BAY 11-7082

别名: BAY 11-7821

目录号：S2913 Purity: 99.98%

BAY 11-7082 (BAY 11-7821)是一种 NF-κB 抑制剂，抑制TNFα诱导的 IκBα 磷酸化，在肿瘤细胞中IC50为10 μM。BAY 11-7082 可抑制 ubiquitin-specific protease USP7 和 USP21，对应的IC50值分别为0.19 μM和0.96 μM。BAY 11-7082 在胃癌细胞中可诱导凋亡和S期阻滞。

BAY 11-7082 Chemical Structure

CAS: 19542-67-7

规格	价格	库存
10mg	738.58	现货
50mg	2223.41	现货
1g	16298.1	现货
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产品质控

批次：纯度： 99.98%

99.98

BAY 11-7082相关产品

相关靶点	IκB IKK	点击展开
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相关化合物库	FDA药物库免疫/炎症分子化合物库小分子免疫肿瘤化合物库 NF-κB信号通路库 FDA抗癌药物库	点击展开

细胞实验数据示例

细胞系	实验类型	给药浓度	孵育时间	活性描述	文献信息(PMID)
SP6.5	Growth Inhibition Assay	2.5-20 μM	24 h	IC50=5 μM, exhibits strong anti-proliferative effects in a dose-dependent manner	23443086
OM431	Function Assay	5 μM	2 h	decreases translocation of p65 in the nucleus	23443086
OCM1	Function Assay	5 μM	2 h	decreases translocation of p65 in the nucleus	23443086
VUP	Function Assay	5 μM	2 h	decreases translocation of p65 in the nucleus	23443086
SP6.5	Function Assay	5 μM	2 h	decreases translocation of p65 in the nucleus	23443086
BAFs	Function Assay	0.5/1 μM	24 h	inhibits TNFα/DEX induced CYP19A1 transcripts	23485457
KMS-12-BM	Apoptosis Assay	30 µM	3 h	induces MM cell death involves necrosis	23527154
MM.1S	Apoptosis Assay	30 µM	3 h	induces MM cell death involves necrosis	23527154
HT29	Function Assay	10/30/100 μM	1 h	inhibits TNF-induced phosphorylation and degradation of IκBα	23527154
HT29	Function Assay	10/30/100 μM	1 h	inhibites both TWEAK-induced p100 processing	23527154
HUVEC	Function Assay	10 μM	0.5 h	counteractes the loss of Tie2 mRNA	23563632
RAW 264	Function Assay	0.2-5 µM	30/60/90 min	inhibits the phosphatase activity of PTP1B	23578302
AM	Growth Inhibition Assay	5 µM	4/8 d	reduces the viability of intracellular MTB	23634218
MDM	Growth Inhibition Assay	5 µM	4/8 d	reduces the viability of intracellular MTB	23634218
THP-1	Growth Inhibition Assay	5 µM	4/8 d	reduces the viability of intracellular MTB	23634218
THP-1	Function Assay	5 µM	1 h	inhibits MTB-induced NFκB activation	23634218
HepG2	Function Assay	0.3/1/3 μM	48 h	reduces IL6-induced PON1 expression	23791833
HBE	Function Assay	10μM	3h	abolishes the increases of IL-6 expression induced by CSE	23824089
HGC-27	Apoptosis Assay	7.5/15/30 μM	6 h	induces apoptosis in a time- and dose-dependent manner	23846545
MGC80-3	Function Assay	7.5/15/30 μM	6 h	induces the dephosphorylation and up-regulation of IκBα	23846545
HGC-27	Function Assay	7.5/15/30 μM	6 h	induces the dephosphorylation and up-regulation of IκBα	23846545
MGC80-3	Growth Inhibition Assay	10/20/30 μM	6/24 h	suppresses cell viability significantly	23846545
AGS	Growth Inhibition Assay	10/20/30 μM	6/24 h	suppresses cell viability significantly	23846545
NCI-N87	Growth Inhibition Assay	10/20/30 μM	6/24 h	suppresses cell viability significantly	23846545
A549	Apoptosis Assay	1 μM	48 h	reduces the cell death induced by combined stimulation	23900080
A549	Function Assay	1 μM	48 h	enhances the up-regulation of IκB and subsequent decrease in Bax expression induced by combined stimulation	23900080
