Rotenone
别名: Barbasco, Dactinol, Paraderil, Rotenon, Rotocide 中文名称：鱼藤酮
Rotenone是一种植物杀虫剂，是线粒体电子传递mitochondrial electron transport的抑制剂。Rotenone 可抑制 NADH/DB oxidoreductase 和 NADH oxidase，对应的IC50值分别为28.8 nM和5.1 nM。Rotenone 可通过增强线粒体活性氧的产生来诱导凋亡。
Rotenone Chemical Structure
CAS: 83-79-4
产品质控
批次：
纯度：
99.97%
99.97
Rotenone相关产品
| 相关靶点 | NOX1 NOX4 NOX2 | 点击展开 |
|---|---|---|
| 相关产品 | Setanaxib (GKT137831) DPI (Diphenyleneiodonium chloride) VAS2870 GSK2795039 GLX351322 2-Acetylphenothiazine (ML171) | 点击展开 |
| 相关化合物库 | 激酶抑制剂库 FDA药物库 天然产物库 已知活性药物库-I 高选择性抑制剂库 | 点击展开 |
细胞实验数据示例
| 细胞系 | 实验类型 | 给药浓度 | 孵育时间 | 活性描述 | 文献信息 |
|---|---|---|---|---|---|
| MG63 | Function assay | 1 uM | 1 hr | Increase in lactate production in MG63 cells at 1 uM after 1 hr by ELISA | 17088489 |
| N548 | Activation of AKT in | 10 uM | 6 hrs | Activation of AKT in DNA-deficient rat striatal neuronal N548 mutant cells at 10 uM after 6 hrs by Western blotting | 17726098 |
| N548 | Cell death rescue assay | 10 uM | 7 days | Cell death rescue in rat striatal neuronal N548 mutant cells analysed every day for 7 days at 10 uM by trypan blue dye-exclusion assay | 17726098 |
| T47D | Function assay | 0.01 to 0.1 uM | 72 hrs | Reduction in ATP level in human T47D cells at 0.01 to 0.1 uM after 72 hrs by HPLC-UV analysis | 21875114 |
| T47D | Function assay | 0.1 to 1 uM | 30 mins | Inhibition of mTOR in human T47D cells assessed as eIF2alpha hyperphosphorylation on Ser 51 at 0.1 to 1 uM after 30 mins by Western blotting | 21875114 |
| T47D | Function assay | 0.1 to 1 uM | 30 mins | Inhibition of mTOR in human T47D cells assessed as eEF2 hyperphosphorylation on Ser 56 at 0.1 to 1 uM after 30 mins by Western blotting | 21875114 |
| MDA-MB-231 | Function assay | 0.1 to 1 uM | 30 mins | Inhibition of mTOR in human MDA-MB-231 cells assessed as eIF2alpha hyperphosphorylation on Ser 51 at 0.1 to 1 uM after 30 mins by Western blotting | 21875114 |
| MDA-MB-231 | Function assay | 0.1 to 1 uM | 30 mins | Inhibition of mTOR in human MDA-MB-231 cells assessed as eEF2 hyperphosphorylation on Ser 56 at 0.1 to 1 uM after 30 mins by Western blotting | 21875114 |
| MDA-MB-231 | Function assay | 0.01 to 0.1 uM | 48 hrs | Reduction in ATP level in human MDA-MB-231 cells at 0.01 to 0.1 uM after 48 hrs by HPLC-UV analysis | 21875114 |
| N548 | Activation of ERK in | 10 uM | Activation of ERK in rat striatal neuronal N548 mutant cells at 10 uM by Western blotting | 17726098 | |
| N548 | Activation of AKT in | 10 uM | Activation of AKT in rat striatal neuronal N548 mutant cells at 10 uM by Western blotting | 17726098 | |
| N548 | Activation of ERK in | 50 uM | Activation of ERK in rat striatal neuronal N548 mutant cells at 50 uM by Western blotting | 17726098 | |
| N548 | Activation of AKT in | 50 uM | Activation of AKT in rat striatal neuronal N548 mutant cells at 50 uM by Western blotting | 17726098 | |
| N548 | Cell death rescue assay | 10 uM | Cell death rescue in DNA-deficient rat striatal neuronal N548 mutant cells at 10 uM | 17726098 | |
| T47D | Function assay | 0.001 to 0.1 uM | Inhibition of cellular respiration in human T47D cells assessed as rate of oxygen consumption at 0.001 to 0.1 uM | 21875114 | |
| TA3/Ha | Function assay | 12 uM | Induction of NAD(P)H oxidation in mouse TA3/Ha cells assessed as reduction of NAD(P)H/NAD(P)+ ratio at 12 uM by spectrofluorometer analysis | 24568614 | |
| U937 | Antiproliferative assay | 72 hrs | Antiproliferative activity against human U937 cells after 72 hrs by WST-8 assay, IC50=0.07μM | 17158054 | |
| U937 | Antiproliferative assay | 48 hrs | Antiproliferative activity against human U937 cells after 48 hrs by WST-8 assay, IC50=0.2μM | 17158054 | |
| U937 | Antiproliferative assay | 24 hrs | Antiproliferative activity against human U937 cells after 24 hrs by WST-8 assay, IC50=13μM | 17158054 | |
