Torin 1
Torin 1是一种有效的mTORC1/2抑制剂，在无细胞试验中IC50为2 nM/10 nM；作用于mTOR比作用于PI3K选择性高1000倍。
Torin 1 Chemical Structure
CAS: 1222998-36-8
产品质控
批次：
纯度：
99.07%
99.07
常与Torin 1一起在实验中被使用的化合物
Torin 1和Rapamycin差异调节人神经母细胞瘤细胞中铁诱导的线粒体ROS的产生。
Torin 1和Verteporfin联合治疗在PDX模型中产生最大程度的持续肿瘤生长抑制。
Torin 1和Chloroquine降低BON1细胞的细胞活力并抑制细胞增殖。
Avneil-Polak S, et al. Neuroendocrinology. 2016;103(6):724-37.
Torin 1可使EGFR野生型、KRAS或NRAS、p53野生型或突变型的NSCLC细胞系对Erlotinib的敏感性提高10-20倍。
Torin 1和Cetuximab对OSCC异种移植小鼠的肿瘤体积生长有抑制作用。
Torin 1相关产品
相关信号通路图
细胞实验数据示例
| 细胞系 | 实验类型 | 给药浓度 | 孵育时间 | 活性描述 | 文献信息 |
|---|---|---|---|---|---|
| RPMI-8402 | Growth Inhibition Assay | IC50=0.07789 μM | SANGER | ||
| HAL-01 | Growth Inhibition Assay | IC50=0.07658 μM | SANGER | ||
| KM12 | Growth Inhibition Assay | IC50=0.0764 μM | SANGER | ||
| PF-382 | Growth Inhibition Assay | IC50=0.07569 μM | SANGER | ||
| LB771-HNC | Growth Inhibition Assay | IC50=0.07549 μM | SANGER | ||
| ARH-77 | Growth Inhibition Assay | IC50=0.07278 μM | SANGER | ||
| MONO-MAC-6 | Growth Inhibition Assay | IC50=0.07212 μM | SANGER | ||
| DSH1 | Growth Inhibition Assay | IC50=0.07173 μM | SANGER | ||
| MV-4-11 | Growth Inhibition Assay | IC50=0.06995 μM | SANGER | ||
| A388 | Growth Inhibition Assay | IC50=0.06893 μM | SANGER | ||
| MLMA | Growth Inhibition Assay | IC50=0.0678 μM | SANGER | ||
| MOLT-4 | Growth Inhibition Assay | IC50=0.06605 μM | SANGER | ||
| MZ1-PC | Growth Inhibition Assay | IC50=0.06598 μM | SANGER | ||
| KLE | Growth Inhibition Assay | IC50=0.06584 μM | SANGER | ||
| CTV-1 | Growth Inhibition Assay | IC50=0.06548 μM | SANGER | ||
| Becker | Growth Inhibition Assay | IC50=0.0642 μM | SANGER | ||
| K5 | Growth Inhibition Assay | IC50=0.06403 μM | SANGER | ||
| LOUCY | Growth Inhibition Assay | IC50=0.06394 μM | SANGER | ||
| SR | Growth Inhibition Assay | IC50=0.06374 μM | SANGER | ||
| SNB75 | Growth Inhibition Assay | IC50=0.06341 μM | SANGER | ||
| A3-KAW | Growth Inhibition Assay | IC50=0.06225 μM | SANGER | ||
| LS-123 | Growth Inhibition Assay | IC50=0.05942 μM | SANGER | ||
| RS4-11 | Growth Inhibition Assay | IC50=0.05938 μM | SANGER | ||
| HC-1 | Growth Inhibition Assay | IC50=0.05827 μM | SANGER | ||
| KINGS-1 | Growth Inhibition Assay | IC50=0.05803 μM | SANGER | ||
| TGBC24TKB | Growth Inhibition Assay | IC50=0.05799 μM | SANGER | ||
| SW962 | Growth Inhibition Assay | IC50=0.05763 μM | SANGER | ||
| BB30-HNC | Growth Inhibition Assay | IC50=0.0573 μM | SANGER | ||
| IST-SL2 | Growth Inhibition Assay | IC50=0.05362 μM | SANGER | ||
| NB6 | Growth Inhibition Assay | IC50=0.05271 μM | SANGER | ||
| ALL-PO | Growth Inhibition Assay | IC50=0.04956 μM | SANGER | ||
| SK-PN-DW | Growth Inhibition Assay | IC50=0.04949 μM | SANGER | ||
| HCC1599 | Growth Inhibition Assay | IC50=0.04879 μM | SANGER | ||
| NB13 | Growth Inhibition Assay | IC50=0.04776 μM | SANGER | ||
| 8-MG-BA | Growth Inhibition Assay | IC50=0.04629 μM | SANGER | ||
| COLO-684 | Growth Inhibition Assay | IC50=0.04561 μM | SANGER | ||
| TE-15 | Growth Inhibition Assay | IC50=0.04435 μM | SANGER | ||
| SF126 | Growth Inhibition Assay | IC50=0.04361 μM | SANGER | ||
| SW982 | Growth Inhibition Assay | IC50=0.04246 μM | SANGER | ||
| Daudi | Growth Inhibition Assay | IC50=0.04225 μM | SANGER | ||
| SJSA-1 | Growth Inhibition Assay | IC50=0.04188 μM | SANGER | ||
| TK10 | Growth Inhibition Assay | IC50=0.04019 μM | SANGER | ||