THP-1	Function Assay	10 μM	1 h	abolishes the effect of rHSP27 on SR-A mRNA	23939398
PC-3	Function Assay	2.5/5/10 μM	0.5 h	blocks IGF-II-induced STS mRNA expression	24055520
iNKT	Function Assay	10/100 μM	0.5 h	inhibits the induction of A2AR mRNA and other factor	24124453
IVD	Function Assay	10 μM	3 d	abrogates TNF-α–induced up-regulation of ADAMTS-4 and ADAMTS-5	24176808
IVD	Function Assay	10 μM	3 d	reverses TNF-α–mediated suppression of the disc matrix macromolecules aggrecan and collagen II	24176808
A549RT-eto	Function Assay	10 μM	24 h	decreases the expression levels of NF-κB and P-gp	24220725
A549RT-eto	Apoptosis Assay	10 μM	24 h	accelerates F14 extract-mediated apoptosis combined treatment with F14	24220725
WPs	Function Assay	25 μM	5 min	suppresses ATP and vWF secretion	24331207
DSCs	Function Assay	2.5 μM	0.5 h	reverses the enhancement of CCL2/CCR2 expression of DSCs induced by IL-33	24344240
OVCXCR2	Function Assay	2 µM	48 h	blocks the CXCL1-induced cell invasion	24376747
SKCXCR2	Function Assay	2 µM	48 h	blocks the CXCL1-induced cell invasion	24376747
SKCXCR2	Growth Inhibition Assay	2 µM	48 h	decreases cell proliferation significantly	24376747
THP-1	Function Assay	0.1/1 μM	0.5 h	abrogates TNF-α secretion as well as the increased secretion of IL-6 and IL-1β	24378536
A549RT-eto	Apoptosis Assay	10 μM	24 h	accelerates FERO-mediated apoptosis	24535083
HUVEC	Function Assay	20 µM	0.5 h	prevents the induction of EAM expression	24551209
A549	Function Assay	10 μM	1 h	inhibits the increase of phospho-IκBα in PA103-infected cultures	24612488
HeLa	Function Assay	5 μM	24 h	reduces the activity of TNF-α promoter	24657783
HUVECs	Function Assay	3-30 μM	1 h	reduces the expression of miR-146a in a dose-dependent manner	24863965
macrophages	Function Assay	5 µM	3 h	partially blocks YPFS-induced expression of iNOS and COX-2	24967898
RAW 264.7	Function Assay	5 μM	1 h	inhibits TNF-α and IL-12 p40 production	25019567
A549	Function Assay	10 µM	12 h	suppresses Dvl-3 induced activation of p65	25156800
HMECs	Function Assay	5 μM	2 h	abolishes TNF-α-induced VCAM-1 expression	25193116
HCT116	Function Assay	5 μM	2 h	attenuates silymarin-induced downregulation of cyclin D1	25479723
ARPE-19	Function Assay	1 μM	0.5 h	suppresses TG-induced IL-8 promoter activation	25593029
SiHa	Function Assay	10 μM	1.5 h	abolishes BPA induced up regulation of FN and MMP-9	25797437
HeLa	Function Assay	10 μM	1.5 h	abolishes BPA induced up regulation of FN and MMP-9	25797437
VUP	Growth Inhibition Assay	2.5-20 μM	24 h	IC50=5 μM, exhibits strong anti-proliferative effects in a dose-dependent manner	23443086
OCM1	Growth Inhibition Assay	2.5-20 μM	24 h	IC50=5 μM, exhibits strong anti-proliferative effects in a dose-dependent manner	23443086
OM431	Growth Inhibition Assay	2.5-20 μM	24 h	IC50=5 μM, exhibits strong anti-proliferative effects in a dose-dependent manner	23443086
SP6.5	Apoptosis Assay	5 μM	24 h	induces apoptosis	23443086
VUP	Apoptosis Assay	5 μM	24 h	induces apoptosis	23443086
OCM1	Apoptosis Assay	5 μM	24 h	induces apoptosis	23443086
OM431	Apoptosis Assay	5 μM	24 h	induces apoptosis	23443086
SP6.5	Function Assay	5 μM	12 h	reduces the migration	23443086
