Novobiocin Sodium (NSC 2382)
别名: Albamycin,Cathomycin,NSC 2382 中文名称：新生霉素钠
Novobiocin Sodium (NSC 2382, Albamycin, Cathomycin)是一种非常有效的细菌DNA旋转酶和人体有机阴离子转运体，作用于hOAT1，hOAT3和hOAT4，Ki分别为14.87±0.40μM, 4.77±1.12μM 和 90.50±7.50μM。
Novobiocin Sodium (NSC 2382) Chemical Structure
CAS: 1476-53-5
产品质控
批次：
纯度：
99.91%
99.91
Novobiocin Sodium (NSC 2382)相关产品
细胞实验数据示例
| 细胞系 | 实验类型 | 给药浓度 | 孵育时间 | 活性描述 | 文献信息 |
|---|---|---|---|---|---|
| MELN | Function assay | 200 uM | 18 hrs | Inhibition of estradiol-induced ER-mediated transcription in MELN cells by measuring luciferase activity at 200 uM after 18 hrs | 17979263 |
| yeast PP30 | Function assay | 100 uM | 3 hrs | Inhibition of human HSP90alpha expressed in yeast PP30 cells co-expressing HSF-lacZ reporter assessed as inhibition of heat stress-induced response at 100 uM after 3 hrs | 21129982 |
| SKBR3 | Function assay | 1000 uM | 18 hrs | Inhibition of HSP90 in human SKBR3 cells assessed as aggregation of intracellular HER2 at 1000 uM after 18 hrs by DAPI staining-based immunofluorescence microscopic analysis | 23859777 |
| SH-SY5Y | Neuroprotection assay | 10 nM | Neuroprotection against beta-amyloid peptide 1-42-induced toxicity in retinoic acid-differentiated human SH-SY5Y cells assessed as lactate dehydrogenase release at 10 nM | 19138859 | |
| BL21 (DE3) pLysS | Function assay | 100 mins | Inhibition of 6-His-tagged Mycobacterium smegmatis GyrB expressed in Escherichia coli BL21 (DE3) pLysS cells incubated for 100 mins in presence of ATP by malachite green dye based ATP assay, IC50=0.18μM | 25129171 | |
| BL21 (DE3) pLysS | Function assay | 100 mins | Inhibition of Mycobacterium smegmatis GyrB ATPase activity expressed in Escherichia coli BL21 (DE3) pLysS cells after 100 mins by inorganic phosphate release detection based malachite green reagent assay, IC50=0.18μM | 25801151 | |
| BL21 (DE3) pLysS | Function assay | 100 mins | Inhibition of Mycobacterium smegmatis DNA gyrase B expressed in Escherichia coli BL21 (DE3) pLysS cells assessed as reduction in ATPase activity incubated for 100 mins by inorganic phosphate detection assay, IC50=0.18μM | 26318054 | |
| BL21 (DE3) pLysS | Function assay | 120 mins | Inhibition of Mycobacterium smegmatis 155 6His-tagged DNA gyrase B catalytic domain ATPase activity expressed in Escherichia coli BL21 (DE3) pLysS cells assessed as inorganic phosphate release after 120 mins in presence of ATP by malachite green dye based, IC50=0.046μM | 27371925 | |
| MCF7-MX | Function assay | TP_TRANSPORTER: inhibition of mitoxantrone efflux in BCRP-expressing MCF7-MX cells, IC50=25μM | 16460798 | ||
| SH-SY5Y | Neuroprotection assay | Neuroprotection against beta-amyloid peptide 1-42-induced toxicity in human SH-SY5Y cells assessed as lactate dehydrogenase release, EC50=0.04839μM | 19138859 | ||
