ATP disodium

别名: Adenosine-Triphosphate disodium, Adenosine 5'-triphosphate 中文名称：ATP二钠, 三磷酸腺苷二钠

目录号：S1985 Purity: 99.79%

ATP disodium是三磷酸腺苷的钠盐形式，是一种多功能的三磷酸核苷。

ATP disodium Chemical Structure

CAS: 987-65-5

规格	价格	库存
50mg	783.91	现货
1g	5487.3	现货
更大包装有超大折扣
400-668-6834 info@selleck.cn

产品质控

批次：纯度： 99.79%

99.79

ATP disodium相关产品

相关产品	BAPTA-AM Rat IgG2b isotype control-InVivo Blebbistatin Methyl-β-cyclodextrin (MβCD) AP-III-a4 (ENOblock) Carbazochrome sodium sulfonate (AC-17) Ursolic Acid Osthole Anti-Flag-tag Mouse Recombinant mAb NAD+ 4-Methylumbelliferone (4-MU) Dioscin Ethidium bromide Pitstop 2 Sesamin Bilobalide Formononetin Hesperidin Azeliragon (TTP488) L-Mimosine Ademetionine Carbenoxolone Sodium GO-203 Vitexin N6022 Dp44mT Chlorogenic Acid Sphingosine Chrysin Coenzyme Q10 (CoQ10) Spermine Tetrahydrochloride 2-NBDG 2-NBDG D-Lin-MC3-DMA (MC3) Morin Hydrate Ethylenediaminetetraacetic acid Silibinin (NSC 651520) (+)-Fangchinoline Bergenin Spermine NB 598 DMNQ (20S)Ginsenoside Rg2 Iba1 Rabbit Recombinant mAb Farrerol EGCG Octaacetate D-(+)-Trehalose dihydrate Reboxetine mesylate E7820 MYLS22 Hydroxytyrosol Evofosfamide (TH-302) Bromhexine HCl 5-hydroxytryptophan (5-HTP) Inosine D-Mannose Anethole trithione Sodium Nitroprusside Dihydrate 2,4-Pyridinedicarboxylic acid GSK805 ADP Acipimox GPX4 Rabbit Recombinant mAb Curcumin analog C1 Trimetazidine 2,2,2-Tribromoethanol Sulfopin N-Acetylneuraminic acid Quinacrine Dihydrochloride Dihydrate L-methionine Stigmasterol Oleanonic Acid Substance P TFA Achyranthes bidentata root Extract Ginsenoside Rg3 Phyllanthus Urinaria Extract NSI-189 SR8278 Lycopene 1-Methyl-3-nitro-1-nitrosoguanidine (MNNG) Ureidosuccinic acid Tea polyphenol Fructose (+)-JQ1 carboxylic acid Ligustilide Polybrene (Hexadimethrine Bromide) Pinoresinol diglucoside 4-HNE (4-Hydroxynonenal) Iron Dextran Sodium Picosulfate IM-54 Disufenton sodium Cytokeratin 5 Rabbit Recombinant mAb Troxipide Orcinol glucoside Diludine Laminin beta 1 Rabbit Recombinant mAb 20R-Camptothecin (+)-Usniacin 3-Indolebutyric acid (IBA) HEPES Cremophor EL BMS-986176 DTT (Dithiothreitol) Esculin Methyl Eugenol Polyinosinic acid-polycytidylic acid DDIT3 Rabbit Recombinant mAb NQO1 Rabbit Recombinant mAb L-(-)-Glucose 1-Hydroxy-2-naphthoic acid Azomycin 3-Hydroxybenzoic acid Emlenoflast (MCC-7840) β-Alanine β-Sitosterol Cytokeratin 15 Rabbit Recombinant mAb Rat IgG1 isotype control-InVivo ATP5A1 Rabbit Recombinant mAb Aminomalonic acid Helecin BRM/BRG1 ATP Inhibitor-1 WDR5 Rabbit Recombinant mAb 23-Hydroxybetulinic acid PZ-2891 MCM5 Rabbit Recombinant mAb Toosendanin 1,2-Propanediol Epibrassinolide Cyanidin-3-O-glucoside chloride Dihydrotanshinone I Ferritin Rabbit Recombinant mAb Sodium Pyruvate 1-Methyl-2-pyrrolidone Itaconic acid FH1 Cinnamic acid Molsidomine Calcium D-saccharate tetrahydrate Creatine monohydrate DL-Dopa FPH1 5'-Adenylic acid Acetamide ML329 PKC theta Rabbit Recombinant mAb Pterostilbene Kinetin Acetyl Resveratrol GSK4112 LTI-291 Plantamajoside Anhydroicaritin BZ1 Phloretic acid Anemoside B4 EGTA Sucralose 6-Chloropurine Glucosamine hydrochloride Dynamin 2 Rabbit Recombinant mAb Sinoacutine 2,4-Dihydroxyacetophenone Stearic acid Stevioside Morin PMPA tetrasodium salt L-Hydroorotic acid Irigenin Behenic Acid TSG101 Rabbit Recombinant mAb CTX-0294885 Fudosteine (R)-(-)-JQ1 Enantiomer 2'-Deoxyguanosine monohydrate L-Tryptophanamide Hydrochloride β-Cyclodextrin 3-Indolepropionic acid DL-Citrulline Sclareol Methyl protocatechuate Sodium nitroprusside N-Nitrosodiethylamine Deoxyaconitine N-Acetyl-L-methionine Sinapine thiocyanate Sodium succinate Senkyunolide A L-Tryptophan Swertiamarin Octyl gallate Brassinazole	点击展开
相关化合物库	FDA药物库天然产物库已知活性药物库-I 生物活性库Ⅱ 多样性化合物母核库	点击展开