VUP	Function Assay	5 μM	12 h	reduces the migration	23443086
OCM1	Function Assay	5 μM	12 h	reduces the migration	23443086
OM431	Function Assay	5 μM	12 h	reduces the migration	23443086
HBL-1	Growth Inhibition Assay	3 μM	24/48/72 h	slows cell growth modestly	23441730
RAW 264.7	Function Assay	2-15 μM	1 h	suppresses the activation of IKK family members	23441730
IL-1R	Function Assay	2-15 μM	1 h	suppresses the activation of IKK family members	23441730
RAW 264.7	Function Assay	15 μM	1 h	suppresses the activation of and JNK	23441730
IL-1R	Function Assay	15 μM	1 h	suppresses the activation of and JNK	23441730
U2OS	Function Assay	15 μM	1 h	prevents the LPS- or IL-1-stimulated formation of K63-pUb chains	23441730
MT‐1	Function Assay	8 µm	3 h	decreases the levels of p‐STAT3 and p‐4EBP1	23278479
MT‐2	Function Assay	8 µm	3 h	decreases the levels of p‐STAT3 and p‐4EBP1	23278479
MT‐1	Function Assay	8 µm	3 h	decreases the levels of the p65 subunit of NF‐κB	23278479
MT‐2	Function Assay	8 µm	3 h	decreases the levels of the p65 subunit of NF‐κB	23278479
MCF-7	Function Assay	2.5-15 μM	0.5 h	causes the gradual loss of cell adhesion	23093227
HaCaT	Function Assay	5.0 μM	1 h	attenuates the TCOH-induced production of IL-6	23041168
OA chondrocytes	Function Assay	10 μM	1 h	blocks the AGE-BSA-induced gene/protein expression of GRP78 or COX-2 (p<0.05)	22982228
RAW264.7	Function Assay	15 μM	15-120 min	blocks the production of NO, PGE2, and TNF-α	22745523
RAW264.7	Growth Inhibition Assay	5-30 μM	24 h	inhibits cell growth in a dose-dependent manner	22745523
HBL6	Apoptosis Assay	0.5/5/25 μM	6/24 h	decreases cell viability and leeads to apoptosis in a dose-dependent manner	22074820
HT29	Function Assay	1-10 μM	10 h	increases HO-1 mRNA and protein expression	21620964
Ca9–22	Apoptosis Assay	10 μM	1 h	completely inhibits ALA-PDT-induced apoptosis	21138480
Ca9–22	Function Assay	10 μM	1 h	completely abrogates the ALA-PDT-induced JNK activation	21138480
A-549	Growth Inhibition Assay	10 μM	24/48 h	inhibits cell growth in a time-dependent manner	20866043
AP	Function Assay	5/10 μM	48 h	downregulates the BAD protein level a dose-dependent manner	20596645
AQ1	Function Assay	5/10 μM	48 h	downregulates the BAD protein level a dose-dependent manner	20596645
AP	Function Assay	20 μM	4/8 h	downregulates the BAD protein level a time-dependent manner	20596645
AQ1	Function Assay	20 μM	4/8 h	downregulates the BAD protein level a time-dependent manner	20596645
THP-1	Function Assay	5 μM	0.5 h	attenuates the LPS-induced p-IκBα protein by 72%	20309718
K562	Growth Inhibition Assay	2-30 μM	24 h	IC50=8 μM,inhibits cell growth in a dose-dependent manner	19646807
Jurket	Growth Inhibition Assay	2-30 μM	24 h	IC50=7.1 μM, inhibits cell growth in a dose-dependent manner	19646807
U937	Growth Inhibition Assay	2-30 μM	24 h	IC50=10.5 μM, inhibits cell growth in a dose-dependent manner	19646807
PBMC	Growth Inhibition Assay	2-30 μM	24 h	IC50=40.2 μM, inhibits cell growth in a dose-dependent manner	19646807
K562	Apoptosis Assay	2-20 μM	24 h	induces a dose-dependent apoptosis	19646807
