human MDA435/LCC6MDR cells |
Function assay |
10 μM |
5 days |
Modulation of P-gp (unknown origin) transfected in human MDA435/LCC6MDR cells assessed as reversible of paclitaxel resistance measured as IC50 for paclitaxel at 10 uM after 5 days by CellTiter 96 Aqueous assay, IC50=0.1226 μM |
|
human Caco-2 cells |
Growth inhibition assay |
25 μM |
6 days |
Growth inhibition of human Caco-2 cells at 25 uM after 6 days |
|
mouse 3T3 cells |
Function assay |
1-20 μM |
12 h |
Antimigratory activity against Swiss albino mouse 3T3 cells assessed as increase of cell numbers at 1 to 20 uM after 12 hrs by by scratch-wound assay |
|
human Jurkat cells |
Function assay |
10-55 μM |
24 h |
Inhibition of TNFalpha-induced NF-kappaB activation in human Jurkat cells at 10 to 55 uM after 24 hrs by electrophoretic mobility shift assay |
|
human Raji cells |
Growth inhibition assay |
10 μM |
48 h |
Growth inhibition against human Raji cells assessed as cell viability at 10 uM after 48 hrs by trypan blue based microscopic analysis in presence of superoxide dismutase |
|
human PC3 cells |
Proliferation assay |
10-100 μM |
48 h |
Antiproliferative activity against human PC3 cells at 10 to 100 uM after 48 hrs by hemocytometric cell counting method |
|
human A431 cells |
Function assay |
100 μM |
12 h |
Reduction of clustering of GFP-GPI in lipid rafts of human A431 cells at 100 uM after 12 hrs by confocal microscopic analysis |
|
human SKBR3 cells |
Function assay |
200μM |
30 mins |
Downregulation of ErbB2 protein expression in human SKBR3 cells in serum free medium at 200 uM after 30 mins by immunofluorescence staining-based confocal microscopic analysis |
|
human 293T cells |
Function assay |
50 μM |
12 h |
Inhibition of DYRK1A in human 293T cells assessed as reduction of GLI1 transcription activity at 50 uM after 12 hrs by dual-luciferase reporter gene assay |
|
CHO cells |
Cytotoxic assay |
|
48 h |
Cytotoxicity against CHO cells expressing OATP1B3 haplotype 1 after 48 hrs by fluorescence based CellTiter-Glo assay, IC50=3.2 μM |
|
MDCK cells |
Function assay |
|
4 days |
Antiviral activity against influenza A virus (A/swine/OH/511445/2007(H1N1)) Oh7 infected in MDCK cells assessed as inhibition of viral replication after 4 days by quantitative RT-PCR, ED50=8.3 μM |
|
human HL60 cells |
Proliferation assay |
|
3 days |
Antiproliferative activity against human HL60 cells after 3 days, IC50=9.4 μM |
|
HSC-T6 cells |
Function assay |
|
48 h |
Antifibrotic activity against rat HSC-T6 cells assessed as inhibition of proliferation after 48 hrs by BrdU incorporation assay, IC50=9.9 μM |
|
mouse RAW264.7 cells |
Function assay |
|
5 days |
Inhibition of RANKL-induced osteoclastogenesis in mouse RAW264.7 cells assessed as decrease in TRAP-positive multi-nucleated cells after 5 days, IC50=29.8 μM |
|
human A431 cells |
Proliferation assay |
|
48 h |
Antiproliferative activity against human A431 cells overexpressing ErbB in serum-free medium assessed as cell viability after 48 hrs by WST-1 assay, EC50=38 μM |
|
human MDA-MB-231 cells |
Proliferation assay |
|
24 h |
Antiproliferative activity against human MDA-MB-231 cells after 24 hrs by MTT assay |
|
human HepG2 cells |
Function assay |
|
24 h |
Inhibition of oleic acid-induced triglyceride over-accumulation in human HepG2 cells incubated for 24 hrs relative to untreated control |
|
human HepG2 cells |
Function assay |
|
24 h |
Antioxidant activity in human HepG2 cells assessed as reduction of oleic acid-induced ROS generation incubated for 24 hrs by DHCF-DA based fluorimetric assay relative to untreated control |
|
human MDA-MB-231 cells |
Proliferation assay |
|
24 h |
Antiproliferative activity against human MDA-MB-231 cells after 24 hrs by MTT assay |
|
human K562 cells |
Function assay |
|
24 h |
Induction of 67 kDa laminin receptor expression in human K562 cells after 24 hrs by flow cytometry analysis |
|
human HL60 cells |
Function assay |
|
24 h |
Induction of 67 kDa laminin receptor expression in human HL60 cells after 24 hrs by flow cytometry analysis |
|
SH-SY5Y cells |
Function assay |
|
|
Neuroprotection against beta-amyloid peptide 1-42-induced toxicity in human SH-SY5Y cells assessed as lactate dehydrogenase release, EC50=0.03987 μM |
|
Sf9 cells |
Function assay |
|
|
Inhibition of His6-tagged human recombinant DNMT1 expressed in insect Sf9 cells assessed as reduction in DNA methyltransferase activity using 5'-biotinylated 45-bp unmethylated or hemimethylated oligonucleotide substrates and [3H]-AdoMet by liquid scintillation counting method, IC50=0.5 μM |
|
human U937 cells |
Function assay |
|
|
Inhibition of telomerase in human U937 cells, IC50=1 μM |
|
human HeLa cells |
Function assay |
|
|
Inhibition of telomerase in human HeLa cells using 5'-AAT CCG TCG AGC AGA GTT-3' as substrate incubated for 15 mins prior to extension reaction followed by compound washout by spin-telomeric repeat amplification protocol, IC50=1.08 μM |
|
mouse 3T3-L1 cells |
Function assay |
|
|
Inhibition of G6PD-mediated NADPH production in mouse 3T3-L1 cells, IC50=25 μM |
|
HSC-T6 cells |
Proliferation assay |
|
|
Antiproliferative activity against rat HSC-T6 cells assessed as reduction in cell viability, IC50=29.8 μM |
|