMDA receptor antagonist MK801 18552832
HCN Function assay Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells 18552832
HCN Function assay Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells in presence multiple inhibitors 18552832
HCN Function assay Induction of HDAC5 translocation in cytoplasm of rat HCN cells assessed as phosphorylated HDAC5 accumulation 18552832
HCN Function assay Induction of CAMK-mediated MREx3 activity in rat HCN cells by luciferase reporter gene assay in presence of 2.5 uM potassium channel inhibitor KN92 18552832
HCN Function assay Induction of HDAC5 translocation in nucleus of rat HCN cells assessed as phosphorylated HDAC5 accumulation 18552832
HCN Function assay Induction of CAMK-mediated MREx3 activity in rat HCN cells by luciferase reporter gene assay in presence of 200 nM PKC inhibitor Go6976 18552832
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生物活性

产品描述 Isoxazole 9 (Isx-9)是一种成体神经发生的合成促进剂,通过引起成熟神经干细胞/前体细胞(NSPCs)的神经元分化而发挥作用。Isoxazole 9 (Isx-9) 可激活多条信号通路,包括 TGF-β 诱导的上皮-间质转化信号通路 (EMT) ,以及在心脏分化不同阶段的经典和非经典的 Wnt 信号通路。
体外研究(In Vitro)
体外研究活性 在NSPCs中,isoxazole 9增加细胞数量,并促进细胞分化。在OPCs中,isoxazole 9诱导细胞损伤。在生长的EPCs中,isoxazole 9减少微管形成,而不影响早期EPCs。[1]
细胞实验 细胞系 NSPCs,OPCs,早期EPCs,和副产物EPCs
浓度 ~50 μM
孵育时间 48小时
方法 细胞用不同浓度的Isx-9 (0,6.25,12.5,25,或50 μM)处理,2天后,细胞数量以盲方式计数,以评估Isx-9对四种类型祖细胞/前体细胞的增殖/存活的作用。
体内研究(In Vivo)
体内研究活性 在小鼠中,isoxazole 9 (20 mg/kg, i.p.)穿过血脑屏障,并通过Mef2-特异性机制,增加海马体SGZ中神经干细胞的增殖和神经发生。 Isoxazole 9 (20 mg/kg, i.p.)也会增加不成熟神经元的分化和树突复杂度,并改善记忆。在MWM中,isoxazole 9改善空间记忆。[2]
动物实验 Animal Models 转基因Nkx2.5-荧光素酶报告基因小鼠
Dosages 20 mg/kg
Administration i.p.

化学信息&溶解度

分子量 234.27 分子式

C11H10N2O2S

CAS号 832115-62-5 SDF Download Isoxazole 9 (ISX-9) SDF
Smiles C1CC1NC(=O)C2=NOC(=C2)C3=CC=CS3
储存条件(自收到货起)

体外溶解度
批次:

DMSO : 47 mg/mL ( (200.62 mM) ;DMSO吸湿会降低化合物溶解度,请使用新开封DMSO)

Ethanol : 12 mg/mL (51.22 mM)

Water : Insoluble

摩尔浓度计算器

体内溶解度
批次:

现配现用,请按从左到右的顺序依次添加,澄清后再加入下一溶剂

动物体内配方计算器

实验计算

摩尔浓度计算器

质量 浓度 体积 分子量

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

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