HEK293	Function assay	20 uM	24 hrs	Inhibition of TNF-alpha stimulated NFkappaB transactivation in HEK293 cells at 20 uM measured after 24 hrs by dual luciferase reporter gene assay	27736063
RAW264.7	Function assay	20 uM	1 hr	Inhibition of LPS-induced NFkB activation in mouse RAW264.7 cells assessed as reduction in nuclear translocation of p65 at 20 uM preincubated for 1 hr followed by LPS stimulation measured after 3 hrs by Western blot method	28667873
RAW264.7	Function assay	20 uM	6 hrs	Inhibition of LPS-induced NF-kappaB activation in mouse RAW264.7 cells at 20 uM treated 30 mins before LPS challenge measured after 6 hrs by luciferase reporter gene assay	24315191
RAW264.7	Antinflammatory assay	20 uM	18 hrs	Antinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production at 20 uM treated 30 mins before LPS challenge measured after 18 hrs by Griess assay	24315191
THP1	Antinflammatory assay	5 uM	24 hrs	Antiinflammatory activity in human THP1 cells assessed as inhibition of TPA/ionomycin-induced extracellular IL-1beta level at 5 uM incubated 1 hr prior to TPA/ionomycin challenge measured after 24 hrs by ELISA	24400858
THP1	Antinflammatory assay	5 uM	24 hrs	Antiinflammatory activity in human THP1 cells assessed as inhibition of TPA/ionomycin-induced extracellular TNF-alpha production at 5 uM incubated 1 hr prior to TPA/ionomycin challenge measured after 24 hrs by ELISA	24400858
THP1	Antinflammatory assay	5 uM	24 hrs	Antiinflammatory activity in human THP1 cells assessed as inhibition of TPA/ionomycin-induced intracellular proIL-1beta level at 5 uM incubated 1 hr prior to TPA/ionomycin challenge measured after 24 hrs by ELISA	24400858
THP1	Antinflammatory assay	5 uM	24 hrs	Antiinflammatory activity in human THP1 cells assessed as inhibition of TPA/ionomycin-induced intracellular IL-1beta level at 5 uM incubated 1 hr prior to TPA/ionomycin challenge measured after 24 hrs by ELISA	24400858
RAW264.7	Function assay	0.3 ug/ml	12 hrs	Inhibition of LPS-induced NF-kB p65 phosphorylation in mouse RAW264.7 cells at 0.3 ug/ml preincubated for 12 hrs followed by LPS stimulation for 3 hrs by Western blot method	28284806
RAW264.7	Function assay	0.3 ug/ml	12 hrs	Inhibition of LPS-induced NF-kB p65 activation in mouse RAW264.7 cells at 0.3 ug/ml preincubated for 12 hrs followed by LPS stimulation for 3 hrs by DAPI staining based inverted fluorescence microscopic method	28284806
RAW264.7	Function assay	0.3 ug/ml	12 hrs	Inhibition of NF-kB p65 in mouse RAW264.7 cells assessed as reduction in LPS-induced iNOS expression at 0.3 ug/ml preincubated for 12 hrs followed by LPS stimulation for 3 hrs by Western blot method	28284806
RAW264.7	Function assay	0.3 ug/ml	12 hrs	Inhibition of NF-kB p65 in mouse RAW264.7 cells assessed as reduction in LPS-induced COX2 expression at 0.3 ug/ml preincubated for 12 hrs followed by LPS stimulation for 3 hrs by Western blot method	28284806
HEK293	Function assay	20 uM	24 hrs	Inhibition of TNFalpha-induced NFkappaB activation in HEK293 cells at 20 uM after 24 hrs by dual luciferase reporter gene assay	28873303
RAW264.7	Function assay	20 uM	2 hrs	Inhibition of NFkappaB nuclear translocation in LPS-stimulated mouse RAW264.7 cells at 20 uM pretreated for 2 hrs followed by LPS-induction by DAPI-staining based immunofluorescence microscopic method	29759725
