Isoxazole 9 (ISX-9)
Isoxazole 9 (Isx-9)是一种成体神经发生的合成促进剂，通过引起成熟神经干细胞/前体细胞(NSPCs)的神经元分化而发挥作用。Isoxazole 9 (Isx-9) 可激活多条信号通路，包括 TGF-β 诱导的上皮-间质转化信号通路 (EMT) ，以及在心脏分化不同阶段的经典和非经典的 Wnt 信号通路。
Isoxazole 9 (ISX-9) Chemical Structure
CAS: 832115-62-5
产品质控
批次：
纯度：
99.87%
99.87
Isoxazole 9 (ISX-9)相关产品
相关信号通路图
细胞实验数据示例
| 细胞系 | 实验类型 | 给药浓度 | 孵育时间 | 活性描述 | 文献信息 |
|---|---|---|---|---|---|
| HCN | Function assay | 50 uM | 3 hrs | Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 3 hrs by RT-PCR analysis in presence of NMDA receptor antagonist MK801 | 18552832 |
| HCN | Function assay | 25 uM | 4 days | Inhibition of gliogenesis differentiation in rat HCN cells at 25 uM after 4 days | 18552832 |
| HCN | Function assay | 50 uM | 3 hrs | Induction of L-type calcium channel/NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 3 hrs by RT-PCR analysis in presence of multiple inhibitors | 18552832 |
| HCN | Function assay | 50 uM | 24 hrs | Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 24 hrs by RT-PCR analysis in presence of NMDA receptor antagonist MK801 | 18552832 |
| HCN | Function assay | 50 uM | 24 hrs | Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 24 hrs by RT-PCR analysis in presence of NMDA receptor antagonist nifedipine | 18552832 |
| HCN | Function assay | 50 uM | 24 hrs | Induction of L-type calcium channel/NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 24 hrs by RT-PCR analysis in presence of multiple inhibitors | 18552832 |
| HCN | Function assay | 8 to 64 uM | 9 hrs | Induction of neurogenesis in undifferentiated rat HCN cells assessed as MAP2AB protein level at 8 to 64 uM after 9 hrs by protein blotting analysis | 18552832 |
| HCN | Function assay | 8 to 64 uM | 9 hrs | Induction of neurogenesis in undifferentiated rat HCN cells assessed as GlR2/3 protein level at 8 to 64 uM after 9 hrs by protein blotting analysis | 18552832 |
| HCN | Function assay | 50 uM | 3 hrs | Induction of NMDA receptor-mediated neuroD gene expression in rat HCN cells at 50 uM after 3 hrs by RT-PCR analysis in presence of NMDA receptor antagonist nifedipine | 18552832 |
| HCN | Function assay | 5 uM | Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells at 5 uM by Fura-2 imaging analysis in presence of multiple of inhibitors | 18552832 | |
| HCN | Function assay | 5 uM | Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells at 5 uM by Fura-2 imaging analysis | 18552832 | |
| HCN | Function assay | 5 uM | Induction of NMDA receptor-mediated Ca2+ influx in rat HCN cells at 5 uM by Fura-2 imaging analysis in presence of NMDA receptor antagonist MK801 | 18552832 | |
| HCN | Function assay | 5 uM | Induction of HDAC5 translocation in nucleus of rat HCN cells assessed as GFP-HDAC5 S258A S498A mutant fusion protein accumulation at 5 uM by fluorescence assay | 18552832 | |
| HCN | Function assay | 20 uM | Induction of HDAC5 translocation in nucleus of rat HCN cells assessed as GFP-HDAC5 fusion protein accumulation at 20 uM by fluorescence assay | 18552832 | |
| HCN | Function assay | 6 hrs | Induction of HDAC5 phosphorylation in rat HCN cells after 6 hrs | 18552832 | |
| HCN | Function assay | 24 hrs | Induction of HDAC5 phosphorylation in rat HCN cells after 24 hrs | 18552832 | |
| HCN | Function assay | 24 hrs | Induction of L-type calcium channel-mediated neuroD gene expression in rat HCN cells after 24 hrs by luciferase reporter gene assay in presence of multiple inhibitors | 18552832 | |