| LC-1F | Growth Inhibition Assay | IC50=0.03989 μM | SANGER | ||
| HCE-4 | Growth Inhibition Assay | IC50=0.03855 μM | SANGER | ||
| TE-5 | Growth Inhibition Assay | IC50=0.03854 μM | SANGER | ||
| SW954 | Growth Inhibition Assay | IC50=0.0379 μM | SANGER | ||
| CP67-MEL | Growth Inhibition Assay | IC50=0.03759 μM | SANGER | ||
| BE-13 | Growth Inhibition Assay | IC50=0.03759 μM | SANGER | ||
| BB65-RCC | Growth Inhibition Assay | IC50=0.03691 μM | SANGER | ||
| EB-3 | Growth Inhibition Assay | IC50=0.03643 μM | SANGER | ||
| NCI-H1648 | Growth Inhibition Assay | IC50=0.03622 μM | SANGER | ||
| SH-4 | Growth Inhibition Assay | IC50=0.03582 μM | SANGER | ||
| HOP-62 | Growth Inhibition Assay | IC50=0.03556 μM | SANGER | ||
| NMC-G1 | Growth Inhibition Assay | IC50=0.03448 μM | SANGER | ||
| CCRF-CEM | Growth Inhibition Assay | IC50=0.03371 μM | SANGER | ||
| TE-10 | Growth Inhibition Assay | IC50=0.03311 μM | SANGER | ||
| NTERA-S-cl-D1 | Growth Inhibition Assay | IC50=0.03226 μM | SANGER | ||
| GAK | Growth Inhibition Assay | IC50=0.03186 μM | SANGER | ||
| CESS | Growth Inhibition Assay | IC50=0.03099 μM | SANGER | ||
| ES1 | Growth Inhibition Assay | IC50=0.03097 μM | SANGER | ||
| OVCAR-4 | Growth Inhibition Assay | IC50=0.03094 μM | SANGER | ||
| EoL-1-cell | Growth Inhibition Assay | IC50=0.02996 μM | SANGER | ||
| IA-LM | Growth Inhibition Assay | IC50=0.02917 μM | SANGER | ||
| LXF-289 | Growth Inhibition Assay | IC50=0.02872 μM | SANGER | ||
| OCI-AML2 | Growth Inhibition Assay | IC50=0.02871 μM | SANGER | ||
| DEL | Growth Inhibition Assay | IC50=0.02815 μM | SANGER | ||
| MFH-ino | Growth Inhibition Assay | IC50=0.02812 μM | SANGER | ||
| ATN-1 | Growth Inhibition Assay | IC50=0.02789 μM | SANGER | ||
| Ramos-2G6-4C10 | Growth Inhibition Assay | IC50=0.0275 μM | SANGER | ||
| MC116 | Growth Inhibition Assay | IC50=0.0268 μM | SANGER | ||
| GR-ST | Growth Inhibition Assay | IC50=0.02655 μM | SANGER | ||
| A101D | Growth Inhibition Assay | IC50=0.0265 μM | SANGER | ||
| MMAC-SF | Growth Inhibition Assay | IC50=0.02633 μM | SANGER | ||
| MZ7-mel | Growth Inhibition Assay | IC50=0.02609 μM | SANGER | ||
| TE-12 | Growth Inhibition Assay | IC50=0.0255 μM | SANGER | ||
| LB1047-RCC | Growth Inhibition Assay | IC50=0.02488 μM | SANGER | ||
| GI-1 | Growth Inhibition Assay | IC50=0.02469 μM | SANGER | ||
| HD-MY-Z | Growth Inhibition Assay | IC50=0.02426 μM | SANGER | ||
| KURAMOCHI | Growth Inhibition Assay | IC50=0.02414 μM | SANGER | ||
| TE-11 | Growth Inhibition Assay | IC50=0.02372 μM | SANGER | ||
| MZ2-MEL | Growth Inhibition Assay | IC50=0.02337 μM | SANGER | ||
| NCI-H747 | Growth Inhibition Assay | IC50=0.0233 μM | SANGER | ||
| OS-RC-2 | Growth Inhibition Assay | IC50=0.02323 μM | SANGER | ||
| SK-UT-1 | Growth Inhibition Assay | IC50=0.02261 μM | SANGER | ||
| SW872 | Growth Inhibition Assay | IC50=0.02247 μM | SANGER | ||
| BV-173 | Growth Inhibition Assay | IC50=0.02203 μM | SANGER | ||
| LAMA-84 | Growth Inhibition Assay | IC50=0.02167 μM | SANGER | ||
| LC-2-ad | Growth Inhibition Assay | IC50=0.02137 μM | SANGER | ||
| ES8 | Growth Inhibition Assay | IC50=0.01844 μM | SANGER | ||
| MHH-PREB-1 | Growth Inhibition Assay | IC50=0.01818 μM | SANGER | ||
| IST-MEL1 | Growth Inhibition Assay | IC50=0.01774 μM | SANGER | ||
| NCI-H1355 | Growth Inhibition Assay | IC50=0.01646 μM | SANGER | ||
| HEL | Growth Inhibition Assay | IC50=0.0164 μM | SANGER | ||
