Berberine chloride

别名: NSC 646666, Natural Yellow 18 chloride 中文名称：盐酸小檗碱，黄连素

目录号：S2271 Purity: 99.75%

Berberine chloride是异喹啉类生物碱的季铵盐。Berberine chloride 可激活 caspase 3 和 caspase 8，分裂 poly ADP-ribose polymerase (PARP) 和释放 cytochrome c。Berberine chloride 可降低 c-IAP1，Bcl-2 和 Bcl-XL 的表达。Berberine chloride 可通过 JNK 和 p38 MAPK 的持续磷酸化以及产生 ROS 来诱导凋亡。Berberine chloride 是一个 topoisomerase I 和 II 的双效抑制剂。Berberine chloride 也是一个潜在的自噬调节剂。

Berberine chloride Chemical Structure

CAS: 633-65-8

规格	价格	库存
10mM (1mL in DMSO)	744.27	现货
10mg	571.33	现货
50mg	794.43	现货
200mg	1040.13	现货
1g	5217.03	现货
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产品质控

批次：纯度： 99.75%

99.75

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细胞实验数据示例

细胞系	实验类型	给药浓度	孵育时间	活性描述	文献信息
MRC5 cells	Proliferation assay	10 μM	54 hrs	Inhibition of HCMV proliferation in MRC5 cells after 54 hrs post-infection at 10 uM by plaque assay	17239594
MRC5 cells	Proliferation assay	10 μM	24 h	Inhibition of HCMV proliferation in MRC5 cells after 24 hrs post-infection at 10 uM by plaque assay	17239594
HepG2 cells	Function assay	10 ug/mL	12 h	Induction of LDLR protein expression in human HepG2 cells at 10 ug/mL after 12 hrs by flow cytometry	19090767
A10 cells	Function assay	30 μM	24 hrs	Downregulation of Scd2 mRNA expression in rat A10 cells at 30 uM after 24 hrs by quantitative RT-PCR analysis	21401114
A10 cells	Function assay	30 μM	24 hrs	Down regulation of Prim2 mRNA expression in rat A10 cells at 30 uM after 24 hrs by quantitative RT-PCR analysis	21401114
A10 cells	Function assay	30 μM	24 hrs	Downregulation of Impk mRNA expression in rat A10 cells at 30 uM after 24 hrs by quantitative RT-PCR analysis	21401114
HepG2 cells	Function assay	10 μM	4 h	Increase in AMPKalpha phosphorylation in human HepG2 cells at 10 uM after 4 hrs by Western blot analysis relative to untreated control	23058107
HepG2 cells	Function assay	10 μM	4 h	Increase in total AMPKalpha level in human HepG2 cells at 10 uM after 4 hrs by Western blot analysis relative to untreated control	23058107
HepG2 cells	Function assay	20 μM	24 hrs	Induction of apoptosis in human HepG2 cells assessed as morphological changes at 20 uM after 24 hrs using Hoechst 33258 staining by fluorescence microscopic analysis	23182088
HepG2-A16-CD81 cells	Function assay	10 μM		NOVARTIS: Antimalarial liver stage activity measured as a greater than 50% reduction in Plasmodium yoelii schizont area in HepG2-A16-CD81 cells at 10uM compound concentration, determined by immuno-fluorescence.	22096101
HepG2-A16-CD81 cells	Function assay	10 μM		NOVARTIS: Antimalarial liver stage activity measured as reduction in Plasmodium yoelii schizont area in HepG2-A16-CD81 cells by immuno-fluorescence, and median schizont size at 10uM compound concentration, IC50=0.548 μM	22096101
Bel7402 cells	Function assay		12 h	Induction of LDLR protein in human Bel7402 cells after 12 hrs by RT-PCR assay relative to control	19090767
KB cells	Cytotoxicity assay		72 h	Cytotoxicity against human KB cells after 72 hrs, IC50=7.32 μM	11141105
HL60 cells	Apoptosis assay		48 hrs	Induction of apoptosis in human HL60 cells after 48 hrs using annexin V-propidium iodide staining by FACS analysis	20227144
CEM cells	Cytotoxicity assay		48 hrs	Cytotoxicity against human CEM cells expressing green fluorescent protein after 48 hrs by MTT assay, CC50=2.09 μM	21295891
human CEM cells	Function assay		7 days	Antiviral activity against 0.05 MOI Human immunodeficiency virus 1 NL4.3 infected in human CEM cells expressing green fluorescent protein assessed as p24 antigen production measured 7 days post infection by ELISA, EC50=0.13 μM	21295891
SKN cells	Growth inhibition assay		72 h	Growth inhibition against human SKN cells after 72 hrs by MTT assay, GI50=15.88 μM	21401114
RKN cells	Growth inhibition assay		48 hrs	Growth inhibition against human RKN cells after 48 hrs by MTT assay, GI50=49.6 μM	21401114
G402 cells	Growth inhibition assay		48 hrs	Growth inhibition against human G402 cells after 48 hrs by MTT assay, GI50=11.87 μM	21401114
HT-29 cells	Cytotoxicity assay		48 hrs	Cytotoxicity against human HT-29 cells after 48 hrs by MTT assay, IC50=8.45 μM	23182088
HepG2 cells	Cytotoxicity assay		24 hrs	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay, IC50=11.22 μM	23182088
HepG2 cells	Cytotoxicity assay		48 hrs	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay, IC50=8.32 μM	23182088
MRC5 cells	Function assay			Antiviral activity against HCMV in MRC5 cells by plaque reduction assay, IC50=0.68 μM	17239594
A549 cells	Cytotoxicity assay			Cytotoxicity against human A549 cells by SRB assay, IC50=6.27 μM	20594848
SKOV3 cells	Cytotoxicity assay			Cytotoxicity against human SKOV3 cells by SRB assay, IC50=16.44 μM	20594848
SK-MEL-2 cells	Cytotoxicity assay			Cytotoxicity against human SK-MEL-2 cells by SRB assay, IC50=13.76 μM	20594848
HCT15 cells	Cytotoxicity assay			Cytotoxicity against human HCT15 cells by SRB assay, IC50=16.59 μM	20594848
点击查看更多细胞系数据