BGC823	Function assay	5 uM	12 hrs	Inhibition of colony formation in human BGC823 cells at 5 uM treated for 12 hrs followed by incubation in drug free medium for 14 days by crystal violet staining based assay	28881286
SGC7901	Function assay	5 uM	12 hrs	Inhibition of colony formation in human SGC7901 cells at 5 uM treated for 12 hrs followed by incubation in drug free medium for 14 days by crystal violet staining based assay	28881286
RAW264.7	Function assay	10 uM	2 hrs	Inhibition of LPS-induced IL-6 mRNA expression in mouse RAW264.7 cells at 10 uM pre-incubated for 2 hrs before LPS stimulation for 24 hrs by qRT-PCR method	27038497
RAW264.7	Function assay	10 uM	2 hrs	Inhibition of LPS-induced IL-1beta mRNA expression in mouse RAW264.7 cells at 10 uM pre-incubated for 2 hrs before LPS stimulation for 24 hrs by qRT-PCR method	27038497
RAW264.7	Function assay	10 uM	2 hrs	Inhibition of LPS-induced iNOS mRNA expression in mouse RAW264.7 cells at 10 uM pre-incubated for 2 hrs before LPS stimulation for 24 hrs by qRT-PCR method	27038497
FaDu	Function Assay		2 h	inhibits p65 expression and blocks TNFα-induced TWIST expression	24220622
A549	Function Assay		1 h	inhibits LTA-induced SP-A mRNA production significantly	23031213
THP1	Cytotoxicity assay		72 hrs	Cytotoxicity against human THP1 cells assessed as reduction in cell viability after 72 hrs by MTT assay, TC50 = 1.5 μM.	28410442
RAW264.7	Function assay		6 hrs	Inhibition of LPS-induced NF-kappaB activation in mouse RAW264.7 cells treated 30 mins before LPS challenge measured after 6 hrs by luciferase reporter gene assay, IC50 = 1.72 μM.	24315191
HEK293	Function assay		6 hrs	Inhibition of TNF-alpha-induced NF-kappaB activity in HEK293 cells after 6 hrs by luciferase reporter gene assay, IC50 = 2 μM.	24533857
HEK293	Function assay		6 hrs	Inhibition of TNF-alpha-induced NF-kappaB activity in HEK293 cells after 6 hrs by luciferase reporter gene assay, IC50 = 2 μM.	24992702
HEK293	Function assay		6 hrs	Inhibition of TNFalpha-induced NF-kappaB activity (unknown origin) transfected in HEK293 cells after 6 hrs by luciferase reporter gene assay, IC50 = 2 μM.	26343828
HEK293	Function assay		6 hrs	Inhibition of TNFalpha-induced NF-kappaB activity expressed in human HEK 293 cells after 6 hrs by luciferase reporter gene assay, IC50 = 2 μM.	22850207
HEK293	Function assay		6 hrs	Inhibition of TNFalpha-induced human NFkappaB activity in HEK293 cells incubated for 6 hrs followed by compound wash out measured after 5 mins by by luciferase assay, IC50 = 2.01 μM.	22712432
HEK293	Function assay		6 hrs	Inhibition of TNFalpha-induced NFkappaB (unknown origin) activation expressed in HEK293 cells after 6 hrs by luciferase reporter gene assay, IC50 = 5 μM.	23316950
HEK293	Cytotoxicity assay			Cytotoxicity against HEK293 cells, IC50 = 3.8 μM.	24533857
HEK293	Function assay			Effect on Cdc2 expressed in HEK293 cells assessed as effect on Cdc2:Cdc25C interaction complexes in presence of camptothecin by EYFP and/or YFP Venus fragment based reporter gene assay	16680159
点击查看更多细胞系数据

