Tubercidin

别名: 7-deaza-adenosine 中文名称：杀结核菌素

目录号：S8095 Purity: 99.52%

Tubercidin (7-deaza-adenosine)，腺苷类似物，是一种核苷抗生素。它可掺入到DNA中，抑制聚合酶，因此抑制DNA复制和RNA、蛋白合成。Tubercidin还具有抗真菌和抗病毒活性。

Tubercidin Chemical Structure

CAS: 69-33-0

规格	价格	库存
5mg	795.26	现货
25mg	2432.67	现货
100mg	5709.09	现货
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产品质控

批次： S809501 DMSO]53 mg/mL]false]Water]Insoluble]false]Ethanol]Insoluble]false 纯度： 99.52%

99.52

Tubercidin相关产品

相关靶点	tRNA synthetase RdRp DNA synthesis helicase ribonucleotide reductase	点击展开
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相关化合物库	FDA药物库天然产物库凋亡分子化合物库 DNA损伤/ DNA修复化合物库细胞周期化合物库	点击展开

细胞实验数据示例

细胞系	实验类型	给药浓度	孵育时间	活性描述	文献信息
L2	Growth inhibition assay	37 uM		Growth inhibition of mouse L2 cells at 37 uM	17977728
HeLaS3	Antiviral assay		2 days	Antiviral activity against Poliovirus infected in human HeLaS3 cells assessed as inhibition of viral replication treated 1 hr before infection measured after 2 days, IC50=0.03μM	20964406
HeLaS3	Cytotoxicity assay		48 hrs	Cytotoxicity against human HeLaS3 cells after 48 hrs by celltiter-glo assay, IC50=0.4μM	20964406
HeLaS3	Cytotoxicity assay		24 hrs	Cytotoxicity against human HeLaS3 cells after 24 hrs by celltiter-glo assay, IC50=0.819μM	20964406
HeLaS3	Cytotoxicity assay		7 hrs	Cytotoxicity against human HeLaS3 cells after 7 hrs by celltiter-glo assay, IC50=1.44μM	20964406
A549	Cytostatic assay		5 days	Cytostatic activity against human A549 cells after 5 days by SRB assay, GI50=0.001μM	21711054
HCT116	Cytostatic assay		5 days	Cytostatic activity against human HCT116 cells after 5 days by SRB assay, GI50=0.001μM	21711054
NCI-H23	Cytostatic assay		5 days	Cytostatic activity against human NCI-H23 cells after 5 days by SRB assay, GI50=0.011μM	21711054
HCT15	Cytostatic assay		5 days	Cytostatic activity against human HCT15 cells after 5 days by SRB assay, GI50=0.011μM	21711054
DU145	Cytostatic assay		5 days	Cytostatic activity against human DU145 cells after 5 days by SRB assay, GI50=0.018μM	21711054
MT4	Cytostatic assay		5 days	Cytostatic activity against human MT4 cells after 5 days by SRB assay, GI50=0.021μM	21711054
PC3	Cytostatic assay		5 days	Cytostatic activity against human PC3 cells after 5 days by SRB assay, GI50=0.048μM	21711054
Hs578	Cytostatic assay		5 days	Cytostatic activity against human Hs578 cells after 5 days by SRB assay, GI50=0.098μM	21711054
MRC5	Antitrypanosomal assay		7 days	Antitrypanosomal activity against nifurtimox-sensitive Trypanosoma cruzi Tulahuen CL2 harboring beta-galactosidase infected in human MRC5 cells after 7 days by resazurin dye-based assay, EC50=0.34μM	30677668
MRC5	Cytotoxicity assay		3 days	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 3 days by resazurin dye-based assay, EC50=2.23μM	30677668
Vero	Function assay		5 days	Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by measuring reduction in virus-induced cytopathic effect after 5 days by MTS assay, EC50=1.3μM	31549836
L1210	Function assay			Concentration required to decrease the growth rate to 50% of control was evaluated by determining their ability to inhibit growth of L1210 cells in vitro, IC50=0.04μM	1310744
H.Ep.2	Function assay			Concentration required to decrease the growth rate to 50% of control was evaluated by determining their ability to inhibit growth of H.Ep.2 cells in vitro, IC50=0.06μM	1310744
HFF cells	Cytotoxicity assay			Cytotoxicity against uninfected human foreskin fibroblast(HFF cells), IC50=0.4μM	2846837
KB cells	Cytotoxicity assay			Cytotoxicity against human neoplastic cell line(KB cells), IC50=0.6μM	2846837
L1210	Cytotoxicity assay			In vitro cytotoxicity was evaluated against the L1210 Murine leukemic cells, IC50=0.04μM	2913300
HFF cells	Cytotoxicity assay			Cytotoxicity was evaluated against the Human diploid cells (HFF), IC50=0.3μM	2913300
H.Ep.2	Function assay			Compound was evaluated for cytotoxicity against H.Ep.-2 cells, and concentration required to inhibit the growth of treated cells to 50% of untreated control. , IC50=0.002μM	6708054
L1210	Function assay			Tested for in vitro cell growth inhibition of L1210 cells, IC50=0.043μM	8254613
H.Ep.2	Function assay			Tested for in vitro cell growth inhibition of H. Ep. 2 cells, IC50=0.06μM	8254613
HEK293	Function assay			Stabilization of Pdcd4 expressed in human HEK293 cells assessed as inhibition of TPA-induced degradation by luciferase reporter assay, IC50=0.88μM	21870828
Vero	Function assay			Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by methylene blue staining based by Plaque reduction assay, EC50=1.3μM	31549836
Vero	Function assay			Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by Alexa Fluor 488/DAPI staining based assay, EC50=1.3μM	31549836
Vero	Function assay			Inhibition of RNA-dependent RNA polymerase in Zika virus MR766 infected in African green monkey Vero cells assessed as antiviral activity by measuring reduction in virus-yield, EC50=1.3μM	31549836
点击查看更多细胞系数据

