EGCG ((-)-Epigallocatechin Gallate)

中文名称:(-)-表没食子儿茶素没食子酸酯

EGCG ((-)-Epigallocatechin Gallate)是从绿茶中分离的一种有效的抗氧化剂Polyphenol flavonoid。抑制telomeraseDNA methyltransferase, 阻滞EGF受体HER-2受体的激活,抑制脂肪酸合成酶以及谷氨酸脱氢酶的活性。

EGCG ((-)-Epigallocatechin Gallate) Chemical Structure

EGCG ((-)-Epigallocatechin Gallate) Chemical Structure

CAS: 989-51-5

规格 价格 库存 购买数量
10mM (1mL in DMSO) 790 现货
50mg 647.01 现货
1g 3251.43 现货
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EGCG ((-)-Epigallocatechin Gallate)相关产品

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细胞实验数据示例

细胞系 实验类型 给药浓度 孵育时间 活性描述 文献信息
human MDA435/LCC6MDR cells Function assay 10 μM 5 days Modulation of P-gp (unknown origin) transfected in human MDA435/LCC6MDR cells assessed as reversible of paclitaxel resistance measured as IC50 for paclitaxel at 10 uM after 5 days by CellTiter 96 Aqueous assay, IC50=0.1226 μM 25985195
human Caco-2 cells Growth inhibition assay 25 μM 6 days Growth inhibition of human Caco-2 cells at 25 uM after 6 days 18324763
mouse 3T3 cells Function assay 1-20 μM 12 h Antimigratory activity against Swiss albino mouse 3T3 cells assessed as increase of cell numbers at 1 to 20 uM after 12 hrs by by scratch-wound assay 21080642
human Jurkat cells Function assay 10-55 μM 24 h Inhibition of TNFalpha-induced NF-kappaB activation in human Jurkat cells at 10 to 55 uM after 24 hrs by electrophoretic mobility shift assay 21080642
human Raji cells Growth inhibition assay 10 μM 48 h Growth inhibition against human Raji cells assessed as cell viability at 10 uM after 48 hrs by trypan blue based microscopic analysis in presence of superoxide dismutase 21434603
human PC3 cells Proliferation assay 10-100 μM 48 h Antiproliferative activity against human PC3 cells at 10 to 100 uM after 48 hrs by hemocytometric cell counting method 23867167
human A431 cells Function assay 100 μM 12 h Reduction of clustering of GFP-GPI in lipid rafts of human A431 cells at 100 uM after 12 hrs by confocal microscopic analysis 24456004
human SKBR3 cells Function assay 200μM 30 mins Downregulation of ErbB2 protein expression in human SKBR3 cells in serum free medium at 200 uM after 30 mins by immunofluorescence staining-based confocal microscopic analysis 24456004
human 293T cells Function assay 50 μM 12 h Inhibition of DYRK1A in human 293T cells assessed as reduction of GLI1 transcription activity at 50 uM after 12 hrs by dual-luciferase reporter gene assay 22154664
CHO cells Cytotoxic assay 48 h Cytotoxicity against CHO cells expressing OATP1B3 haplotype 1 after 48 hrs by fluorescence based CellTiter-Glo assay, IC50=3.2 μM 23327877
MDCK cells Function assay 4 days Antiviral activity against influenza A virus (A/swine/OH/511445/2007(H1N1)) Oh7 infected in MDCK cells assessed as inhibition of viral replication after 4 days by quantitative RT-PCR, ED50=8.3 μM 22115591
human HL60 cells Proliferation assay 3 days Antiproliferative activity against human HL60 cells after 3 days, IC50=9.4 μM 18693020
HSC-T6 cells  Function assay 48 h Antifibrotic activity against rat HSC-T6 cells assessed as inhibition of proliferation after 48 hrs by BrdU incorporation assay, IC50=9.9 μM 21504848
mouse RAW264.7 cells Function assay 5 days Inhibition of RANKL-induced osteoclastogenesis in mouse RAW264.7 cells assessed as decrease in TRAP-positive multi-nucleated cells after 5 days, IC50=29.8 μM 21456521
human A431 cells Proliferation assay 48 h Antiproliferative activity against human A431 cells overexpressing ErbB in serum-free medium assessed as cell viability after 48 hrs by WST-1 assay, EC50=38 μM 24456004
human MDA-MB-231 cells Proliferation assay 24 h Antiproliferative activity against human MDA-MB-231 cells after 24 hrs by MTT assay 22459208
human HepG2 cells Function assay 24 h Inhibition of oleic acid-induced triglyceride over-accumulation in human HepG2 cells incubated for 24 hrs relative to untreated control 21824690
human HepG2 cells Function assay 24 h Antioxidant activity in human HepG2 cells assessed as reduction of oleic acid-induced ROS generation incubated for 24 hrs by DHCF-DA based fluorimetric assay relative to untreated control 21824690
human MDA-MB-231 cells Proliferation assay 24 h Antiproliferative activity against human MDA-MB-231 cells after 24 hrs by MTT assay 22459208
human K562 cells Function assay 24 h Induction of 67 kDa laminin receptor expression in human K562 cells after 24 hrs by flow cytometry analysis 21434603
human HL60 cells Function assay 24 h Induction of 67 kDa laminin receptor expression in human HL60 cells after 24 hrs by flow cytometry analysis 21434603
SH-SY5Y cells Function assay Neuroprotection against beta-amyloid peptide 1-42-induced toxicity in human SH-SY5Y cells assessed as lactate dehydrogenase release, EC50=0.03987 μM 19138859
Sf9 cells Function assay Inhibition of His6-tagged human recombinant DNMT1 expressed in insect Sf9 cells assessed as reduction in DNA methyltransferase activity using 5'-biotinylated 45-bp unmethylated or hemimethylated oligonucleotide substrates and [3H]-AdoMet by liquid scintillation counting method, IC50=0.5 μM 25406944
human U937 cells Function assay Inhibition of telomerase in human U937 cells, IC50=1 μM 22413845
human HeLa cells Function assay Inhibition of telomerase in human HeLa cells using 5'-AAT CCG TCG AGC AGA GTT-3' as substrate incubated for 15 mins prior to extension reaction followed by compound washout by spin-telomeric repeat amplification protocol, IC50=1.08 μM 22413845
mouse 3T3-L1 cells Function assay Inhibition of G6PD-mediated NADPH production in mouse 3T3-L1 cells, IC50=25 μM 18313308
HSC-T6 cells Proliferation assay Antiproliferative activity against rat HSC-T6 cells assessed as reduction in cell viability, IC50=29.8 μM 25322455
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生物活性