细胞实验数据示例

细胞系	实验类型	给药浓度	孵育时间	活性描述	文献信息
MDCK	Function assay	10 uM		Induction of intracellular calcium level in MDCK cells at 10 uM by Fura-2 in calcium free medium in presence of 10 uM U73343 inactive analogue of U73122 phospholipase C inhibitor	23163425
MDCK	Function assay	10 uM		Induction of intracellular calcium level in MDCK cells assessed as net peak value at 10 uM by Fura-2 in calcium free medium, Activity=0.132μM.	23163425
HEK293	Function assay	1 uM		Agonist activity at ALX/FPR2 (unknown origin) expressed in HEK293 cells co-expressing Galphaq assessed as induction of intracellular calcium accumulation at 1 uM by Fluo-4 dye based fluorescence spectrophotometric assay	30419493
HEK293	Function assay	1 uM		Induction of intracellular calcium accumulation in HEK293 cells at 1 uM by Fluo-4 dye based fluorescence spectrophotometric assay	30419493
1321N1	Function assay		30 mins	Agonist activity at GFP tagged-human P2Y11 receptor expressed in human 1321N1 cells assessed as elevation in calcium level after 30 mins by fluorescence spectrophotometric analysis, EC50=3.3μM.	21090681
1321N1	Function assay		30 mins	Antagonist activity at human P2X4 receptor expressed in 1321N1 cells assessed as inhibition of ATP-induced cytosolic calcium influx compound preincubated for 30 mins before ATP treatment by Fluo-4 AM fluorescence method, EC50=1μM.	23075067
1321N1	Function assay		30 mins	Agonist activity at human P2Y1 receptor expressed in human 1321N1 cells assessed as increase of intracellular calcium level after 30 mins using fura-2 AM by fluorescence assay, EC50=0.85μM.	24846781
1321N1	Function assay		30 mins	Agonist activity at GFP tagged-human P2Y1 receptor expressed in human 1321N1 cells assessed as elevation in calcium level after 30 mins by fluorescence spectrophotometric analysis, EC50=0.15μM.	21090681
THLP1	Function assay			Agonist activity at NLRP3 in human THLP1-null cells assessed as induction of LPS-induced IL-1beta secretion by HEK-Blue reporter cell based assay	28829599
1321N1	Function assay			Antagonistic activity at human recombinant P2Y4 receptor expressed in 1321N1 cells	17302398
1321N1	Function assay			Agonist activity by measuring inositol phosphate accumulation in 1321N1 human astrocytoma cells stably expressing human P2Y purinoceptor 11, EC50=17.3μM.	11985476
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor R272A mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=11.7μM.	19419204
1321N1	Function assay			Agonist activity at GFP-tagged human P2Y11R transfected in human 1321N1 cells assessed as increase in intracellular Ca2+ level by fura 2/AM probe-based fluorescence assay, EC50=6.7μM.	26447940
1321N1	Function assay			Agonist activity at human GFP-tagged P2Y11R transfected in human 1321N1 cells assessed as induction of intracellular calcium mobilization by fluorescence assay, EC50=6.7μM.	23751098
Sf9	Function assay			Binding affinity to recombinant human biotinylated N-terminal GST-tagged autophosphorylated TAK1 (1 to 303 residues) fused with TAB1 (437 to 504 residues) expressed in baculovirus infected sf9 cells by SPR assay, Kd=2.8μM.	28109791
1321N1	Function assay			Accumulation of inositol phosphate in 1321N1 astrocytoma cells expressing human P2Y1 purinoceptor, EC50=1.5μM.	11985476
Sf9	Function assay			Binding affinity to 6XHis-tagged human JAK2 JH2 domain V617F mutant (513 to 827 residues) expressed in baculovirus infected Sf9 cells by MANT-ATP binding assay, Kd=1.3μM.	32329617
Sf9	Function assay			Binding affinity to wild type 6XHis-tagged human JAK2 JH2 domain (513 to 827 residues) expressed in baculovirus infected Sf9 cells by MANT-ATP binding assay, Kd=1.3μM.	32329617