| MOLT-16 | Growth Inhibition Assay | IC50=0.01622 μM | SANGER | ||
| no-10 | Growth Inhibition Assay | IC50=0.01584 μM | SANGER | ||
| KE-37 | Growth Inhibition Assay | IC50=0.01573 μM | SANGER | ||
| GB-1 | Growth Inhibition Assay | IC50=0.01553 μM | SANGER | ||
| CMK | Growth Inhibition Assay | IC50=0.01543 μM | SANGER | ||
| RKO | Growth Inhibition Assay | IC50=0.01533 μM | SANGER | ||
| ACN | Growth Inhibition Assay | IC50=0.015 μM | SANGER | ||
| HT-144 | Growth Inhibition Assay | IC50=0.01451 μM | SANGER | ||
| LS-411N | Growth Inhibition Assay | IC50=0.01439 μM | SANGER | ||
| SK-LMS-1 | Growth Inhibition Assay | IC50=0.01413 μM | SANGER | ||
| PSN1 | Growth Inhibition Assay | IC50=0.01409 μM | SANGER | ||
| no-11 | Growth Inhibition Assay | IC50=0.01395 μM | SANGER | ||
| KS-1 | Growth Inhibition Assay | IC50=0.01392 μM | SANGER | ||
| KGN | Growth Inhibition Assay | IC50=0.01359 μM | SANGER | ||
| A4-Fuk | Growth Inhibition Assay | IC50=0.01319 μM | SANGER | ||
| KU812 | Growth Inhibition Assay | IC50=0.01296 μM | SANGER | ||
| OPM-2 | Growth Inhibition Assay | IC50=0.0129 μM | SANGER | ||
| KALS-1 | Growth Inhibition Assay | IC50=0.01198 μM | SANGER | ||
| EW-16 | Growth Inhibition Assay | IC50=0.01192 μM | SANGER | ||
| LB996-RCC | Growth Inhibition Assay | IC50=0.01137 μM | SANGER | ||
| NALM-6 | Growth Inhibition Assay | IC50=0.01102 μM | SANGER | ||
| ES6 | Growth Inhibition Assay | IC50=0.01083 μM | SANGER | ||
| GCIY | Growth Inhibition Assay | IC50=0.01007 μM | SANGER | ||
| VA-ES-BJ | Growth Inhibition Assay | IC50=0.00984 μM | SANGER | ||
| HUTU-80 | Growth Inhibition Assay | IC50=0.00929 μM | SANGER | ||
| EVSA-T | Growth Inhibition Assay | IC50=0.00892 μM | SANGER | ||
| EW-1 | Growth Inhibition Assay | IC50=0.00749 μM | SANGER | ||
| MRK-nu-1 | Growth Inhibition Assay | IC50=0.00707 μM | SANGER | ||
| BL-41 | Growth Inhibition Assay | IC50=0.00696 μM | SANGER | ||
| RPMI-8226 | Growth Inhibition Assay | IC50=0.00651 μM | SANGER | ||
| 697 | Growth Inhibition Assay | IC50=0.00634 μM | SANGER | ||
| TGBC1TKB | Growth Inhibition Assay | IC50=0.00379 μM | SANGER | ||
| BL-70 | Growth Inhibition Assay | IC50=0.00337 μM | SANGER | ||
| RL95-2 | Growth Inhibition Assay | IC50=0.0032 μM | SANGER | ||
| L-363 | Growth Inhibition Assay | IC50=0.00284 μM | SANGER | ||
| NOS-1 | Growth Inhibition Assay | IC50=0.00242 μM | SANGER | ||
| DOHH-2 | Growth Inhibition Assay | IC50=0.00225 μM | SANGER | ||
| OCUB-M | Growth Inhibition Assay | IC50=0.00194 μM | SANGER | ||
| ETK-1 | Growth Inhibition Assay | IC50=0.000306 μM | SANGER | ||
| OMC-1 | Growth Inhibition Assay | IC50=0.07804 μM | SANGER | ||
| HT | Growth Inhibition Assay | IC50=0.08 μM | SANGER | ||
| QIMR-WIL | Growth Inhibition Assay | IC50=0.08055 μM | SANGER | ||
| KNS-42 | Growth Inhibition Assay | IC50=0.08203 μM | SANGER | ||
| BC-3 | Growth Inhibition Assay | IC50=0.08204 μM | SANGER | ||
| ES4 | Growth Inhibition Assay | IC50=0.08296 μM | SANGER | ||
| NCI-H23 | Growth Inhibition Assay | IC50=0.0844 μM | SANGER | ||
| LB2518-MEL | Growth Inhibition Assay | IC50=0.08696 μM | SANGER | ||
| LC4-1 | Growth Inhibition Assay | IC50=0.08738 μM | SANGER | ||
| SIG-M5 | Growth Inhibition Assay | IC50=0.08904 μM | SANGER | ||
| LP-1 | Growth Inhibition Assay | IC50=0.08938 μM | SANGER | ||
| TE-8 | Growth Inhibition Assay | IC50=0.09016 μM | SANGER | ||