生物活性

产品描述

靶点

Caspase-3 ^[1]

Caspase-8 ^[1]

PARP ^[1]

cytochrome c ^[1]

cIAP1 ^[1]

点击更多

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
临床试验信息 (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT06273241	Not yet recruiting	Pharmacokinetic Study in Healthy Volunteers	University Medicine Greifswald	March 4 2024	Not Applicable
NCT05845931	Recruiting	Pharmacokinetic Study in Healthy Volunteers	University Medicine Greifswald	May 5 2023	Not Applicable
NCT05480670	Completed	Polycystic Ovary Syndrome	Ayub Teaching Hospital	November 1 2022	Not Applicable
NCT05463003	Completed	Pharmacokinetic Study in Healthy Volunteers	University Medicine Greifswald	July 19 2022	Not Applicable

参考文献
[1] Wen-Hsiu Hsu, et al. Arch Toxicol. 2007 Oct;81(10):719-28. [2] Marta Cernáková, et al. BMC Complement Altern Med. 2002 Feb 19;2:2. [3] Wang K, et al. Front Pharmacol. 2021;12:632201.

参考文献

化学信息&溶解度

分子量	371.81	分子式	C₂₀H₁₈NO₄.Cl
CAS号	633-65-8	SDF	Download Berberine chloride SDF
储存条件(自收到货起)	3年-20°C粉状	储备液保存和期限建议分装储备液，避免反复冻融！	1年-80°C溶于溶剂
储存条件(自收到货起)	3年-20°C粉状	储备液保存和期限建议分装储备液，避免反复冻融！	1个月-20°C溶于溶剂

体外溶解度
批次：

DMSO : 25 mg/mL ( (67.23 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Water : Insoluble

Ethanol : Insoluble

DMSO : 38 mg/mL ( (102.2 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Water : Insoluble

Ethanol : Insoluble

摩尔浓度计算器

体内溶解度
批次：

现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂

动物体内配方计算器

实验计算

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×

稀释计算器分子量计算器

动物体内配方计算器（澄清溶液）

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 μL 动物数量只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

计算结果：

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH₂O，混匀澄清。

体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。

注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

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操作手册

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* 必填项

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