| SH-SY5Y | Neuroprotection assay | Neuroprotection against beta-amyloid peptide 1-42-induced toxicity in retinoic acid-differentiated human SH-SY5Y cells assessed as lactate dehydrogenase release | 19138859 | ||
| LNCaP-LN3 | Antiproliferative assay | Antiproliferative activity against human LNCaP-LN3 cells, IC50=1.05μM | 21129982 | ||
| Sf9 | Function assay | Inhibition of Escherichia coli His6-tagged ParC/ParE expressed in baculovirus-infected Sf9 cells by gel electrophoresis, IC50=0.21μM | 21235241 | ||
| 点击查看更多细胞系数据 | |||||
生物活性
| 产品描述 | Novobiocin Sodium (NSC 2382, Albamycin, Cathomycin)是一种非常有效的细菌DNA旋转酶和人体有机阴离子转运体，作用于hOAT1，hOAT3和hOAT4，Ki分别为14.87±0.40μM, 4.77±1.12μM 和 90.50±7.50μM。 | ||
|---|---|---|---|
| 靶点 |
|
| 体外研究(In Vitro) | ||||
| 体外研究活性 | Novobiocin和Hsp90结合，改变分子伴侣对geldanamycin和radicicol的亲和力，造成关键调节Hsp90依赖性激酶，包括V形的Src，RAF-1，和P185（ErbB2）在体内外的损耗。Novobiocin干扰共伴侣Hsc70和p23对Hsp90的结合。[1] Novobiocin以浓度依赖性方式特异性地抑制血红素调节eIF2alpha激酶（HRI）的成熟。Novobiocin诱导Hsp90和CDC37从未成熟HRI解离，而Hsp90的共分子伴侣P23，FKBP52，和蛋白磷酸酶5仍然与未成熟HRI相结合。[2] Novobiocin引起特征形态和生化变化，导致细胞死亡，显示出后生动物细胞凋亡。[3] Novobiocin，一个HSP90抑制剂，可降低SMYD3的表达并剂量依赖性抑制MDA-MB-231人乳腺癌细胞的增殖和迁移Novobiocin能抑制乳腺癌细胞的迁移，这可能涉及SMYD3的下调。[4] Novobiocin，一个aminocoumarin抗生素，干扰了热休克蛋白90和缺氧诱导因子依赖的基因表达，从而损害了细胞的存活。Novobiocin（500 μM）导致[Ca（2 +）] i的显著增加，减少前向散射，增加膜联蛋白-V结合并增强ceramide的形成。Novobiocin刺激红细胞死亡，至少部分是因为细胞外Ca（2+）的进入和ceramide的形成。[5] |
|||
|---|---|---|---|---|
化学信息&溶解度
| 分子量 | 634.61 | 分子式 | C31H35N2O11.Na |
| CAS号 | 1476-53-5 | SDF | Download Novobiocin Sodium (NSC 2382) SDF |
| Smiles | CC1=C(C=CC2=C1OC(=O)C(=C2O)NC(=O)C3=CC(=C(C=C3)[O-])CC=C(C)C)OC4C(C(C(C(O4)(C)C)OC)OC(=O)N)O.[Na+] | ||
| 储存条件(自收到货起) | |||
|
体外溶解度 |
DMSO : 100 mg/mL ( (157.57 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO) Water : 100 mg/mL (157.57 mM) Ethanol : 100 mg/mL (157.57 mM) |
摩尔浓度计算器 |
|
体内溶解度 现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂 |
动物体内配方计算器 | ||||
实验计算
动物体内配方计算器（澄清溶液）
第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）
mg/kg
g
μL
只
第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）
% DMSO
%
% Tween 80
% ddH2O
%DMSO
%
计算结果：
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；
体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH2O，混匀澄清。
体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。
注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
技术支持
在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。
如果有其他问题，请给我们留言。
* 必填项
Tags: buy Novobiocin Sodium (NSC 2382) | Novobiocin Sodium (NSC 2382) supplier | purchase Novobiocin Sodium (NSC 2382) | Novobiocin Sodium (NSC 2382) cost | Novobiocin Sodium (NSC 2382) manufacturer | order Novobiocin Sodium (NSC 2382) | Novobiocin Sodium (NSC 2382) distributor