| K-562 | Growth Inhibition Assay | IC50=0.0912 μM | SANGER | ||
| J-RT3-T3-5 | Growth Inhibition Assay | IC50=0.09153 μM | SANGER | ||
| DU-4475 | Growth Inhibition Assay | IC50=0.09165 μM | SANGER | ||
| RXF393 | Growth Inhibition Assay | IC50=0.09462 μM | SANGER | ||
| NB5 | Growth Inhibition Assay | IC50=0.09625 μM | SANGER | ||
| A704 | Growth Inhibition Assay | IC50=0.09741 μM | SANGER | ||
| SF268 | Growth Inhibition Assay | IC50=0.09825 μM | SANGER | ||
| UACC-257 | Growth Inhibition Assay | IC50=0.09833 μM | SANGER | ||
| L-428 | Growth Inhibition Assay | IC50=0.1032 μM | SANGER | ||
| ST486 | Growth Inhibition Assay | IC50=0.10606 μM | SANGER | ||
| SK-MM-2 | Growth Inhibition Assay | IC50=0.10709 μM | SANGER | ||
| COR-L279 | Growth Inhibition Assay | IC50=0.10832 μM | SANGER | ||
| JiyoyeP-2003 | Growth Inhibition Assay | IC50=0.10843 μM | SANGER | ||
| GT3TKB | Growth Inhibition Assay | IC50=0.10918 μM | SANGER | ||
| HCE-T | Growth Inhibition Assay | IC50=0.1094 μM | SANGER | ||
| SBC-1 | Growth Inhibition Assay | IC50=0.11119 μM | SANGER | ||
| KNS-81-FD | Growth Inhibition Assay | IC50=0.11273 μM | SANGER | ||
| NCI-H2126 | Growth Inhibition Assay | IC50=0.11394 μM | SANGER | ||
| EM-2 | Growth Inhibition Assay | IC50=0.11529 μM | SANGER | ||
| JAR | Growth Inhibition Assay | IC50=0.11598 μM | SANGER | ||
| NB17 | Growth Inhibition Assay | IC50=0.11652 μM | SANGER | ||
| LB647-SCLC | Growth Inhibition Assay | IC50=0.11705 μM | SANGER | ||
| CAL-148 | Growth Inhibition Assay | IC50=0.118 μM | SANGER | ||
| K052 | Growth Inhibition Assay | IC50=0.11851 μM | SANGER | ||
| KARPAS-299 | Growth Inhibition Assay | IC50=0.12233 μM | SANGER | ||
| KARPAS-422 | Growth Inhibition Assay | IC50=0.12432 μM | SANGER | ||
| LOXIMVI | Growth Inhibition Assay | IC50=0.12515 μM | SANGER | ||
| SCC-3 | Growth Inhibition Assay | IC50=0.12851 μM | SANGER | ||
| LU-139 | Growth Inhibition Assay | IC50=0.12975 μM | SANGER | ||
| COLO-320-HSR | Growth Inhibition Assay | IC50=0.13086 μM | SANGER | ||
| ONS-76 | Growth Inhibition Assay | IC50=0.1313 μM | SANGER | ||
| U-266 | Growth Inhibition Assay | IC50=0.13245 μM | SANGER | ||
| HH | Growth Inhibition Assay | IC50=0.13535 μM | SANGER | ||
| BB49-HNC | Growth Inhibition Assay | IC50=0.13587 μM | SANGER | ||
| NCI-SNU-5 | Growth Inhibition Assay | IC50=0.13596 μM | SANGER | ||
| C2BBe1 | Growth Inhibition Assay | IC50=0.13746 μM | SANGER | ||
| ML-2 | Growth Inhibition Assay | IC50=0.13953 μM | SANGER | ||
| SCH | Growth Inhibition Assay | IC50=0.13974 μM | SANGER | ||
| NCI-H82 | Growth Inhibition Assay | IC50=0.14104 μM | SANGER | ||
| MDA-MB-134-VI | Growth Inhibition Assay | IC50=0.14252 μM | SANGER | ||
| SK-N-DZ | Growth Inhibition Assay | IC50=0.14719 μM | SANGER | ||
| HL-60 | Growth Inhibition Assay | IC50=0.1492 μM | SANGER | ||
| LB373-MEL-D | Growth Inhibition Assay | IC50=0.15539 μM | SANGER | ||
| LB831-BLC | Growth Inhibition Assay | IC50=0.16076 μM | SANGER | ||
| SK-NEP-1 | Growth Inhibition Assay | IC50=0.16101 μM | SANGER | ||
| IMR-5 | Growth Inhibition Assay | IC50=0.16483 μM | SANGER | ||
| Calu-6 | Growth Inhibition Assay | IC50=0.16537 μM | SANGER | ||
| NCI-H1882 | Growth Inhibition Assay | IC50=0.16728 μM | SANGER | ||
| NCI-H446 | Growth Inhibition Assay | IC50=0.16891 μM | SANGER | ||
| GI-ME-N | Growth Inhibition Assay | IC50=0.1802 μM | SANGER | ||
