Acetylcysteine (N-acetylcysteine)

别名: N-acetylcysteine 中文名称:乙酰半胱氨酸

Acetylcysteine (N-acetyl-l-cysteine, NAC,N-acetylcysteine)是ROS抑制剂,拮抗多种蛋白酶体抑制剂的活性。它还是肿瘤坏死因子TNF的抑制剂,主要用作祛痰剂,可通过维持或恢复肝脏中谷胱甘肽的浓度来处理扑热息痛(对乙酰氨基酚过量)。Acetylcysteine(N-acetyl-l-cysteine) 通过抑制IκB kinases抑制TNF诱导的NF-κB活化。Acetylcysteine(N-acetyl-l-cysteine) 通过线粒体依赖性途径诱导凋亡并抑制铁死亡和病毒复制。在溶液中不稳定,请现配现用!

Acetylcysteine (N-acetylcysteine) Chemical Structure

Acetylcysteine (N-acetylcysteine) Chemical Structure

CAS: 616-91-1

规格 价格 库存 购买数量
500mg 574.12 现货
1g 737.1 现货
5g 1203.93 现货
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常与Acetylcysteine (N-acetylcysteine)一起在实验中被使用的化合物

Ciprofloxacin


Acetylcysteine和Ciprofloxacin疗法被用作部分胆道梗阻的替代治疗选择。

Ozdil B, et al. J Clin Pharmacol. 2010 Dec;50(12):1414-9.

Tigecycline


Acetylcysteine和Tigecycline显着减少所有活的生物膜相关细菌。

Aslam S, et al. Antimicrob Agents Chemother. 2007 Apr; 51(4): 1556–1558.

Prostaglandin E2 (PGE2)


Acetylcysteine和Prostaglandin E2协同减少细胞凋亡,改善肝酶,预防死亡。

North TE, et al. Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17315-20.

Colistin


Acetylcysteine和Colistin在体外表现出协同作用Stenotrophomonas maltophilia

Ciacci N, et al. Antibiotics (Basel). 2019 Jul 25;8(3):101.

Acetylcysteine (N-acetylcysteine)相关产品

相关信号通路图

细胞实验数据示例

细胞系 实验类型 给药浓度 孵育时间 活性描述 文献信息
HUVEC cells Function assay 2μM,4μM,8μM 90min HUVECs that were exposed with serum of P. falciparum and treated with NAC 2 µM,4 µM,8 µM. Supernatant from culture and lysed cells culture were measured for H2O2, GSH and MDA levels. 21602763
HUVEC cells Function assay 10μM 2h HUVECs were pretreated with 10 μM NAC for 2 hours, and then treated with 0.5 μM PCB 118. presentce of NAC for 48 hours. 28592194
NHBE Cytotoxicity assay 1 mM 18 hrs Cytoprotective activity in NHBE cells assessed as inhibition of tBHP-induced GSH depletion at 1 mM preincubated for 18 hrs followed by tBHP addition for 4 hrs by thiostar dye based fluorescence assay 27031670
HUVEC Antioxidant assay 5 mmol/L 4 hrs Antioxidant activity against H2O2-induced lipid accumulation in HUVEC cells assessed as reduction in MDA level at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs 22841280
HUVEC Antioxidant assay 5 mmol/L 4 hrs Antioxidant activity in HUVEC cells assessed as reduction in H2O2-induced GSH activity at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs 22841280
HUVEC Cytotoxicity assay 5 mmol/L 4 hrs Inhibition of H2O2-induced cytotoxicity in HUVEC cells assessed as cell viability at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs by MTT assay 22841280
HUVEC Cytotoxicity assay 5 mmol/L 4 hrs Inhibition of H2O2-induced cytotoxicity in HUVEC cells assessed as LDH release at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs by LDH assay 22841280
SH-SY5Y Neuroprotective assay 5 mM 4 hrs Neuroprotective activity in H2O2-stimulated human SH-SY5Y cells assessed as upregulation of Bax expression at 5 mM incubated for 4 hrs prior to H2O2 challenge measured after 12 hrs by Western blotting analysis 23403085
SH-SY5Y Neuroprotective assay 5 mM 4 hrs Neuroprotective activity in H2O2-stimulated human SH-SY5Y cells assessed as downregulation of Bcl2 expression at 5 mM incubated for 4 hrs prior to H2O2 challenge measured after 12 hrs by Western blotting analysis 23403085
HT22 Neuroprotective assay 5 mM 24 hrs Neuroprotective activity against glutamate-induced cell death in mouse HT22 cells assessed as increase in cell viability at 5 mM after 24 hrs by MTT assay 29122481
HT22 Neuroprotective assay 50 uM 10 to 12 hrs Neuroprotective activity against glutamate-induced cell death in mouse HT22 cells assessed as reduction in apoptotic cells at 50 uM after 10 to 12 hrs by Annexin V-alexa 488/propidium iodide staining based flow cytometry 29122481
HL-7702 Hepatoprotective assay 10 uM 24 hrs Hepatoprotective activity against APAP-induced cell injury in human HL-7702 cells assessed as increase in survival rate at 10 uM pre-incubated for 24 hrs before APAP addition and measured 6 hrs post APAP challenge by MTT assay 28729056
PC12 Cytotoxicity assay 24 hrs Inhibition of 6-hydroxydopamine induced cytotoxicity in rat PC12 cells pretreated for 24 hrs assessed as elevation of intracellular glutathione level 17158454
BL21 (DE3) Function assay 30 mins Inhibition of hexahistidine-tagged IMP-7 (unknown origin) expressed in Escherichia coli BL21 (DE3) cells using fluorocillin as substrate incubated for 30 mins by TECAN fluorescent plate reader analysis, IC50 = 20.7 μM. 25815530
PC12 Cytotoxicity assay 24 hrs Inhibition of hydrogen peroxide induced cytotoxicity in rat PC12 cells pretreated for 24 hrs assessed as elevation of intracellular glutathione level 17158454
点击查看更多细胞系数据