生物活性

产品描述	Tubercidin (7-deaza-adenosine)，腺苷类似物，是一种核苷抗生素。它可掺入到DNA中，抑制聚合酶，因此抑制DNA复制和RNA、蛋白合成。Tubercidin还具有抗真菌和抗病毒活性。

体外研究(In Vitro)
体外研究活性	连续14天的tubercidin处理对CFU-GM和BFU-E都具有高度毒性作用。在CFU-GM和BFU-E中，tubercidin的IC50分别为3.4±1.7和3.7±0.2 nM。在体外实验中，Tubercidin对髓系和红系人源骨髓组细胞具有浓度依赖式的抑制作用^[1]。
细胞实验	细胞系	人骨髓细胞
	浓度	10 nM
	孵育时间	14天
	方法	从健康人体中获得并分离出人源骨髓细胞。用10 nM tubercidin处理这些细胞，加入（或不加入）不同浓度的NBMPR-P。将细胞这样连续处理14天（37℃，5% C02-95% air）。在倒置显微镜下对CFU-GM和BFU-E进行计数。

体内研究(In Vivo)
体内研究活性	连续4天，每天向小鼠注射5mg/kg的tubercidin将使小鼠死亡。一些研究表明，tubercidin所导致的死亡可能是由于其对肝脏和肾脏的损害，或者极小可能是对胰脏的损伤。将tubercidin和NBMPR-P共注射可保护小鼠免于死亡^[1]。
动物实验	Animal Models	CD1小鼠
	Dosages	5 mg/kg
	Administration	腹腔注射

参考文献
[1] el Kouni MH, et al. Antimicrob Agents Chemother. 1989, 33(6):824-827.

参考文献

[1] el Kouni MH, et al. Antimicrob Agents Chemother. 1989, 33(6):824-827.

化学信息&溶解度

分子量	266.25	分子式	C₁₁H₁₄N₄O₄
CAS号	69-33-0	SDF	Download Tubercidin SDF
Smiles	C1=CN(C2=NC=NC(=C21)N)C3C(C(C(O3)CO)O)O
储存条件(自收到货起)	3年-20°C粉状	储备液保存和期限建议分装储备液，避免反复冻融！	1年-80°C溶于溶剂
储存条件(自收到货起)	3年-20°C粉状	储备液保存和期限建议分装储备液，避免反复冻融！	1个月-20°C溶于溶剂

体外溶解度
批次：

DMSO : 53 mg/mL ( (199.06 mM) ；DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Water : Insoluble

Ethanol : Insoluble

摩尔浓度计算器

体内溶解度
批次：

现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂

动物体内配方计算器

实验计算

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×

稀释计算器分子量计算器

动物体内配方计算器（澄清溶液）

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 μL 动物数量只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

计算结果：

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH₂O，混匀澄清。

体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。

注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

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