| MPP-89 | Growth Inhibition Assay | IC50=0.18121 μM | SANGER | ||
| CGTH-W-1 | Growth Inhibition Assay | IC50=0.18436 μM | SANGER | ||
| MEG-01 | Growth Inhibition Assay | IC50=0.18628 μM | SANGER | ||
| NCI-H748 | Growth Inhibition Assay | IC50=0.18911 μM | SANGER | ||
| COLO-829 | Growth Inhibition Assay | IC50=0.20673 μM | SANGER | ||
| GOTO | Growth Inhibition Assay | IC50=0.2073 μM | SANGER | ||
| EW-3 | Growth Inhibition Assay | IC50=0.21718 μM | SANGER | ||
| KY821 | Growth Inhibition Assay | IC50=0.22045 μM | SANGER | ||
| CTB-1 | Growth Inhibition Assay | IC50=0.22228 μM | SANGER | ||
| TALL-1 | Growth Inhibition Assay | IC50=0.22921 μM | SANGER | ||
| NCI-H209 | Growth Inhibition Assay | IC50=0.22962 μM | SANGER | ||
| IST-MES1 | Growth Inhibition Assay | IC50=0.2351 μM | SANGER | ||
| DMS-114 | Growth Inhibition Assay | IC50=0.23993 μM | SANGER | ||
| SNU-C2B | Growth Inhibition Assay | IC50=0.24316 μM | SANGER | ||
| DG-75 | Growth Inhibition Assay | IC50=0.26195 μM | SANGER | ||
| D-336MG | Growth Inhibition Assay | IC50=0.26557 μM | SANGER | ||
| KMOE-2 | Growth Inhibition Assay | IC50=0.27538 μM | SANGER | ||
| NB1 | Growth Inhibition Assay | IC50=0.27926 μM | SANGER | ||
| RH-1 | Growth Inhibition Assay | IC50=0.27989 μM | SANGER | ||
| TE-1 | Growth Inhibition Assay | IC50=0.28479 μM | SANGER | ||
| RPMI-6666 | Growth Inhibition Assay | IC50=0.2884 μM | SANGER | ||
| TE-9 | Growth Inhibition Assay | IC50=0.28985 μM | SANGER | ||
| BT-474 | Growth Inhibition Assay | IC50=0.29334 μM | SANGER | ||
| COLO-668 | Growth Inhibition Assay | IC50=0.29429 μM | SANGER | ||
| NCI-H1581 | Growth Inhibition Assay | IC50=0.29689 μM | SANGER | ||
| MN-60 | Growth Inhibition Assay | IC50=0.29905 μM | SANGER | ||
| L-540 | Growth Inhibition Assay | IC50=0.30081 μM | SANGER | ||
| MOLT-13 | Growth Inhibition Assay | IC50=0.30183 μM | SANGER | ||
| NCI-SNU-16 | Growth Inhibition Assay | IC50=0.30205 μM | SANGER | ||
| NCI-H64 | Growth Inhibition Assay | IC50=0.30331 μM | SANGER | ||
| CP66-MEL | Growth Inhibition Assay | IC50=0.31415 μM | SANGER | ||
| NB69 | Growth Inhibition Assay | IC50=0.32188 μM | SANGER | ||
| NCI-H2196 | Growth Inhibition Assay | IC50=0.33036 μM | SANGER | ||
| MHH-CALL-2 | Growth Inhibition Assay | IC50=0.33468 μM | SANGER | ||
| HCC2157 | Growth Inhibition Assay | IC50=0.33954 μM | SANGER | ||
| LS-1034 | Growth Inhibition Assay | IC50=0.36388 μM | SANGER | ||
| BOKU | Growth Inhibition Assay | IC50=0.37199 μM | SANGER | ||
| GDM-1 | Growth Inhibition Assay | IC50=0.37234 μM | SANGER | ||
| TGW | Growth Inhibition Assay | IC50=0.37453 μM | SANGER | ||
| SNU-C1 | Growth Inhibition Assay | IC50=0.38215 μM | SANGER | ||
| LS-513 | Growth Inhibition Assay | IC50=0.39071 μM | SANGER | ||
| NOMO-1 | Growth Inhibition Assay | IC50=0.39971 μM | SANGER | ||
| MS-1 | Growth Inhibition Assay | IC50=0.40177 μM | SANGER | ||
| EKVX | Growth Inhibition Assay | IC50=0.44461 μM | SANGER | ||
| SK-MEL-2 | Growth Inhibition Assay | IC50=0.464 μM | SANGER | ||
| HCC1187 | Growth Inhibition Assay | IC50=0.46768 μM | SANGER | ||
| HCC2218 | Growth Inhibition Assay | IC50=0.47227 μM | SANGER | ||
| NCI-H187 | Growth Inhibition Assay | IC50=0.4784 μM | SANGER | ||
| DB | Growth Inhibition Assay | IC50=0.50055 μM | SANGER | ||
| NCI-H69 | Growth Inhibition Assay | IC50=0.50247 μM | SANGER | ||
| NCI-H1694 | Growth Inhibition Assay | IC50=0.50697 μM | SANGER | ||