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor C278S mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=1μM.	19419204
1321N1	Function assay			Agonist activity at GFP-tagged human P2Y1R transfected in human 1321N1 cells assessed as increase in intracellular Ca2+ level by fura 2/AM probe-based fluorescence assay, EC50=0.85μM.	26447940
1321N1	Function assay			Agonist activity at human GFP-tagged P2Y1R transfected in human 1321N1 cells assessed as induction of intracellular calcium mobilization by fluorescence assay, EC50=0.85μM.	23751098
1321N1	Function assay			Antagonistic activity at human recombinant P2Y4 receptor expressed in 1321N1 cells, Kb=0.708μM.	17302398
HEK 293	Function assay			Concentration required for calcium mobilization at rat purinergic 2Y1 receptor expressed in HEK 293 cells, EC50=0.59μM.	15317453
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor Y118A mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.299μM.	19419204
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor R177A_R180A double mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.239μM.	19419204
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor R180A mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.237μM.	19419204
BEA	Function assay			Agonist activity at P2Y2 receptor in BEA cells (unknown origin), EC50=0.2μM.	29254895
CF/T43	Function assay			Agonist activity at P2Y2 receptor in human CF/T43 cells, EC50=0.2μM.	29254895
HEK293	Function assay			Agonist activity at P2Y1 receptor expressed in human HEK293 cells, EC50=0.2μM.	20175517
HEK293	Function assay			Agonist activity at rat P2Y1R expressed in HEK293 cells assessed as release of intracellular calcium by fluorescence based assay, EC50=0.2μM.	22873688
NG108-15	Function assay			Agonist activity at P2Y2 receptor in NG108-15 cells (unknown origin), EC50=0.18μM.	29254895
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor R177A mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.183μM.	19419204
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor Y198A mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.12μM.	19419204
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor R194H mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.111μM.	19419204
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged wild type 3 human P2Y2 receptor expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.0958μM.	19419204
1321N1	Function assay			Agonist activity at human recombinant P2Y2 receptor expressed in 1321N1 cells assessed as stimulation of phospholipase C, EC50=0.085μM.	17302398
1321N1	Function assay			Agonist activity at human P2Y2 receptor expressed in human 1321N1 cells coexpressing phospholipase C-activating G protein assessed as inositol phosphate production by scintillation proximity assay, EC50=0.085μM.	19419868
1321N1	Function assay			Agonist activity by measuring inositol phosphate accumulation in 1321N1 human astrocytoma cells stably expressing human P2Y purinoceptor 2, EC50=0.085μM.	11985476
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged wild type 4 human P2Y2 receptor expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.0631μM.	19419204
1321N1	Function assay			Agonist activity at N-terminal HA epitope-tagged human P2Y2 receptor Y114A mutant expressed in human 1321N1 cells assessed as increase in intracellular calcium level, EC50=0.0301μM.	19419204
BL21(DE3)	Function assay			Activity of Bacillus subtilis N-terminal His-tagged ANT(6) expressed in Escherichia coli BL21(DE3) cells assessed as adenylated product by Michaelis-Menten kinetics	ChEMBL
点击查看更多细胞系数据