| HCN | Function assay | 24 hrs | Induction of L-type calcium channel-mediated neuroD gene expression in rat HCN cells after 24 hrs by luciferase reporter gene assay in presence of NMDA receptor antagonist MK801 | 18552832 | |
| HCN | Function assay | 24 hrs | Induction of L-type calcium channel-mediated neuroD gene expression in rat HCN cells after 24 hrs by luciferase reporter gene assay in presence of NMDA receptor antagonist nifedipine | 18552832 | |
| HCN | Function assay | Induction of NMDA receptor-mediated Ca2+ influx in rat HCN cells in presence of NMDA receptor antagonist MK801 | 18552832 | ||
| HCN | Function assay | Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells | 18552832 | ||
| HCN | Function assay | Induction of L-type calcium channel/NMDA receptor-mediated Ca2+ influx in rat HCN cells in presence multiple inhibitors | 18552832 | ||
| HCN | Function assay | Induction of HDAC5 translocation in cytoplasm of rat HCN cells assessed as phosphorylated HDAC5 accumulation | 18552832 | ||
| HCN | Function assay | Induction of CAMK-mediated MREx3 activity in rat HCN cells by luciferase reporter gene assay in presence of 2.5 uM potassium channel inhibitor KN92 | 18552832 | ||
| HCN | Function assay | Induction of HDAC5 translocation in nucleus of rat HCN cells assessed as phosphorylated HDAC5 accumulation | 18552832 | ||
| HCN | Function assay | Induction of CAMK-mediated MREx3 activity in rat HCN cells by luciferase reporter gene assay in presence of 200 nM PKC inhibitor Go6976 | 18552832 | ||
| 点击查看更多细胞系数据 | |||||
生物活性
| 产品描述 | Isoxazole 9 (Isx-9)是一种成体神经发生的合成促进剂，通过引起成熟神经干细胞/前体细胞(NSPCs)的神经元分化而发挥作用。Isoxazole 9 (Isx-9) 可激活多条信号通路，包括 TGF-β 诱导的上皮-间质转化信号通路 (EMT) ，以及在心脏分化不同阶段的经典和非经典的 Wnt 信号通路。 |
|---|
| 体外研究(In Vitro) | ||||
| 体外研究活性 | 在NSPCs中，isoxazole 9增加细胞数量，并促进细胞分化。在OPCs中，isoxazole 9诱导细胞损伤。在生长的EPCs中，isoxazole 9减少微管形成，而不影响早期EPCs。[1] | |||
|---|---|---|---|---|
| 细胞实验 | 细胞系 | NSPCs,OPCs,早期EPCs，和副产物EPCs | ||
| 浓度 | ~50 μM | |||
| 孵育时间 | 48小时 | |||
| 方法 | 细胞用不同浓度的Isx-9 (0，6.25，12.5，25，或50 μM)处理，2天后，细胞数量以盲方式计数，以评估Isx-9对四种类型祖细胞/前体细胞的增殖/存活的作用。 | |||
| 体内研究(In Vivo) | ||
| 体内研究活性 | 在小鼠中，isoxazole 9 (20 mg/kg, i.p.)穿过血脑屏障，并通过Mef2-特异性机制，增加海马体SGZ中神经干细胞的增殖和神经发生。 Isoxazole 9 (20 mg/kg, i.p.)也会增加不成熟神经元的分化和树突复杂度，并改善记忆。在MWM中，isoxazole 9改善空间记忆。[2] | |
|---|---|---|
| 动物实验 | Animal Models | 转基因Nkx2.5-荧光素酶报告基因小鼠 |
| Dosages | 20 mg/kg | |
| Administration | i.p. | |
化学信息&溶解度
| 分子量 | 234.27 | 分子式 | C11H10N2O2S |
| CAS号 | 832115-62-5 | SDF | Download Isoxazole 9 (ISX-9) SDF |
| Smiles | C1CC1NC(=O)C2=NOC(=C2)C3=CC=CS3 | ||
| 储存条件(自收到货起) | |||
|
体外溶解度 |
DMSO : 47 mg/mL ( (200.62 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO) Ethanol : 12 mg/mL (51.22 mM) Water : Insoluble |
摩尔浓度计算器 |
|
体内溶解度 现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂 |
动物体内配方计算器 | ||||
实验计算
动物体内配方计算器（澄清溶液）
第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）
mg/kg
g
μL
只
第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）
% DMSO
%
% Tween 80
% ddH2O
%DMSO
%
计算结果：
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；
体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH2O，混匀澄清。
体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。
注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
技术支持
在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。
如果有其他问题，请给我们留言。
* 必填项
Tags: buy Isoxazole 9 (ISX-9) | Isoxazole 9 (ISX-9) supplier | purchase Isoxazole 9 (ISX-9) | Isoxazole 9 (ISX-9) cost | Isoxazole 9 (ISX-9) manufacturer | order Isoxazole 9 (ISX-9) | Isoxazole 9 (ISX-9) distributor