| CA46 | Growth Inhibition Assay | IC50=0.50917 μM | SANGER | ||
| EHEB | Growth Inhibition Assay | IC50=0.51193 μM | SANGER | ||
| NCCIT | Growth Inhibition Assay | IC50=0.54321 μM | SANGER | ||
| NCI-H720 | Growth Inhibition Assay | IC50=0.54962 μM | SANGER | ||
| LU-134-A | Growth Inhibition Assay | IC50=0.55296 μM | SANGER | ||
| MC-CAR | Growth Inhibition Assay | IC50=0.55431 μM | SANGER | ||
| NEC8 | Growth Inhibition Assay | IC50=0.56172 μM | SANGER | ||
| NCI-H524 | Growth Inhibition Assay | IC50=0.58748 μM | SANGER | ||
| NCI-H1963 | Growth Inhibition Assay | IC50=0.6258 μM | SANGER | ||
| SU-DHL-1 | Growth Inhibition Assay | IC50=0.64526 μM | SANGER | ||
| NCI-H1522 | Growth Inhibition Assay | IC50=0.64883 μM | SANGER | ||
| NCI-H1155 | Growth Inhibition Assay | IC50=0.6535 μM | SANGER | ||
| D-263MG | Growth Inhibition Assay | IC50=0.66101 μM | SANGER | ||
| TE-6 | Growth Inhibition Assay | IC50=0.68104 μM | SANGER | ||
| NCI-H1417 | Growth Inhibition Assay | IC50=0.69711 μM | SANGER | ||
| NCI-H510A | Growth Inhibition Assay | IC50=0.77236 μM | SANGER | ||
| KARPAS-45 | Growth Inhibition Assay | IC50=0.78235 μM | SANGER | ||
| IST-SL1 | Growth Inhibition Assay | IC50=0.85825 μM | SANGER | ||
| COLO-824 | Growth Inhibition Assay | IC50=0.87835 μM | SANGER | ||
| NCI-H526 | Growth Inhibition Assay | IC50=0.90958 μM | SANGER | ||
| NCI-H719 | Growth Inhibition Assay | IC50=0.96657 μM | SANGER | ||
| SF539 | Growth Inhibition Assay | IC50=0.98396 μM | SANGER | ||
| NKM-1 | Growth Inhibition Assay | IC50=0.98663 μM | SANGER | ||
| A253 | Growth Inhibition Assay | IC50=0.98885 μM | SANGER | ||
| TE-441-T | Growth Inhibition Assay | IC50=1.0435 μM | SANGER | ||
| COR-L88 | Growth Inhibition Assay | IC50=1.11354 μM | SANGER | ||
| CPC-N | Growth Inhibition Assay | IC50=1.15888 μM | SANGER | ||
| NCI-H2107 | Growth Inhibition Assay | IC50=1.17261 μM | SANGER | ||
| ECC4 | Growth Inhibition Assay | IC50=1.18693 μM | SANGER | ||
| NCI-H2141 | Growth Inhibition Assay | IC50=1.19299 μM | SANGER | ||
| NCI-H1770 | Growth Inhibition Assay | IC50=1.32928 μM | SANGER | ||
| NCI-SNU-1 | Growth Inhibition Assay | IC50=1.3304 μM | SANGER | ||
| LU-165 | Growth Inhibition Assay | IC50=1.45467 μM | SANGER | ||
| Raji | Growth Inhibition Assay | IC50=1.47259 μM | SANGER | ||
| EW-24 | Growth Inhibition Assay | IC50=1.54619 μM | SANGER | ||
| KP-N-RT-BM-1 | Growth Inhibition Assay | IC50=1.56551 μM | SANGER | ||
| SK-MEL-1 | Growth Inhibition Assay | IC50=1.64877 μM | SANGER | ||
| SCLC-21H | Growth Inhibition Assay | IC50=1.66651 μM | SANGER | ||
| SIMA | Growth Inhibition Assay | IC50=1.68233 μM | SANGER | ||
| NCI-H1304 | Growth Inhibition Assay | IC50=1.70765 μM | SANGER | ||
| REH | Growth Inhibition Assay | IC50=1.71078 μM | SANGER | ||
| KP-N-YS | Growth Inhibition Assay | IC50=1.93551 μM | SANGER | ||
| DMS-153 | Growth Inhibition Assay | IC50=1.9759 μM | SANGER | ||
| NB10 | Growth Inhibition Assay | IC50=2.20878 μM | SANGER | ||
| NCI-H345 | Growth Inhibition Assay | IC50=2.61905 μM | SANGER | ||
| SK-N-FI | Growth Inhibition Assay | IC50=2.75381 μM | SANGER | ||
| NCI-H1092 | Growth Inhibition Assay | IC50=2.95394 μM | SANGER | ||
| KM-H2 | Growth Inhibition Assay | IC50=3.65396 μM | SANGER | ||
| KG-1 | Growth Inhibition Assay | IC50=3.71688 μM | SANGER | ||
| D-283MED | Growth Inhibition Assay | IC50=3.7587 μM | SANGER | ||