| HepG2 | Antitumor assay | 24 hrs | Antitumor activity against human HepG2 cells after 24 hrs by MTT assay, ED50=15μM | 17482824 | |
| A549 | Antitumor assay | 24 hrs | Antitumor activity against human A549 cells after 24 hrs by MTT assay, ED50=26μM | 17482824 | |
| U373 MG | Antiproliferative assay | 72 hrs | Antiproliferative activity against human U373 MG cells after 72 hrs | 18513974 | |
| T47D | Function assay | 48 hrs | Reduction in ATP level in human T47D cells after 48 hrs by HPLC-UV analysis | 21875114 | |
| 697 | Cytotoxicity assay | 72 hrs | Cytotoxicity against human 697 cells after 72 hrs by MTS assay, IC50=0.3μM | 23895019 | |
| 697 | Cytotoxicity assay | 48 hrs | Cytotoxicity against human 697 cells after 48 hrs by MTS assay, IC50=0.3μM | 23895019 | |
| HT-29 | Cytotoxicity assay | 72 hrs | Cytotoxicity against human HT-29 cells after 72 hrs by SRB assay, IC50=0.3μM | 23895019 | |
| Raji | Cytotoxicity assay | 72 hrs | Cytotoxicity against human Raji cells after 72 hrs by MTS assay, IC50=1.1μM | 23895019 | |
| Raji | Cytotoxicity assay | 48 hrs | Cytotoxicity against human Raji cells after 48 hrs by MTS assay, IC50=4.2μM | 23895019 | |
| HepG2 | Cytotoxicity assay | 24 hrs | Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay, IC50=1.52μM | 30613327 | |
| HEK293 | Function assay | Effect on cofilin1 expressed in HEK293 cells assessed as effect on cofilin1; Limk2 interaction complexes in presence of camptothecin by EYFP and/or YFP Venus fragment based reporter gene assay | 16680159 | ||
| HEK293 | Function assay | Inhibition of of c-Jun expressed in HEK293 cells assessed as induction of protein interaction in presence of camptothecin with Pin1 by EYFP based reporter gene assay | 16680159 | ||
| HEK293 | Function assay | Effect on p53 expressed in HEK293 cells assessed as effect on p53-p53 interaction complexes in presence of camptothecin by EYFP and/or YFP Venus fragment based reporter gene assay | 16680159 | ||
| HEK293 | Function assay | Effect on Cdc2 expressed in HEK293 cells assessed as effect on Cdc2:Cdc25A interaction complexes in presence of camptothecin by EYFP and/or YFP Venus fragment based reporter gene assay | 16680159 | ||
| HEK293 | Function assay | Effect on Cdc2 expressed in HEK293 cells assessed as effect on Cdc2:Cdc25C interaction complexes in presence of camptothecin by EYFP and/or YFP Venus fragment based reporter gene assay | 16680159 | ||
| HT1080 | Function assay | Inhibition of proMMP2 production in human HT1080 cells by gelatin zymography analysis | 17158054 | ||
| HT1080 | Function assay | Inhibition of proMMP9 production in human HT1080 cells by gelatin zymography analysis | 17158054 | ||
| neural precursor cells | Function assay | Inhibition of neurosphere proliferation of mouse neural precursor cells by MTT assay | 17417631 | ||
| N548 | Cell death rescue assay | Cell death rescue in rat striatal neuron N548 mutant cells, EC50=0.5μM | 17726098 | ||
| ASH neuronal cells | Cell death rescue assay | Cell death rescue of ASH neuronal cells in Caenorhabditis elegans Huntington's disease model, EC50=2μM | 17726098 | ||
| neuronal cells | Cell death rescue assay | Cell death rescue of neuronal cells in drosophila Huntington's disease model, EC50=10μM | 17726098 | ||
| N548 | Toxicity assay | Toxicity in rat striatal neuronal N548 mutant cells, TC50=40μM | 17726098 | ||
| N548 | Cell death rescue assay | Suppression of cell death in rat striatal neuronal N548 mutant cells assessed as suppression of caspase 7 activation by Western blotting | 17726098 | ||
| N548 | Cell death rescue assay | Cell death rescue in rat striatal neuronal N548 mutant cells by calcein acetoxymethyl ester assay | 17726098 | ||