生物活性

产品描述	ATP disodium是三磷酸腺苷的钠盐形式，是一种多功能的三磷酸核苷。

体外研究(In Vitro)
体外研究活性	ATP释放和自分泌反馈通过P2Y2和A3受体提供信号放大，控制梯度感测和中性粒细胞的迁移。^[1] ATP导致活性氧（ROS）的产生，刺激磷脂酰肌醇3-激酶（PI3K）途径和随后的Akt和ERK1/2的活化。ATP依赖性ROS生成和PI3K激活还刺激氧化性应激反应所需的基因的转录。ATP介导的ROS依赖性PI3K为活化胱天蛋白酶-1以及IL-1β和IL-18的分泌所需要。^[2] 在大鼠培养脑胶质细胞中，ATP有力地刺激了TNF-alpha的释放，从TNF-αmRNA表达产生。在小胶质细胞中，ATP诱导的TNF-α释放是Ca（2+）-依赖性，并且持续的Ca（2+）内流和ATP刺激的TNF-α的释放相关。ATP诱导的TNF-α释放为PD 098059所抑制，PD 098059是细胞外信号调控的蛋白激酶（ERK）激酶1（MEK1）的抑制剂，MEK1活化ERK，ATP诱导的TNF-α释放也为SB 203580所抑制，SB 203580是p38促分裂原活化蛋白激酶抑制剂。ATP在细胞外钙离子不存在的情况下迅速激活ERK和p38。^[3] ATP诱导的细胞毒性是通过经典的细胞凋亡改变调控的，包括膜起泡，核缩合，DNA片段化。在小胶质细胞中，ATP但不是其它核苷酸通过P2Z受体介导的途径导致NF-κB的有效的和选择性的激活。^[4]

体内研究(In Vivo)
体内研究活性	在完整小鼠大脑中，ATP调节胶质细胞分支动力学，并且从受损组织和周围的星形胶质细胞释放的ATP调控胶质细胞对损伤的快速反应。^[5]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
临床试验信息 (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT04760860	Not yet recruiting	Dementia With Lewy Bodies	Qiang Zhang\|University of Iowa	October 2024	Phase 1\|Phase 2
NCT05590195	Not yet recruiting	Bacterial Vaginosis\|Bacterial Infections\|Bacterial Vaginosis \| Vaginal \| Microbiology\|Vaginal Infection	Jeremy Burton\|Deerland Enzymes\|Lawson Health Research Institute\|St. Joseph''s Health Care London	May 1 2024	Phase 3
NCT06167512	Not yet recruiting	Multi-organ Failure After Severe Trauma	Assistance Publique - Hôpitaux de Paris	April 1 2024	--

参考文献
[1] Chen Y, et al. Science,?006, 314(5806), 1792-1795. [2] Cruz CM, et al. J Biol Chem,?007, 282(5), 2871-2879. [3] Hide I, et al. J Neurochem,?000, 75(3), 965-972. [4] Ferrari D, et al. J Cell Biol,?997, 139(7), 1635-1643. [5] Davalos D, et al. Nat Neurosci,?005, 8(6), 752-758. + 展开

参考文献

+ 展开

化学信息&溶解度

分子量	551.14	分子式	C₁₀H₁₄N₅O₁₃P₃.2Na
CAS号	987-65-5	SDF	Download ATP disodium SDF
Smiles	C1=NC(=C2C(=N1)N(C=N2)C3C(C(C(O3)COP(=O)(O)OP(=O)([O-])OP(=O)(O)[O-])O)O)N.[Na+].[Na+]
储存条件(自收到货起)	3年-20°C粉状	储备液保存和期限建议分装储备液，避免反复冻融！	1年-80°C溶于溶剂
储存条件(自收到货起)	3年-20°C粉状	储备液保存和期限建议分装储备液，避免反复冻融！	1个月-20°C溶于溶剂

体外溶解度
批次：

Water : 100 mg/mL (181.44 mM)

DMSO : Insoluble ( ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Ethanol : Insoluble

Water : 100 mg/mL (181.44 mM)

DMSO : Insoluble ( ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Ethanol : Insoluble

摩尔浓度计算器

体内溶解度
批次：

现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂

动物体内配方计算器

实验计算

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×

稀释计算器分子量计算器

动物体内配方计算器（澄清溶液）

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 μL 动物数量只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

计算结果：

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH₂O，混匀澄清。

体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。

注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

技术支持

在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题，请给我们留言。

* 必填项

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):