| LU-65 | Growth Inhibition Assay | IC50=4.11485 μM | SANGER | ||
| MSTO-211H | Growth Inhibition Assay | IC50=4.18898 μM | SANGER | ||
| NCI-H2081 | Growth Inhibition Assay | IC50=4.29174 μM | SANGER | ||
| D-247MG | Growth Inhibition Assay | IC50=4.43191 μM | SANGER | ||
| P31-FUJ | Growth Inhibition Assay | IC50=4.45019 μM | SANGER | ||
| DMS-79 | Growth Inhibition Assay | IC50=4.93183 μM | SANGER | ||
| KASUMI-1 | Growth Inhibition Assay | IC50=4.97509 μM | SANGER | ||
| JVM-3 | Growth Inhibition Assay | IC50=5.25014 μM | SANGER | ||
| LAN-6 | Growth Inhibition Assay | IC50=5.51736 μM | SANGER | ||
| DJM-1 | Growth Inhibition Assay | IC50=5.56029 μM | SANGER | ||
| JVM-2 | Growth Inhibition Assay | IC50=5.67092 μM | SANGER | ||
| U-87-MG | Growth Inhibition Assay | IC50=5.89964 μM | SANGER | ||
| SHP-77 | Growth Inhibition Assay | IC50=5.94928 μM | SANGER | ||
| RPMI-8866 | Growth Inhibition Assay | IC50=6.27013 μM | SANGER | ||
| ES7 | Growth Inhibition Assay | IC50=6.39924 μM | SANGER | ||
| WSU-NHL | Growth Inhibition Assay | IC50=6.51609 μM | SANGER | ||
| CAS-1 | Growth Inhibition Assay | IC50=7.09749 μM | SANGER | ||
| MHH-NB-11 | Growth Inhibition Assay | IC50=8.23152 μM | SANGER | ||
| ES5 | Growth Inhibition Assay | IC50=9.52286 μM | SANGER | ||
| HDLM-2 | Growth Inhibition Assay | IC50=9.95902 μM | SANGER | ||
| NCI-H2171 | Growth Inhibition Assay | IC50=10.5927 μM | SANGER | ||
| LNCaP-Clone-FGC | Growth Inhibition Assay | IC50=11.3718 μM | SANGER | ||
| NCI-H1395 | Growth Inhibition Assay | IC50=12.2699 μM | SANGER | ||
| NCI-H1436 | Growth Inhibition Assay | IC50=13.3195 μM | SANGER | ||
| RCC10RGB | Growth Inhibition Assay | IC50=13.3221 μM | SANGER | ||
| SW684 | Growth Inhibition Assay | IC50=13.9797 μM | SANGER | ||
| NCI-H1650 | Growth Inhibition Assay | IC50=15.0447 μM | SANGER | ||
| EW-13 | Growth Inhibition Assay | IC50=15.2021 μM | SANGER | ||
| EC-GI-10 | Growth Inhibition Assay | IC50=15.8644 μM | SANGER | ||
| CHP-126 | Growth Inhibition Assay | IC50=17.1918 μM | SANGER | ||
| NCI-H226 | Growth Inhibition Assay | IC50=18.0403 μM | SANGER | ||
| NCI-H1838 | Growth Inhibition Assay | IC50=20.1848 μM | SANGER | ||
| EW-11 | Growth Inhibition Assay | IC50=24.2718 μM | SANGER | ||
| EB2 | Growth Inhibition Assay | IC50=31.5318 μM | SANGER | ||
| D-502MG | Growth Inhibition Assay | IC50=43.9229 μM | SANGER | ||
| U-698-M | Growth Inhibition Assay | IC50=48.8671 μM | SANGER | ||
| ES3 | Growth Inhibition Assay | IC50=49.3893 μM | SANGER | ||
| PC3 | Function assay | Inhibition of PI3Kalpha in human PC3 cells expressing Akt1 S473D mutant assessed as phosphorylation of Akt Thr308 by immunoblotting, EC50 = 1.8 μM. | 21322566 | ||
| TC32 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for TC32 cells | 29435139 | ||
| A673 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells | 29435139 | ||
| BT-37 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells | 29435139 | ||
| OHS-50 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells | 29435139 | ||
| Rh41 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells | 29435139 | ||
| SJ-GBM2 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells | 29435139 | ||
| SK-N-MC | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells | 29435139 | ||
| NB-EBc1 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells | 29435139 | ||