生物活性

产品描述

靶点

E2-conjugating enzymes ^[6] (Cell-free assay)	USP7 ^[7] (Cell-free assay)	USP21 ^[7] (Cell-free assay)	USP6 ^[7] (Cell-free assay)	IκBα phosphorylation ^[1] (Tumor cells)
	0.19 μM	0.96 μM	1.7 μM	10 μM

体外研究(In Vitro)
体外研究活性	BAY 11-7082完全且特异性地废除 NF-κB DNA结合, 下调NF-κB-诱导的细胞因子IL-6，且诱导细胞凋亡。^[1] BAY 11-7082 (< 8 μM) 有效抑制原有的和TNFα 刺激的NF-κB荧光素酶活性，这种作用存在剂量依赖性。BAY 11-7082 (8 μM) 强抑制NCI-H1703 细胞增殖。 ^[2] Bay 11-7082 (5 μM)作用于感染HTLV-I的T细胞系，快速且有效的降低NF-κB的DNA结合力，且下调抗凋亡基因Bcl-x(L)的表达,而对另一种转录因子AP-1与DNA结合没有作用效果。Bay 11-7082诱导原代ATL细胞发生凋亡比正常的外周血单核细胞更明显，而诱导的这些细胞凋亡也与NF-κB活性的下调相关。Bay 11-7082 (5 μM) 选择性诱导感染HTLV-I的T细胞系发生细胞凋亡，且下调 cyclin D1, cyclin D2, 和 Bcl-xL的表达。 ^[3] BAY 11-7082 (100 μM)作用于小鼠海马脑片区，抑制NMDA引起的p65发生核易位，也抑制NMDA诱导的NF-κB结合力的增强。BAY 11-7082抑制NMDA毒性发生在海马脑片区的CA1区，20 μM时，对40％神经有保护作用，100 μM时，对70％神经有保护作用。^[4] 作用于脂肪组织，实验中所有浓度的BAY 11-7082都显著抑制 NF-κB p65 DNA结合活性，而作用于骨骼肌，只有50 μM 和 100 μM 的BAY 11-7082显著抑制NF-κB p65 DNA结合活性。BAY 11-7082 (100 μM) 作用于人体脂肪组织和骨骼肌，降低IKK-β蛋白。BAY 11-7082 (100 μM) 显著降低TNF-α从动物脂肪组织中释放，而 IL-6 和 IL-8 的释放显著抑制实验中BAY 11-7082的所有浓度。 BAY 11-7082 (50 μM)作用于骨骼肌，显著降低 TNF-α, IL-6, 和IL-8 的释放。^[5] 此外，BAY 11-7082还被发现抑制了 E2-conjugating酶和E3 ligase酶的活性，从而诱导了B-淋巴癌细胞和T淋巴癌细胞的死亡。 ^[6]
细胞实验	细胞系	NCI-H1703 细胞
	浓度	~8 μM
	孵育时间	12 小时
	方法	细胞在96孔板中转染siRNA，然后在完全NSCLC 培养基中培养72小时，再使用BAY 11-7082 处理12小时。细胞与[³H]胸甘温育3小时。使用自动细胞收集器将细胞收集到过滤器上，然后通过β-闪烁计数法测量放射性。
实验图片	检测方法	检测指标	实验图片	PMID
	Western blot	NF-κB p-IKKβ/ IκBα p-IRAK4 / IRAK4 NF-κB p-IKKβ/ IκBα p-IRAK4 / IRAK4		31332209
	Growth inhibition assay	Cell viability Cell viability		31332209

参考文献
https://pubmed.ncbi.nlm.nih.gov/17227230/ https://pubmed.ncbi.nlm.nih.gov/22549160/ https://pubmed.ncbi.nlm.nih.gov/16162000/ https://pubmed.ncbi.nlm.nih.gov/12176906/ https://pubmed.ncbi.nlm.nih.gov/15755516/ https://pubmed.ncbi.nlm.nih.gov/23441730/ https://pubmed.ncbi.nlm.nih.gov/25159004/ https://pubmed.ncbi.nlm.nih.gov/23846545/ + 展开