| N548 | Cell death rescue assay | Suppression of cell death in rat striatal neuronal N548 mutant cells assessed as suppression of caspase 3 activation by Western blotting | 17726098 | ||
| Hepa-1c1c7 | Cytotoxicity assay | Cytotoxicity against mouse Hepa-1c1c7 cells, IC50=0.7μM | 23895019 | ||
| Hepa1c1c7 | Function assay | Induction of quinone reductase in mouse Hepa1c1c7 cells, CD=0.8μM | 23895019 | ||
| L5178Y | Cytotoxicity assay | Cytotoxicity against mouse L5178Y cells by MTT assay, IC50=0.3μM | 28075580 | ||
| TC32 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for TC32 cells | 29435139 | ||
| DAOY | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for DAOY cells | 29435139 | ||
| SJ-GBM2 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells | 29435139 | ||
| A673 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells | 29435139 | ||
| SK-N-MC | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells | 29435139 | ||
| BT-37 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells | 29435139 | ||
| NB-EBc1 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells | 29435139 | ||
| Saos-2 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells | 29435139 | ||
| LAN-5 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for LAN-5 cells | 29435139 | ||
| BT-12 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells | 29435139 | ||
| OHS-50 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells | 29435139 | ||
| Rh41 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells | 29435139 | ||
| A673 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for A673 cells) | 29435139 | ||
| SK-N-MC | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-MC cells | 29435139 | ||
| BT-12 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for BT-12 cells | 29435139 | ||
| LAN-5 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for LAN-5 cells | 29435139 | ||
| NB-EBc1 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for NB-EBc1 cells | 29435139 | ||
| BT-37 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for BT-37 cells | 29435139 | ||
| TC32 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for TC32 cells | 29435139 | ||
| MG 63 (6-TG R) | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for MG 63 (6-TG R) cells | 29435139 | ||
| fibroblast cells | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for control Hh wild type fibroblast cells | 29435139 | ||
| OHS-50 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for OHS-50 cells | 29435139 | ||
| DLD1 | Function assay | Inhibition of mitochondrial oxidative phosphorylation in human DLD1 cells assessed as reduction in oxygen consumption rate by seahorse XFe96 analyser based assay | 31774672 | ||
| 点击查看更多细胞系数据 | |||||
生物活性
| 产品描述 | Rotenone是一种植物杀虫剂，是线粒体电子传递mitochondrial electron transport的抑制剂。Rotenone 可抑制 NADH/DB oxidoreductase 和 NADH oxidase，对应的IC50值分别为28.8 nM和5.1 nM。Rotenone 可通过增强线粒体活性氧的产生来诱导凋亡。 |
|---|
化学信息&溶解度
| 分子量 | 394.42 | 分子式 | C23H22O6 |
| CAS号 | 83-79-4 | SDF | Download Rotenone SDF |
| 储存条件(自收到货起) | |||
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体外溶解度 |
DMSO : 40 mg/mL ( (101.41 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO) Water : Insoluble Ethanol : Insoluble |
摩尔浓度计算器 |
|
体内溶解度 现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂 |
动物体内配方计算器 | ||||
实验计算
动物体内配方计算器（澄清溶液）
第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）
mg/kg
g
μL
只
第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）
% DMSO
%
% Tween 80
% ddH2O
%DMSO
%
计算结果：
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；
体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH2O，混匀澄清。
体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。
注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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