生物活性

产品描述 Acetylcysteine (N-acetyl-l-cysteine, NAC,N-acetylcysteine)是ROS抑制剂,拮抗多种蛋白酶体抑制剂的活性。它还是肿瘤坏死因子TNF的抑制剂,主要用作祛痰剂,可通过维持或恢复肝脏中谷胱甘肽的浓度来处理扑热息痛(对乙酰氨基酚过量)。Acetylcysteine(N-acetyl-l-cysteine) 通过抑制IκB kinases抑制TNF诱导的NF-κB活化。Acetylcysteine(N-acetyl-l-cysteine) 通过线粒体依赖性途径诱导凋亡并抑制铁死亡和病毒复制。在溶液中不稳定,请现配现用!
靶点
NF-κB [8] Ferroptosis [9] ROS [6] TNF-α [7]
体外研究(In Vitro)
体外研究活性

N-acetylcysteine抑制c-Jun N末端激酶,P38 MAP激酶和氧化还原敏感激活蛋白1和核因子κB转录因子的活化,调节多种基因的表达。 N-acetylcysteine也能阻止细胞凋亡并通过激活细胞外信号调节激酶通路,是用于治疗某些退行性疾病有用的一个概念。N-acetylcysteine通过其还原活性直接调节几种蛋白质的活性。[1]

在没有其它营养支持的血清剥夺PC12细胞中,N-acetylcysteine可以防止细胞凋亡的DNA片段,并保持长期生存。 N-acetylcysteine还可以防止PC12细胞和交感神经元死亡。[2]

在大鼠和人的主动脉平滑肌细胞中,N-acetylcysteine剂量依赖性地减少存活率。[3]

在PC12细胞中,N-acetylcysteine激活Ras-外信号调节激酶(ERK)途径。N-acetylcysteine保护撤出营养支持诱发的神经元细胞免受死亡。 N-acetylcysteine增加存储在血管组织中一氧化氮(NO)的释放。 N-乙酰半胱氨酸预处理的PC12细胞干扰NGF-依赖的信号和神经突增生,并且有人提出,N-acetylcysteine干扰氧化还原敏感步骤。 [4]

体内研究(In Vivo)
体内研究活性

N-acetylcysteine提高了12月龄SAMP8小鼠在T型迷宫电击回避范式和杠杆记者食欲任务的认知能力,不引起电机活动,激励非特异性作用以避免震动,或体重。[5]

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT04520139 Not yet recruiting
Ovarian Cancer|Cognitive Impairment
University of California Irvine|Jarrow Formulas Inc
December 2024 Phase 1|Phase 2
NCT06112834 Not yet recruiting
Botulism
California Department of Public Health
June 2024 Phase 2
NCT06260566 Not yet recruiting
Biliary Atresia
Sanjiv Harpavat|Baylor College of Medicine
May 2024 Phase 1
NCT06377410 Not yet recruiting
Chronic Obstructive Pulmonary Disease
National University of Malaysia
May 1 2024 Not Applicable
NCT06223568 Not yet recruiting
Squamous Cell Carcinoma of the Head and Neck|Oropharynx|Human Papillomavirus Viruses|Drug Therapy|Cancer Vaccine
National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC)
May 15 2024 Phase 2

化学信息&溶解度

分子量 163.19 分子式

C5H9NO3S

CAS号 616-91-1 SDF Download Acetylcysteine (N-acetylcysteine) SDF
Smiles CC(=O)NC(CS)C(=O)O
储存条件(自收到货起) 3年-20°C 粉状 此产品性质不稳定,需现配现用!建议您购买分装规格,或者在收到货后进行分装。

体外溶解度
批次:

DMSO : 32 mg/mL ( (196.09 mM) ;DMSO吸湿会降低化合物溶解度,请使用新开封DMSO)

Water : 32 mg/mL (196.09 mM)

Ethanol : 32 mg/mL (196.09 mM)

摩尔浓度计算器

体内溶解度
批次:

现配现用,请按从左到右的顺序依次添加,澄清后再加入下一溶剂

动物体内配方计算器

实验计算

摩尔浓度计算器

质量 浓度 体积 分子量

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

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