| SK-N-MC | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-MC cells | 29435139 | ||
| TC32 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for TC32 cells | 29435139 | ||
| 点击查看更多细胞系数据 | |||||
生物活性
| 产品描述 | Torin 1是一种有效的mTORC1/2抑制剂，在无细胞试验中IC50为2 nM/10 nM；作用于mTOR比作用于PI3K选择性高1000倍。 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 靶点 |
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| 体外研究(In Vitro) | ||||
| 体外研究活性 | Torin1在2和10 nM浓度下分别抑制mTORC1 和mTORC2底物的磷酸化。此外，Torin1对mTOR比对PI3K (EC50 = 1800 nM)的选择性高1000倍，比对450种其它蛋白激酶的结合选择性高100倍。[1] [2] Torin1通过耐rapamycin机制引起细胞周期阻滞，并且其不依赖于mTORC2。Torin1比rapamycin更完全地干扰mTORC1依赖表现型。Cap依赖性翻译需要mTORC1耐Rapamycin的功能。[1]在近期的一项研究中，据报道Torin1通过活化人内分泌细胞系BON 中MEK/ERK/c-Jun通路，能够增加神经降压素分泌和基因表达。[3] | |||
|---|---|---|---|---|
| 激酶实验 | mTORC1和 mTORC2体外激酶试验 | |||
| 为产生可溶性mTORC1，HEK-293T细胞系，稳定表达N端FLAG标记的Raptor，使用水泡性口炎病毒G-假型病毒MSCV逆转录酶病毒产生。对于mTORC2，产生相似的HeLa细胞，能够稳定表达N端FLAG-标记的Protor-1。两个复合物通过在50 mm HEPES，pH 7.4，10 mm 焦磷酸钠，10 mm β-甘油磷酸钠，100 mm NaCl，2 mm EDTA，0.3% CHAPS中裂解细胞纯化。细胞在4 °C下裂解30分钟，不溶解部分通过以13,000 rpm微量离心10分钟移除。上清液与FLAG-M2单克隆抗体-琼脂糖培育1小时，然后用裂解缓冲液洗涤3次，用包含终浓度0.5 M NaCl的裂解缓冲液洗涤1次。纯化的mTORC1用100 μg/mL 3×FLAG多肽在50 mm HEPES，pH 7.4，100 mm NaCl中洗脱。将洗出液等分收集，储存在-80 °C。纯化底物S6K1 和 Akt1。激酶试验在30 °C，由激酶缓冲液(25 mm HEPES，pH 7.4，50 mm KCl，10 mm MgCl2，500 μm ATP)和150 ng 灭活的S6K1或Akt1底物组成的20 μL终体积混合物中进行。反应通过加入80 μL样品缓冲液停止，并沸煮5分钟。随后，样品通过SDS-PAGE和免疫印迹法分析。 | ||||
| 细胞实验 | 细胞系 | MEFs | ||
| 浓度 | ~250 nM | |||
| 孵育时间 | 4天 | |||
| 方法 | 细胞活性通过CellTiter-Glo发光法细胞活性测定评估。在第0天，细胞以500细胞每孔接种到96孔板，并生长过夜。第1天，细胞用适当的化合物处理，随后在第3-5天分析。对于分析，板在室温下培育60分钟，将50 μL CellTiter-Glo试剂加入每孔中，板在定轨摇床上混合12分钟。发光在标准平板光度计上定量。 | |||
| 实验图片 | 检测方法 | 检测指标 | 实验图片 | PMID |
| Western blot | Vinculin / p-S6 p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1 LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236) ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b |
|
28757207 | |
| Immunofluorescence | lipin-1 |
|
28347999 | |
| Growth inhibition assay | Cell viability |
|
28757207 | |
| 体内研究(In Vivo) | ||
| 体内研究活性 | Torin1在20 mg/kg剂量下，在U87MG异种移植模型中是有效的，并且在肿瘤和外周组织中对mTOR下游效应蛋白表现出良好的药效学抑制作用。[2] | |
|---|---|---|
| 动物实验 | Animal Models | U87MG 异种移植物模型 |
| Dosages | 20 mg/kg | |
| Administration | 腹腔注射给药，每天一次 | |
化学信息&溶解度
| 分子量 | 607.62 | 分子式 | C35H28F3N5O2 |
| CAS号 | 1222998-36-8 | SDF | Download Torin 1 SDF |
| Smiles | CCC(=O)N1CCN(CC1)C2=C(C=C(C=C2)N3C(=O)C=CC4=CN=C5C=CC(=CC5=C43)C6=CC7=CC=CC=C7N=C6)C(F)(F)F | ||
| 储存条件(自收到货起) | |||
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体外溶解度 |
DMSO : 3 mg/mL ( (4.93 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO) Water : Insoluble Ethanol : Insoluble |
摩尔浓度计算器 |
|
体内溶解度 现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂 |
动物体内配方计算器 | ||||
实验计算
动物体内配方计算器（澄清溶液）
第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）
mg/kg
g
μL
只
第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）
% DMSO
%
% Tween 80
% ddH2O
%DMSO
%
计算结果：
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；
体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH2O，混匀澄清。
体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。
注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
技术支持
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常见问题及建议解决方法
问题 1:
How to re-constitute the inhibitor for i.p. administration?
回答：
S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.
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