参考文献

https://pubmed.ncbi.nlm.nih.gov/17227230/
https://pubmed.ncbi.nlm.nih.gov/22549160/
https://pubmed.ncbi.nlm.nih.gov/16162000/

https://pubmed.ncbi.nlm.nih.gov/12176906/
https://pubmed.ncbi.nlm.nih.gov/15755516/
https://pubmed.ncbi.nlm.nih.gov/23441730/
https://pubmed.ncbi.nlm.nih.gov/25159004/
https://pubmed.ncbi.nlm.nih.gov/23846545/

+ 展开

化学信息&溶解度

分子量	207.25	分子式	C₁₀H₉NO₂S
CAS号	19542-67-7	SDF	Download BAY 11-7082 SDF
Smiles	CC1=CC=C(C=C1)S(=O)(=O)C=CC#N
储存条件(自收到货起)	3年-20°C粉状	储备液保存和期限建议分装储备液，避免反复冻融！	1年-80°C溶于溶剂
储存条件(自收到货起)	3年-20°C粉状	储备液保存和期限建议分装储备液，避免反复冻融！	1个月-20°C溶于溶剂

体外溶解度
批次：

DMSO : 41 mg/mL ( (197.82 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Ethanol : 10 mg/mL (48.25 mM)

Water : Insoluble

DMSO : 41 mg/mL ( (197.82 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Ethanol : 10 mg/mL (48.25 mM)

Water : Insoluble

DMSO : 41 mg/mL ( (197.82 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Ethanol : 3 mg/mL (14.47 mM)

Water : Insoluble

摩尔浓度计算器

体内溶解配方批次：现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂				动物体内配方计算器
均匀悬浊液	CMC-NA	≥5mg/ml	以 1 mL 工作液为例，取 5 mg该产品加到 1 ml CMC-NA 溶液中，混合均匀，即可得工作液浓度为 5 mg/ml的均匀悬浊液。
澄清溶液	10%DMSO 1 40%PEG300 2 5%Tween80 3 45%ddH2O 该配方已经过Selleck实验室实测。如果上述溶解方案不能满足您的需求，可联系Selleck销售提供定制化测试。	4.000mg/ml (19.30mM)	以 1 mL 工作液为例，取100μL40mg/ml的澄清DMSO储备液加到400μL PEG300中，混合均匀使其澄清；向上述体系中加入50μLTween80，混合均匀使其澄清；然后继续加入450μL ddH2O定容至 1 mL。工作液请现配现用！
均匀悬浊液	CMC-NA	≥5mg/ml	以 1 mL 工作液为例，取 5 mg该产品加到 1 ml CMC-NA 溶液中，混合均匀，即可得工作液浓度为 5 mg/ml的均匀悬浊液。
均匀悬浊液	CMC-NA	≥5mg/ml	以 1 mL 工作液为例，取 5 mg该产品加到 1 ml CMC-NA 溶液中，混合均匀，即可得工作液浓度为 5 mg/ml的均匀悬浊液。
澄清溶液	5%DMSO 1 Corn oil 该配方已经过Selleck实验室实测。如果上述溶解方案不能满足您的需求，可联系Selleck销售提供定制化测试。	8.000mg/ml (38.60mM)	以 1 mL 工作液为例，取50μL160mg/ml的澄清DMSO储备液加到950μL玉米油中，混合均匀。工作液请现配现用！
澄清溶液	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O 该配方已经过Selleck实验室实测。如果上述溶解方案不能满足您的需求，可联系Selleck销售提供定制化测试。	2.000mg/ml (9.65mM)	以 1 mL 工作液为例,取50μL40mg/ml的澄清DMSO储备液加到400μLPEG300中,混合均匀使其澄清;向上述体系中加入50μLTween80,混合均匀使其澄清;然后继续加入500μLddH2O定容至1mL。工作液请现配现用!

实验计算

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×

稀释计算器分子量计算器

动物体内配方计算器（澄清溶液）

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 μL 动物数量只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

计算结果：

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH₂O，混匀澄清。

体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。

注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

技术支持

在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题，请给我们留言。

* 必填项

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

BAY 11-7082

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