产品描述 EGCG ((-)-Epigallocatechin Gallate)是从绿茶中分离的一种有效的抗氧化剂Polyphenol flavonoid。抑制telomeraseDNA methyltransferase, 阻滞EGF受体HER-2受体的激活,抑制脂肪酸合成酶以及谷氨酸脱氢酶的活性。
靶点
telomerase [4] DNMT [4] HER2 [5] EGFR [5] FASN [6]
体外研究(In Vitro)
体外研究活性

(-)-Epigallocatechin gallate在健康细胞中具有强效抗氧化作用,并降低氧化损伤,作为抗血管生成和抗肿瘤剂,也是肿瘤细胞对化疗应答的调节剂。(-)-Epigallocatechin gallate具有多种抗肿瘤作用,例如抗增殖,抗血管新生,阻断各种癌细胞转化,细胞周期阻滞和抑制肿瘤转移。(-)-Epigallocatechin gallate通过调节多个肿瘤相关的细胞信号通路(调节关键信号蛋白的表达水平,例如核因子-κB, MAPKs和激活蛋白-1,EGFR,IGF,COX-2)影响肿瘤基因的甲基化和配体与膜受体结合发挥抗肿瘤效果。[1] (-)-Epigallocatechin gallate具有免疫调节作用。多种类型的免疫细胞在先天的和适应性免疫系统中都会不同程度受到(-)-Epigallocatechin gallate的影响。在这些作用中,对T细胞的显著作用被反复证明,包括T细胞激活,增殖,分化和细胞因子产生。基于患有自身免疫疾病的动物模型的研究报道表明,绿茶/EGCG处理会使动物的病情好转。[2] (-)-Epigallocatechin gallate具有抗感染活性。(-)-Epigallocatechin gallate的以不同激活模式产生的抗病毒活性在很多种属的病毒中被证实,例如逆转录病毒,正粘病毒科和黄病毒科,还包括重要的人类病原细菌,如人类免疫缺陷病原体,甲型流感病毒和丙型肝炎病毒。[3]

实验图片 检测方法 检测指标 实验图片 PMID
Western blot β-catenin / p-AKT / Cyclin D1 p-MAPK / MAPK Notch1 / Notch2 28693189
Growth inhibiton assay Cell proliferation Cell viability 23525843
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05758571 Recruiting
Interstitial Pneumonia|Neoplasms Malignant
Shandong Cancer Hospital and Institute
January 5 2023 Phase 1|Phase 2

化学信息&溶解度

分子量 458.37 分子式

C22H18O11

CAS号 989-51-5 SDF Download EGCG ((-)-Epigallocatechin Gallate) SDF
储存条件(自收到货起)

体外溶解度
批次:

DMSO : 92 mg/mL ( (200.71 mM) ;DMSO吸湿会降低化合物溶解度,请使用新开封DMSO)

Ethanol : 92 mg/mL (200.71 mM)

Water : 20 mg/mL (43.63 mM)

摩尔浓度计算器

体内溶解度
批次:

现配现用,请按从左到右的顺序依次添加,澄清后再加入下一溶剂

动物体内配方计算器

实验计算

摩尔浓度计算器

质量 浓度 体积 分子量

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

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